PSRC1 Antibody - #DF7366

(3)  
Product: PSRC1 Antibody
Catalog: DF7366
Description: Rabbit polyclonal antibody to PSRC1
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Rabbit, Dog
Mol.Wt.: 38kDa; 39kD(Calculated).
Uniprot: Q6PGN9
RRID: AB_2839304

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Rabbit(83%), Dog(82%)
Clonality:
Polyclonal
Specificity:
PSRC1 Antibody detects endogenous levels of total PSRC1.
RRID:
AB_2839304
Cite Format: Affinity Biosciences Cat# DF7366, RRID:AB_2839304.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DDA3; DDA3, mouse, homolog of; Differential display and activated by p53; FP3214; OTTHUMP00000013785; OTTHUMP00000013786; p53-regulated DDA3; Proline/serine-rich coiled-coil 1; Proline/serine-rich coiled-coil protein 1; PSRC1; PSRC1_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q6PGN9(PSRC1_HUMAN) >>Visit HPA database.

Gene(ID):
PSRC1(84722)
Expression:
Q6PGN9 PSRC1_HUMAN:

Widely expressed in adult and fetal tissues, with highest expression in the adult brain and fetal thymus. Not detected in adult skeletal muscle.

Description:
This gene encodes a proline-rich protein. Studies of this gene and the related mouse gene suggest that this gene is regulated by p53 and may participate in p53-mediated growth suppression. The encoded protein may function as a microtubule destabilizing protein that controls spindle dynamics and mitotic progression by recruiting and regulating microtubule depolymerases. At least one genetic variation in this gene has been associated with decreased serum levels of low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding different isoforms have been described.
Sequence:
MEDLEEDVRFIVDETLDFGGLSPSDSREEEDITVLVTPEKPLRRGLSHRSDPNAVAPAPQGVRLSLGPLSPEKLEEILDEANRLAAQLEQCALQDRESAGEGLGPRRVKPSPRRETFVLKDSPVRDLLPTVNSLTRSTPSPSSLTPRLRSNDRKGSVRALRATSGKRPSNMKRESPTCNLFPASKSPASSPLTRSTPPVRGRAGPSGRAAASEETRAAKLRVSGSGEFVGLTLKFLHPSPPGPPTPIRSVLAPQPSTSNSQRLPRPQGAAAKSSSQLPIPSAIPRPASRMPLTSRSVPPGRGALPPDSLSTRKGLPRPSTAGHRVRESGHKVPVSQRLNLPVMGATRSNLQPPRKVAVPGPTR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
83
Dog
82
Horse
75
Pig
67
Bovine
58
Sheep
58
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6PGN9 As Substrate

Site PTM Type Enzyme
S22 Phosphorylation
T37 Phosphorylation
S47 Phosphorylation
S65 Phosphorylation
S70 Phosphorylation
S98 Phosphorylation
S111 Phosphorylation
T116 Phosphorylation
S122 Phosphorylation
S133 Phosphorylation
S137 Phosphorylation
T138 Phosphorylation
S140 Phosphorylation
S142 Phosphorylation
S143 Phosphorylation
T145 Phosphorylation
S175 Phosphorylation
T177 Phosphorylation
S184 Phosphorylation
S186 Phosphorylation
S189 Phosphorylation
S190 Phosphorylation
T193 Phosphorylation
S212 Phosphorylation
S223 Phosphorylation
S225 Phosphorylation
S239 Phosphorylation
T245 Phosphorylation
R301 Methylation
S308 Phosphorylation
S310 Phosphorylation
T311 Phosphorylation
T346 Phosphorylation

Research Backgrounds

Function:

Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate, and for normal rate of chromosomal segregation during anaphase. Plays a role in the regulation of mitotic spindle dynamics. Increases the rate of turnover of microtubules on metaphase spindles, and contributes to the generation of normal tension across sister kinetochores. Recruits KIF2A and ANKRD53 to the mitotic spindle and spindle poles. May participate in p53/TP53-regulated growth suppression.

PTMs:

Phosphorylated during mitosis.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm>Cytoskeleton>Spindle pole.
Note: Detected at the mitotic spindle and spindle poles. Diffusely distributed throughout the cell during interphase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed in adult and fetal tissues, with highest expression in the adult brain and fetal thymus. Not detected in adult skeletal muscle.

Subunit Structure:

Interacts with APC2 (By similarity). Interacts with KIF2A. Interacts with ANKRD53; recruits ANKRD53 to the spindle during mitosis.

Family&Domains:

Belongs to the PSRC1 family.

Restrictive clause

 

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