GARS Antibody - #DF7340

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Product: GARS Antibody
Catalog: DF7340
Description: Rabbit polyclonal antibody to GARS
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 75kDa; 83kD(Calculated).
Uniprot: P41250
RRID: AB_2839278

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
GARS Antibody detects endogenous levels of total GARS.
RRID:
AB_2839278
Cite Format: Affinity Biosciences Cat# DF7340, RRID:AB_2839278.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AP 4 A synthetase; Charcot Marie Tooth neuropathy 2D; Charcot Marie Tooth neuropathy neuronal type D; CMT2D; Diadenosine tetraphosphate synthetase; DSMAV; EC 6.1.1.14; Glycine tRNA ligase; Glycyl tRNA synthetase; GlyRS; HMN5; SMAD1;

Immunogens

Immunogen:
Uniprot:

P41250(GARS_HUMAN) >>Visit HPA database.

Gene(ID):
GARS(2617)
Expression:
P41250 GARS_HUMAN:

Widely expressed, including in brain and spinal cord.

Description:
This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.
Sequence:
MPSPRPVLLRGARAALLLLLPPRLLARPSLLLRRSLSAASCPPISLPAAASRSSMDGAGAEEVLAPLRLAVRQQGDLVRKLKEDKAPQVDVDKAVAELKARKRVLEAKELALQPKDDIVDRAKMEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDKKCSVEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPITGNDLSPPVSFNLMFKTFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPSEKDHPKFQNVADLHLYLYSAKAQVSGQSARKMRLGDAVEQGVINNTVLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPSKGAIGKAYKKDAKLVMEYLAICDECYITEMEMLLNEKGEFTIETEGKTFQLTKDMINVKRFQKTLYVEEVVPNVIEPSFGLGRIMYTVFEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEALTRHGVSHKVDDSSGSIGRRYARTDEIGVAFGVTIDFDTVNKTPHTATLRDRDSMRQIRAEISELPSIVQDLANGNITWADVEARYPLFEGQETGKKETIEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P41250 As Substrate

Site PTM Type Enzyme
S29 Phosphorylation
S35 Phosphorylation
S37 Phosphorylation
S53 Phosphorylation
S54 Phosphorylation
K80 Ubiquitination
K82 Sumoylation
K82 Ubiquitination
K85 Ubiquitination
K93 Acetylation
K93 Ubiquitination
K99 Acetylation
K99 Ubiquitination
K108 Acetylation
K108 Ubiquitination
K115 Acetylation
K115 Ubiquitination
K123 Ubiquitination
K129 Ubiquitination
Y141 Phosphorylation
S145 Phosphorylation
Y148 Phosphorylation
T191 Phosphorylation
S192 Phosphorylation
K197 Ubiquitination
K204 Acetylation
K204 Ubiquitination
K207 Ubiquitination
K219 Acetylation
K219 Ubiquitination
K224 Ubiquitination
K309 Acetylation
K309 Ubiquitination
K318 Ubiquitination
S335 Phosphorylation
S377 Phosphorylation
K379 Ubiquitination
Y414 Phosphorylation
K419 Ubiquitination
S423 Phosphorylation
K426 Ubiquitination
Y453 Phosphorylation
C466 S-Nitrosylation
Y467 Phosphorylation
C471 S-Nitrosylation
K484 Acetylation
K484 Ubiquitination
K487 Ubiquitination
S500 Phosphorylation
K501 Acetylation
K501 Ubiquitination
K506 Ubiquitination
Y518 Phosphorylation
K547 Ubiquitination
T548 Phosphorylation
T552 Phosphorylation
K553 Ubiquitination
K559 Acetylation
K559 Ubiquitination
K563 Ubiquitination
R596 Methylation
S606 Phosphorylation
K646 Ubiquitination
S650 Phosphorylation
S653 Phosphorylation
R687 Methylation
K733 Ubiquitination
K734 Ubiquitination
T736 Phosphorylation

Research Backgrounds

Function:

Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.

Subcellular Location:

Cytoplasm. Mitochondrion. Cell projection>Axon. Secreted. Secreted>Extracellular exosome.
Note: In transfected COS7 cells, not detected in mitochondria, nor in Golgi apparatus (PubMed:17035524). Secreted by motor neuron, possibly through the exosome pathway (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, including in brain and spinal cord.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the class-II aminoacyl-tRNA synthetase family.

Research Fields

· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.

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