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Product Info

ELISA 1:10000, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Monoclonal [AFB1850]
CTCF antibody detects endogenous levels of total CTCF.
Cite Format: Affinity Biosciences Cat# BF0501, RRID:AB_2833874.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


11 zinc finger protein; 11 zinc finger transcriptional repressor; 11-zinc finger protein; CCCTC binding factor (zinc finger protein); CCCTC binding factor; CCCTC-binding factor; Ctcf; CTCF_HUMAN; CTCFL paralog; MRD21; Transcriptional repressor CTCF;



Purified recombinant fragment of human CTCF expressed in E. Coli.


Ubiquitous. Absent in primary spermatocytes.

This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.Tissue specificity: Ubiquitous. Absent in primary spermatocytes.

PTMs - P49711 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K20 Acetylation
K74 Sumoylation
Y197 Phosphorylation
S224 Phosphorylation
Y226 Phosphorylation
S287 Phosphorylation
T289 Phosphorylation
T305 Phosphorylation
T307 Phosphorylation
T317 Phosphorylation
T319 Phosphorylation
T346 Phosphorylation
K352 Methylation
T374 Phosphorylation
S382 Phosphorylation
T400 Phosphorylation
S402 Phosphorylation
Y407 Phosphorylation
Y410 Phosphorylation
K423 Ubiquitination
K429 Ubiquitination
T431 Phosphorylation
K436 Ubiquitination
K449 Ubiquitination
S450 Phosphorylation
K458 Ubiquitination
S461 Phosphorylation
Y462 Phosphorylation
K487 Ubiquitination
K496 Ubiquitination
T516 Phosphorylation
T518 Phosphorylation
S526 Phosphorylation
K543 Ubiquitination
Y545 Phosphorylation
S558 Phosphorylation
C577 S-Nitrosylation
S604 Phosphorylation
S609 Phosphorylation
S610 Phosphorylation
S612 Phosphorylation
T642 Phosphorylation
K689 Sumoylation
T709 Phosphorylation
T717 Phosphorylation
S723 Phosphorylation

Research Backgrounds


Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays an important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. Plays a role in the recruitment of CENPE to the pericentromeric/centromeric regions of the chromosome during mitosis.


Sumoylated on Lys-74 and Lys-689; sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter.

Subcellular Location:

Nucleus>Nucleoplasm. Chromosome. Chromosome>Centromere.
Note: May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Absent in primary spermatocytes.

Subunit Structure:

Interacts with CHD8. Interacts with LLPH (By similarity). Interacts with CENPE.


The 11 zinc fingers are highly conserved among vertebrates, exhibiting almost identical amino acid sequences. Different subsets or combination of individual zinc fingers gives the ability to CTCF to recognize multiple DNA target sites.

Belongs to the CTCF zinc-finger protein family.

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