Myoglobin Antibody - #DF7358
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| Product: | Myoglobin Antibody |
| Catalog: | DF7358 |
| Description: | Rabbit polyclonal antibody to Myoglobin |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit |
| Mol.Wt.: | 17kDa; 17kD(Calculated). |
| Uniprot: | P02144 |
| RRID: | AB_2839296 |
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
Myoglobin Antibody detects endogenous levels of total Myoglobin.
RRID:
AB_2839296
Cite Format: Affinity Biosciences Cat# DF7358, RRID:AB_2839296.
Cite Format: Affinity Biosciences Cat# DF7358, RRID:AB_2839296.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
MB; MGC13548; MYG_HUMAN; Myoglobin; PVALB;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Description:
This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported.
Sequence:
- P02144 MYG_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Rabbit
100
Pig
100
Horse
88
Bovine
88
Sheep
88
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P02144 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| K17 | Acetylation | Uniprot | |
| K46 | Acetylation | Uniprot | |
| K48 | Acetylation | Uniprot | |
| K51 | Acetylation | Uniprot | |
| K57 | Acetylation | Uniprot | |
| S59 | Phosphorylation | Uniprot | |
| K63 | Acetylation | Uniprot | |
| K64 | Acetylation | Uniprot | |
| T68 | Phosphorylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| K78 | Acetylation | Uniprot | |
| Y104 | Phosphorylation | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| Y147 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Family&Domains:
Belongs to the globin family.
References
1). A Bama miniature pig model of monoallelic TSC1 mutation for human tuberous sclerosis complex. Journal of Genetics and Genomics, 2020
(PubMed: 33612456)
[IF=6.6]
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