MYO1F Antibody - #DF2210

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Product: MYO1F Antibody
Catalog: DF2210
Description: Rabbit polyclonal antibody to MYO1F
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 125 kDa.; 125kD(Calculated).
Uniprot: O00160
RRID: AB_2839441

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Zebrafish(80%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(90%), Dog(89%), Chicken(90%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
MYO1F Antibody detects endogenous levels of total MYO1F.
RRID:
AB_2839441
Cite Format: Affinity Biosciences Cat# DF2210, RRID:AB_2839441.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Myo1f; MYO1F_HUMAN; Myosin ID; Myosin IF; Myosin-Ie; Unconventional myosin-If;

Immunogens

Immunogen:
Uniprot:

O00160(MYO1F_HUMAN) >>Visit HPA database.

Gene(ID):
MYO1F(4542)
Description:
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments.
Sequence:
MGSKERFHWQSHNVKQSGVDDMVLLPQITEDAIAANLRKRFMDDYIFTYIGSVLISVNPFKQMPYFTDREIDLYQGAAQYENPPHIYALTDNMYRNMLIDCENQCVIISGESGAGKTVAAKYIMGYISKVSGGGEKVQHVKDIILQSNPLLEAFGNAKTVRNNNSSRFGKYFEIQFSRGGEPDGGKISNFLLEKSRVVMQNENERNFHIYYQLLEGASQEQRQNLGLMTPDYYYYLNQSDTYQVDGTDDRSDFGETLSAMQVIGIPPSIQQLVLQLVAGILHLGNISFCEDGNYARVESVDLLAFPAYLLGIDSGRLQEKLTSRKMDSRWGGRSESINVTLNVEQAAYTRDALAKGLYARLFDFLVEAINRAMQKPQEEYSIGVLDIYGFEIFQKNGFEQFCINFVNEKLQQIFIELTLKAEQEEYVQEGIRWTPIQYFNNKVVCDLIENKLSPPGIMSVLDDVCATMHATGGGADQTLLQKLQAAVGTHEHFNSWSAGFVIHHYAGKVSYDVSGFCERNRDVLFSDLIELMQTSEQAFLRMLFPEKLDGDKKGRPSTAGSKIKKQANDLVATLMRCTPHYIRCIKPNETKRPRDWEENRVKHQVEYLGLKENIRVRRAGFAYRRQFAKFLQRYAILTPETWPRWRGDERQGVQHLLRAVNMEPDQYQMGSTKVFVKNPESLFLLEEVRERKFDGFARTIQKAWRRHVAVRKYEEMREEASNILLNKKERRRNSINRNFVGDYLGLEERPELRQFLGKRERVDFADSVTKYDRRFKPIKRDLILTPKCVYVIGREKVKKGPEKGQVCEVLKKKVDIQALRGVSLSTRQDDFFILQEDAADSFLESVFKTEFVSLLCKRFEEATRRPLPLTFSDTLQFRVKKEGWGGGGTRSVTFSRGFGDLAVLKVGGRTLTVSVGDGLPKSSKPTRKGMAKGKPRRSSQAPTRAAPAPPRGMDRNGVPPSARGGPLPLEIMSGGGTHRPPRGPPSTSLGASRRPRARPPSEHNTEFLNVPDQGMAGMQRKRSVGQRPVPGVGRPKPQPRTHGPRCRALYQYVGQDVDELSFNVNEVIEILMEDPSGWWKGRLHGQEGLFPGNYVEKI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Horse
90
Bovine
90
Sheep
90
Chicken
90
Rabbit
90
Dog
89
Xenopus
80
Zebrafish
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00160 As Substrate

Site PTM Type Enzyme
Y65 Phosphorylation
S109 Phosphorylation
K116 Ubiquitination
K121 Acetylation
Y122 Phosphorylation
S131 Phosphorylation
K141 Ubiquitination
K158 Ubiquitination
K170 Ubiquitination
K194 Ubiquitination
S195 Phosphorylation
S299 Phosphorylation
K355 Ubiquitination
Y438 Phosphorylation
S535 Phosphorylation
T558 Phosphorylation
S561 Phosphorylation
K602 Ubiquitination
Y607 Phosphorylation
K611 Acetylation
K611 Ubiquitination
Y667 Phosphorylation
S671 Phosphorylation
K673 Ubiquitination
S734 Phosphorylation
Y771 Phosphorylation
Y790 Phosphorylation
K905 Ubiquitination
T910 Phosphorylation
T912 Phosphorylation
S922 Phosphorylation
S939 Phosphorylation
S973 Phosphorylation
S988 Phosphorylation
S992 Phosphorylation
S1001 Phosphorylation
S1023 Phosphorylation

Research Backgrounds

Function:

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments (By similarity).

Family&Domains:

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Restrictive clause

 

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