TLE1 Antibody - #DF7380
| Product: | TLE1 Antibody |
| Catalog: | DF7380 |
| Description: | Rabbit polyclonal antibody to TLE1 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 83kDa; 83kD(Calculated). |
| Uniprot: | Q04724 |
| RRID: | AB_2839318 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF7380, RRID:AB_2839318.
Fold/Unfold
E(Sp1) homolog; Enhancer of split groucho 1; Enhancer of split groucho like protein 1; Enhancer of split groucho-like protein 1; ESG; ESG1; GRG1; TLE1; TLE1_HUMAN; Transducin like enhancer of split 1 (E(sp1) homolog Drosophila); Transducin like enhancer of split 1; Transducin like enhancer of split 1 homolog of Drosophila E(sp1); Transducin like enhancer protein 1; Transducin-like enhancer protein 1;
Immunogens
- Q04724 TLE1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFPQSRHPTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGQQQLQAQHLSHGHGPPVPLTPHPSGLQPPGIPPLGGSAGLLALSSALSGQSHLAIKDDKKHHDAEHHRDREPGTSNSLLVPDSLRGTDKRRNGPEFSNDIKKRKVDDKDSSHYDSDGDKSDDNLVVDVSNEDPSSPRASPAHSPRENGIDKNRLLKKDASSSPASTASSASSTSLKSKEMSLHEKASTPVLKSSTPTPRSDMPTPGTSATPGLRPGLGKPPAIDPLVNQAAAGLRTPLAVPGPYPAPFGMVPHAGMNGELTSPGAAYASLHNMSPQMSAAAAAAAVVAYGRSPMVGFDPPPHMRVPTIPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGDKKATVYEVIY
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q04724 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| R6 | Methylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S244 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S259 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| K281 | Acetylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| S286 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| T290 | Phosphorylation | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| T312 | Phosphorylation | Uniprot | |
| T319 | Phosphorylation | Uniprot | |
| R338 | Methylation | Uniprot | |
| K343 | Methylation | Uniprot | |
| R359 | Methylation | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| R641 | Methylation | Uniprot | |
| Y692 | Phosphorylation | Uniprot | |
| S699 | Phosphorylation | Uniprot | |
| S703 | Phosphorylation | Uniprot | |
| K718 | Acetylation | Uniprot | |
| K718 | Ubiquitination | Uniprot | |
| K761 | Ubiquitination | Uniprot | |
| K762 | Sumoylation | Uniprot |
Research Backgrounds
Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
Phosphorylated, probably by CDK1. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1.
Ubiquitinated by XIAP/BIRC4.
Nucleus.
Note: Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components.
In all tissues examined, mostly in brain, liver and muscle.
Homooligomer and heterooligomer with other family members. Binds LEF1, RUNX1, RUNX3, FOXA2, KDM6A, UTY, histone H3, HESX1, ESRRG and the NF-kappa-B subunit RELA. Interacts with HES1 (via WRPW motif). Interacts with SIX3. Interacts with EFNB1.
WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain.
Belongs to the WD repeat Groucho/TLE family.
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