GDI1 Antibody - #DF7352

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Product: GDI1 Antibody
Catalog: DF7352
Description: Rabbit polyclonal antibody to GDI1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 55kDa; 51kD(Calculated).
Uniprot: P31150
RRID: AB_2839290

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
GDI1 Antibody detects endogenous levels of total GDI1.
RRID:
AB_2839290
Cite Format: Affinity Biosciences Cat# DF7352, RRID:AB_2839290.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1A; FLJ41411; GDI-1; gdi1; GDIA_HUMAN; GDIL; GDP dissociation inhibitor 1; Guanosine diphosphate dissociation inhibitor 1; Mental retardation X-linked 41; Mental retardation X-linked 48; MRX41; MRX48; Oligophrenin 2; Oligophrenin-2; OPHN2; Protein XAP 4; Protein XAP-4; Protein XAP4; Rab GDI alpha; Rab GDP dissociation inhibitor alpha; RABGD1A; RABGDIA; XAP 4;

Immunogens

Immunogen:
Uniprot:

P31150(GDIA_HUMAN) >>Visit HPA database.

Gene(ID):
GDI1(2664)
Expression:
P31150 GDIA_HUMAN:

Brain; predominant in neural and sensory tissues.

Description:
GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI1 is expressed primarily in neural and sensory tissues. Mutations in GDI1 have been linked to X-linked nonspecific mental retardation.
Sequence:
MDEEYDVIVLGTGLTECILSGIMSVNGKKVLHMDRNPYYGGESSSITPLEELYKRFQLLEGPPESMGRGRDWNVDLIPKFLMANGQLVKMLLYTEVTRYLDFKVVEGSFVYKGGKIYKVPSTETEALASNLMGMFEKRRFRKFLVFVANFDENDPKTFEGVDPQTTSMRDVYRKFDLGQDVIDFTGHALALYRTDDYLDQPCLETVNRIKLYSESLARYGKSPYLYPLYGLGELPQGFARLSAIYGGTYMLNKPVDDIIMENGKVVGVKSEGEVARCKQLICDPSYIPDRVRKAGQVIRIICILSHPIKNTNDANSCQIIIPQNQVNRKSDIYVCMISYAHNVAAQGKYIAIASTTVETTDPEKEVEPALELLEPIDQKFVAISDLYEPIDDGCESQVFCSCSYDATTHFETTCNDIKDIYKRMAGTAFDFENMKRKQNDVFGEAEQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31150 As Substrate

Site PTM Type Enzyme
K29 Ubiquitination
R35 Methylation
Y38 Phosphorylation
S43 Phosphorylation
S45 Phosphorylation
K54 Ubiquitination
S65 Phosphorylation
R68 Methylation
R70 Methylation
K79 Ubiquitination
Y93 Phosphorylation
T94 Phosphorylation
Y99 Phosphorylation
Y111 Phosphorylation
K112 Ubiquitination
Y117 Phosphorylation
K137 Ubiquitination
R169 Methylation
K174 Ubiquitination
K210 Ubiquitination
S215 Phosphorylation
K221 Acetylation
K221 Methylation
K221 Ubiquitination
S222 Phosphorylation
Y224 Phosphorylation
Y226 Phosphorylation
Y229 Phosphorylation
K253 Ubiquitination
K264 Methylation
K269 Acetylation
K269 Ubiquitination
K278 Ubiquitination
R290 Methylation
K309 Ubiquitination
S316 Phosphorylation
C317 S-Nitrosylation
Y333 Phosphorylation
S338 Phosphorylation
Y339 Phosphorylation
Y349 Phosphorylation
T413 Phosphorylation
K435 Ubiquitination
K437 Ubiquitination

Research Backgrounds

Function:

Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Promotes the dissociation of GDP-bound Rab proteins from the membrane and inhibits their activation. Promotes the dissociation of RAB1A, RAB3A, RAB5A and RAB10 from membranes.

Subcellular Location:

Cytoplasm. Golgi apparatus>trans-Golgi network.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain; predominant in neural and sensory tissues.

Subunit Structure:

Interacts with RHOH. Interacts with the non-phosphorylated forms of RAB1A, RAB3A, RAB5A, RAB5B, RAB5C, RAB8A, RAB8B, RAB10, RAB12, RAB35, and RAB43.

Family&Domains:

Belongs to the Rab GDI family.

Restrictive clause

 

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