KLC1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

KLC1 Antibody - Western blot analysis of extracts from Rat brain, using KLC1 Antibody at 1/1000 dilution.

KLC1 Antibody - DF7392 staining Hela cells by IF/ICC.

Product:	KLC1 Antibody
Catalog:	DF7392
Description:	Rabbit polyclonal antibody to KLC1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Rabbit, Dog, Xenopus
Mol.Wt.:	65kDa; 65kD(Calculated).
Uniprot:	Q07866
RRID:	AB_2839330

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

KLC1 Antibody detects endogenous levels of total KLC1.

RRID:

AB_2839330
Cite Format: Affinity Biosciences Cat# DF7392, RRID:AB_2839330.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Kinesin 2 60/70kDa; Kinesin light chain 1; Kinesin2; KLC 1; KLC; Klc1; KLC1_HUMAN; KNS 2; KNS 2A; KNS2; KNS2A; Medulloblastoma antigen MU MB 2.50; MGC15245;

Immunogens

Immunogen:

Uniprot:

Q07866(KLC1_HUMAN) >>Visit HPA database.

Gene(ID):

KLC1(3831)

Expression:

Q07866 KLC1_HUMAN:

Found in a variety of tissues. Mostly abundant in brain and spine.

Description:

Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named kinesin 2, this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature and/or biological validity of most of these variants have not been determined.

Sequence:

Q07866 KLC1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMMALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLFPNDEDDPGQGIQQQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVEYYYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAETLYKEILTRAHEREFGSVDDENKPIWMHAEEREECKGKQKDGTSFGEYGGWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAAMRSRKQGLDNVHKQRVAEVLNDPENMEKRRSRESLNVDVVKYESGPDGGEEVSMSVEWNGGVSGRASFCGKRQQQQWPGRRHR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Dog

100

Xenopus

100

Rabbit

100

Pig

Horse

Sheep

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q07866 As Substrate

Q07866

Site	PTM Type	Enzyme	Source
Y2	Phosphorylation		Uniprot
Y10	Phosphorylation		Uniprot
K18	Ubiquitination		Uniprot
K54	Ubiquitination		Uniprot
S75	Phosphorylation		Uniprot
S134	Phosphorylation		Uniprot
K146	Ubiquitination		Uniprot
S162	Phosphorylation		Uniprot
Y207	Phosphorylation		Uniprot
K237	Ubiquitination		Uniprot
Y271	Phosphorylation		Uniprot
K272	Acetylation		Uniprot
Y307	Phosphorylation		Uniprot
K309	Ubiquitination		Uniprot
Y360	Phosphorylation		Uniprot
Y369	Phosphorylation		Uniprot
T371	Phosphorylation		Uniprot
K372	Ubiquitination		Uniprot
K384	Ubiquitination		Uniprot
S389	Phosphorylation		Uniprot
K393	Acetylation		Uniprot
K393	Ubiquitination		Uniprot
S418	Phosphorylation		Uniprot
K441	Ubiquitination		Uniprot
S445	Phosphorylation		Uniprot
Y449	Phosphorylation		Uniprot
K454	Methylation		Uniprot
S460	Phosphorylation	P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
T462	Phosphorylation		Uniprot
T464	Phosphorylation		Uniprot
T465	Phosphorylation		Uniprot
K495	Ubiquitination		Uniprot
K503	Ubiquitination		Uniprot
R505	Methylation		Uniprot
K518	Ubiquitination		Uniprot
S521	Phosphorylation	Q13131 (PRKAA1)	Uniprot
S524	Phosphorylation		Uniprot
Y532	Phosphorylation		Uniprot
S557	Phosphorylation		Uniprot

Research Backgrounds

Q07866_KLC1_HUMAN

Function:

Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.

Subcellular Location:

Cell projection>Growth cone. Cytoplasmic vesicle. Cytoplasm>Cytoskeleton.

Tissue Specificity:

Found in a variety of tissues. Mostly abundant in brain and spine.

Subunit Structure:

Oligomeric complex composed of two heavy chains and two light chains. Interacts with SPAG9. Interacts with ATCAY; may link mitochondria to KLC1 and regulate mitochondria localization into neuron projections (By similarity). Interacts (via TPR repeats) with TOR1A; the interaction associates TOR1A with the kinesin oligomeric complex. Interacts with BORCS5. Interacts with MAPK8IP3/JIP3 and NTRK2/TRKB; interaction with NTRK2/TRKB is mediated by MAPK8IP3/JIP3 (By similarity).

(Microbial infection) Interacts with adenovirus hexon-interlacing protein; this interaction leads to capsid disruption at the nuclear pore complex during virus entry into host cell.

Family&Domains:

Belongs to the kinesin light chain family.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

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KLC1 Antibody - #DF7392