PCM1 Antibody - #DF7464

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Product: PCM1 Antibody
Catalog: DF7464
Description: Rabbit polyclonal antibody to PCM1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 228kDa; 229kD(Calculated).
Uniprot: Q15154
RRID: AB_2839401

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
PCM1 Antibody detects endogenous levels of total PCM1.
RRID:
AB_2839401
Cite Format: Affinity Biosciences Cat# DF7464, RRID:AB_2839401.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hPCM-1; PCM-1; pcm1; PCM1_HUMAN; Pericentriolar material 1; Pericentriolar material 1 protein; PTC4;

Immunogens

Immunogen:
Uniprot:

Q15154(PCM1_HUMAN) >>Visit HPA database.

Gene(ID):
PCM1(5108)
Expression:
Q15154 PCM1_HUMAN:

Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid.

Description:
The protein encoded by this gene is a component of centriolar satellites, which are electron dense granules scattered around centrosomes. Inhibition studies show that this protein is essential for the correct localization of several centrosomal proteins, and for anchoring microtubules to the centrosome. Chromosomal aberrations involving this gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma.
Sequence:
MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTNPPNRETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSASPQRSVDQRSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRSAANIRALNMPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSSNRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASLASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESHEKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQNDADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNSNMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDEDKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKAETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQVLQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAHKESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDTESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEEEQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
83
Zebrafish
69
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15154 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T3 Phosphorylation
K39 Ubiquitination
K51 Ubiquitination
S56 Phosphorylation
K58 Ubiquitination
T61 Phosphorylation
S65 Phosphorylation
S68 Phosphorylation
S69 Phosphorylation
T77 Phosphorylation
K78 Ubiquitination
T79 Phosphorylation
T82 Phosphorylation
Y88 Phosphorylation
S90 Phosphorylation
S93 Phosphorylation
K102 Ubiquitination
K104 Ubiquitination
S110 Phosphorylation
S116 Phosphorylation
S119 Phosphorylation
S121 Phosphorylation
T126 Phosphorylation
S135 Phosphorylation
K139 Ubiquitination
K152 Ubiquitination
K154 Ubiquitination
S157 Phosphorylation
T158 Phosphorylation
N159 Phosphorylation
T165 Phosphorylation
S168 Phosphorylation
K172 Ubiquitination
S177 Phosphorylation
S180 Phosphorylation
S190 Phosphorylation
S203 Phosphorylation
Y215 Phosphorylation
K218 Ubiquitination
K229 Ubiquitination
K247 Ubiquitination
K255 Ubiquitination
K258 Ubiquitination
K259 Ubiquitination
K277 Ubiquitination
K278 Ubiquitination
K284 Ubiquitination
S320 Phosphorylation
S356 Phosphorylation
S370 Phosphorylation
S372 Phosphorylation O00444 (PLK4)
T374 Phosphorylation
S378 Phosphorylation
S380 Phosphorylation
K382 Ubiquitination
S384 Phosphorylation
S386 Phosphorylation
K393 Ubiquitination
K399 Acetylation
K399 Ubiquitination
K407 Acetylation
K409 Acetylation
K409 Ubiquitination
K412 Ubiquitination
T419 Phosphorylation
S428 Phosphorylation
S429 Phosphorylation
S431 Phosphorylation
S435 Phosphorylation
K489 Ubiquitination
K492 Ubiquitination
Y509 Phosphorylation
T530 Phosphorylation
S533 Phosphorylation
Y535 Phosphorylation
S537 Phosphorylation
S542 Phosphorylation
T546 Phosphorylation
T556 Phosphorylation
S580 Phosphorylation
C582 S-Nitrosylation
S588 Phosphorylation
S600 Phosphorylation
S634 Phosphorylation
S639 Phosphorylation
S643 Phosphorylation
S644 Phosphorylation
K658 Ubiquitination
K666 Ubiquitination
K668 Ubiquitination
Y700 Phosphorylation
K707 Ubiquitination
T720 Phosphorylation
K722 Ubiquitination
T724 Phosphorylation
K729 Ubiquitination
K733 Ubiquitination
Y735 Phosphorylation
K738 Ubiquitination
K747 Ubiquitination
K754 Ubiquitination
K755 Ubiquitination
K762 Ubiquitination
S776 Phosphorylation
S779 Phosphorylation
T785 Phosphorylation
K786 Ubiquitination
K787 Ubiquitination
S794 Phosphorylation
T795 Phosphorylation
T797 Phosphorylation
S804 Phosphorylation
K807 Ubiquitination
S808 Phosphorylation
S815 Phosphorylation
S816 Phosphorylation
S824 Phosphorylation
K841 Ubiquitination
T859 Phosphorylation
S861 Phosphorylation
S866 Phosphorylation
S869 Phosphorylation
S872 Phosphorylation
T877 Phosphorylation
S881 Phosphorylation
S909 Phosphorylation
K950 Ubiquitination
K954 Ubiquitination
S960 Phosphorylation
Y965 Phosphorylation
K970 Ubiquitination
S977 Phosphorylation
S988 Phosphorylation
S991 Phosphorylation
Y992 Phosphorylation
K996 Ubiquitination
K1007 Ubiquitination
K1076 Ubiquitination
K1092 Ubiquitination
K1096 Ubiquitination
S1107 Phosphorylation
T1174 Phosphorylation
Y1176 Phosphorylation
K1181 Ubiquitination
S1185 Phosphorylation
S1186 Phosphorylation
S1187 Phosphorylation
S1188 Phosphorylation
K1193 Ubiquitination
K1197 Ubiquitination
K1198 Ubiquitination
S1206 Phosphorylation
T1209 Phosphorylation
Y1213 Phosphorylation
K1221 Ubiquitination
K1225 Ubiquitination
T1226 Phosphorylation
S1229 Phosphorylation
S1231 Phosphorylation
K1234 Ubiquitination
K1241 Ubiquitination
S1257 Phosphorylation
S1260 Phosphorylation
S1262 Phosphorylation
S1263 Phosphorylation
T1271 Phosphorylation
T1272 Phosphorylation
T1274 Phosphorylation
K1275 Ubiquitination
K1281 Ubiquitination
S1283 Phosphorylation
S1287 Phosphorylation
S1290 Phosphorylation
K1291 Ubiquitination
T1294 Phosphorylation
T1305 Phosphorylation
K1308 Ubiquitination
Y1316 Phosphorylation
S1318 Phosphorylation
S1320 Phosphorylation
T1324 Phosphorylation
K1329 Ubiquitination
S1335 Phosphorylation
T1338 Phosphorylation
K1345 Ubiquitination
S1348 Phosphorylation
K1350 Ubiquitination
K1357 Ubiquitination
S1373 Phosphorylation
S1435 Phosphorylation
K1440 Ubiquitination
T1451 Phosphorylation
T1460 Phosphorylation
T1468 Phosphorylation
S1494 Phosphorylation
S1497 Phosphorylation
S1499 Phosphorylation
Y1527 Phosphorylation
K1531 Ubiquitination
T1532 Phosphorylation
T1543 Phosphorylation
T1558 Phosphorylation
T1565 Phosphorylation
S1578 Phosphorylation
C1585 S-Nitrosylation
T1590 Phosphorylation
R1595 Methylation
K1602 Ubiquitination
K1609 Ubiquitination
K1639 Ubiquitination
K1643 Ubiquitination

Research Backgrounds

Function:

Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Involved in the biogenesis of cilia.

PTMs:

Ubiquitinated. Undergoes monoubiquitination catalyzed by the E3 ubiquitin-protein ligase MIB1 in proliferating cells, preventing cilia formation. Monoubiquitination by MIB1 is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, resulting in cilia formation initiation.

Variant Ser-159 is phosphorylated.

Phosphorylated on multiple serine and threonine residues by DYRK3 during the G2-to-M transition, after the nuclear-envelope breakdown. Phosphorylation by DYRK3 promotes disassembly of pericentriolar material.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasmic granule. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Recruitment to the centrosome requires microtubules and dynein. The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase. Displaced from centriolar satellites and centrosome in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, in a process that requires p38 MAP kinase signaling.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid.

Subunit Structure:

Self-associates. Interacts with C2CD3 (By similarity). Interacts with BBS4, BBS8, CETN3, HAP1, NDE1, NDEL1, MAP1LC3B, GABARAPAL2, and GABARAP. Interacts with CEP131; the interaction increases in response to ultraviolet light (UV) radiation. Associates with microtubule; association to microtubule is reduced in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, in a process that requires p38 MAP kinase signaling. Interacts with CCDC113. Interacts with SSX2IP (By similarity). Interacts with CCDC13. Interacts with CEP290 (By similarity). Interacts with PARD6A. Interacts with KIAA0753/OFIP, FOPNL/FOR20 and OFD1; the interaction with FOPNL/FOR20 and OFD1 may be mediated by KIAA0753/OFIP. Interacts with CCDC66.

Family&Domains:

Belongs to the PCM1 family.

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