CALCA Antibody - #DF7386

(2)   (5)  
Product: CALCA Antibody
Catalog: DF7386
Description: Rabbit polyclonal antibody to CALCA
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Sheep, Dog, Chicken
Mol.Wt.: 13kDa; 14kD(Calculated).
Uniprot: P06881
RRID: AB_2839324

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Zebrafish(89%), Sheep(83%), Dog(92%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
CALCA Antibody detects endogenous levels of total CALCA.
RRID:
AB_2839324
Cite Format: Affinity Biosciences Cat# DF7386, RRID:AB_2839324.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Calcitonin gene-related peptide 1;Alpha-type CGRP;Calcitonin gene-related peptide I;CGRP-I;CALCA;CALC1;

Immunogens

Immunogen:

A synthesized peptide derived from human CALCA, corresponding to a region within the internal amino acids.

Uniprot:

P06881(CALCA_HUMAN) >>Visit HPA database.

Gene(ID):
CALCA(796)
Description:
This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.
Sequence:
MGFQKFSPFLALSILVLLQAGSLHAAPFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSRIIAQKRACDTATCVTHRLAGLLSRSGGVVKNNFVPTNVGSKAFGRRRRDLQA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
92
Zebrafish
89
Pig
83
Sheep
83
Chicken
83
Horse
0
Bovine
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P06881 As Substrate

Site PTM Type Enzyme
S99 Phosphorylation
S101 Phosphorylation

Research Backgrounds

Function:

CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the calcitonin family.

References

1). Danggui niantong decoction attenuates synovial fibrosis through regulating PI3k/AKT signaling pathway. Journal of ethnopharmacology, 2025 (PubMed: 39832630) [IF=4.8]
2). Activation of oxytocin receptor in the trigeminal ganglion attenuates orofacial ectopic pain attributed to inferior alveolar nerve injury. Journal of Neurophysiology, 2021 (PubMed: 33326336) [IF=2.1]

Application: IF/ICC    Species: Rat    Sample:

Figure 2.Oxytocin receptor (OXTR) wasexpressed in neurons but not glial cells inthe trigeminal ganglion (TG) and spinal tri-geminal nucleus caudalis (SpVc) of inferioralveolar nerve (IAN) transection (IANX)rats.A: in TG slices, arrowheads showcolocalization of NeuN (neuron marker)and OXTR (red, NeuN; green, OXTR; blue,DAPI).B: in TG slices, activated satelliteglial cells (arrows) surrounded OXTR-posi-tive neurons (arrowheads), and there wasno coexpression [red, glialfibrillary acidicprotein (GFAP); green, OXTR; blue, DAPI].C: in TG slices, coexpression of OXTR andcalcitonin gene-related peptide (CGRP) isindicated with arrowheads (red, CGRP;green, OXTR; blue, DAPI).D:inSpVcsli-ces, double immunohistochemistry indi-cates colocalization of NeuN and OXTR(arrowheads: red, NeuN; green, OXTR;blue, DAPI).E:inSpVcslices,immunofluo-rescence (IF) staining shows no OXTR(arrowheads) colocalized with GFAP(arrows: red, GFAP; green, OXTR; blue,DAPI) (n= 3 rats in each group; scale bars,50μm).INFLUENCE OF OXYTOCIN RECEPTOR ON OROFACIAL ECTOPIC PAINJ Neurophysioldoi:10.1152/jn.00646.2020www.jn.org227

Application: WB    Species: Rat    Sample:

Figure 3.Inferior alveolar nerve (IAN) transection (IANX) resulted in upregulated expression of oxytocin receptor (OXTR), calcitonin gene-related peptide(CGRP), IL-1b, and TNFain the trigeminal ganglion (TG) ondays 1, 4,and7after surgery.AandD: representative Western blot (WB) bands of OXTR,CGRP, IL-1b, and TNFaexpression in the trigeminal ganglion (TG) ondays 1, 4,and7after operation.B, C, E,andF: bar graphs show increased OXTR,CGRP, IL-1band TNFaexpression of IANX rats vs. 2 other groups; sham operation did not result in significant change vs. naive rats (P

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.