NQO2 Antibody - #DF7342

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Product: NQO2 Antibody
Catalog: DF7342
Description: Rabbit polyclonal antibody to NQO2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 26kDa; 26kD(Calculated).
Uniprot: P16083
RRID: AB_2839280

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Horse(91%), Rabbit(100%), Dog(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
NQO2 Antibody detects endogenous levels of total NQO2.
RRID:
AB_2839280
Cite Format: Affinity Biosciences Cat# DF7342, RRID:AB_2839280.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DHQV; DIA6; EC 1.10.99.2; MGC94180; NAD(P)H dehydrogenase quinone 2; NAD(P)H menadione oxidoreductase 1 dioxin inducible 2; NAD(P)H menadione oxidoreductase 2 dioxin inducible; NMOR2; NQO 2; NQO2; NQO2_HUMAN; NRH dehydrogenase [quinone] 2; NRH dehydrogenase; NRH:quinone oxidoreductase 2; OTTHUMP00000015948; OTTHUMP00000015949; OTTHUMP00000015953; Ox 2; Ox2; QR2; Quinone; Quinone reductase 2; Ribosyldihydronicotinamide dehydrogenase [quinone]; Ribosyldihydronicotinamide dehydrogenase;

Immunogens

Immunogen:
Uniprot:

P16083(NQO2_HUMAN) >>Visit HPA database.

Gene(ID):
NQO2(4835)
Description:
NQO2 (EC 1.10.99.2) is a flavoprotein that catalyzes the 2-electron reduction of various quinones, redox dyes, and the vitamin K menadione. NQO2 predominantly uses dihydronicotinamide riboside (NRH) as the electron donor (summary by Wu et al., 1997 [PubMed 9367528]).
Sequence:
MAGKKVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNLEPRATDKDITGTLSNPEVFNYGVETHEAYKQRSLASDITDEQKKVREADLVIFQFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQGKLALLSVTTGGTAEMYTKTGVNGDSRYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEERKGMVAAWSQRLQTIWKEEPIPCTAHWHFGQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Horse
91
Chicken
91
Pig
83
Bovine
75
Sheep
75
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16083 As Substrate

Site PTM Type Enzyme
K16 Ubiquitination
S21 Phosphorylation
K23 Ubiquitination
S31 Phosphorylation
K54 Ubiquitination
K77 Ubiquitination
S80 Phosphorylation
K90 Ubiquitination
Y133 Phosphorylation
S135 Phosphorylation
T148 Phosphorylation
Y156 Phosphorylation
K158 Ubiquitination
S197 Phosphorylation
K217 Ubiquitination

Research Backgrounds

Function:

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the NAD(P)H dehydrogenase (quinone) family.

References

1). Idebenone attenuates cerebral inflammatory injury in ischemia and reperfusion via dampening NLRP3 inflammasome activity. MOLECULAR IMMUNOLOGY, 2020 (PubMed: 32438202) [IF=3.2]

Application: WB    Species: mouse    Sample: BV3 cells

Fig. 3. |Idebenone inhibited NLRP3 signaling in BV2 cells under OGD/R conditions. (a) Immunoblot analysis of NQO1, NQO2, NOX2 and NLRP3 signaling in BV2 cells with or without treatment of idebenone after OGD/R.

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