PRDM14 Antibody | Affinity Biosciences

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PRDM14 Antibody - Western blot analysis of extracts from HepG2 cells, using PRDM14 Antibody.

Product:	PRDM14 Antibody
Catalog:	DF7387
Description:	Rabbit polyclonal antibody to PRDM14
Application:	WB IHC IF/ICC IP CHIP
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	64kDa; 64kD(Calculated).
Uniprot:	Q9GZV8
RRID:	AB_2839325

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200, IP 1:20-1:100, CHIP 1:20-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

PRDM14 Antibody detects endogenous levels of total PRDM14.

RRID:

AB_2839325
Cite Format: Affinity Biosciences Cat# DF7387, RRID:AB_2839325.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

MGC59730; PFM 11; PFM11; PR domain containing 14; PR domain zinc finger protein 14; PR domain-containing protein 14; PRD14_HUMAN; PRDM 14; PRDM14;

Immunogens

Immunogen:

Uniprot:

Q9GZV8(PRD14_HUMAN) >>Visit HPA database.

Gene(ID):

PRDM14(63978)

Expression:

Q9GZV8 PRD14_HUMAN:

Expressed in embryonic stem cells. Tends to be overexpressed in breast cancer (at protein level).

Description:

This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer.

Sequence:

Q9GZV8 PRD14_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALPRPSEAVPQDKVCYPPESSPQNLAAYYTPFPSYGHYRNSLATVEEDFQPFRQLEAAASAAPAMPPFPFRMAPPLLSPGLGLQREPLYDLPWYSKLPPWYPIPHVPREVPPFLSSSHEYAGASSEDLGHQIIGGDNESGPCCGPDTLIPPPPADASLLPEGLRTSQLLPCSPSKQSEDGPKPSNQEGKSPARFQFTEEDLHFVLYGVTPSLEHPASLHHAISGLLVPPDSSGSDSLPQTLDKDSLQLPEGLCLMQTVFGEVPHFGVFCSSFIAKGVRFGPFQGKVVNASEVKTYGDNSVMWEIFEDGHLSHFIDGKGGTGNWMSYVNCARFPKEQNLVAVQCQGHIFYESCKEIHQNQELLVWYGDCYEKFLDIPVSLQVTEPGKQPSGPSEESAEGYRCERCGKVFTYKYYRDKHLKYTPCVDKGDRKFPCSLCKRSFEKRDRLRIHILHVHEKHRPHKCSTCGKCFSQSSSLNKHMRVHSGDRPYQCVYCTKRFTASSILRTHIRQHSGEKPFKCKYCGKSFASHAAHDSHVRRSHKEDDGCSCSICGKIFSDQETFYSHMKFHEDY

PTMs - Q9GZV8 As Substrate

Q9GZV8

Site	PTM Type	Source
Y29	Phosphorylation	Uniprot
Y30	Phosphorylation	Uniprot
Y39	Phosphorylation	Uniprot
S79	Phosphorylation	Uniprot
T166	Phosphorylation	Uniprot
S173	Phosphorylation	Uniprot
S191	Phosphorylation	Uniprot
K387	Ubiquitination	Uniprot
Y414	Phosphorylation	Uniprot

Research Backgrounds

Q9GZV8_PRD14_HUMAN

Function:

Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of embryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. Its association with CBFA2T2 is required for the functions in pluripotency and germ cell formation (By similarity). Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.

Subcellular Location:

Nucleus.

Tissue Specificity:

Expressed in embryonic stem cells. Tends to be overexpressed in breast cancer (at protein level).

Subunit Structure:

Interacts with CBFA2T2.

Family&Domains:

The first 5 zinc fingers, but not the last one, are required for DNA-binding and transcriptional activity.

Belongs to the class V-like SAM-binding methyltransferase superfamily.

Restrictive clause

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For Research Use Only.
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PRDM14 Antibody - #DF7387