PSPC1 Antibody - #DF2220

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Product: PSPC1 Antibody
Catalog: DF2220
Description: Rabbit polyclonal antibody to PSPC1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
Mol.Wt.: 58 kDa; 59kD(Calculated).
Uniprot: Q8WXF1
RRID: AB_2839451

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(89%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
PSPC1 Antibody detects endogenous levels of total PSPC1.
RRID:
AB_2839451
Cite Format: Affinity Biosciences Cat# DF2220, RRID:AB_2839451.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Paraspeckle component 1; Paraspeckle protein 1; PSP1; Pspc1; PSPC1_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q8WXF1(PSPC1_HUMAN) >>Visit HPA database.

Gene(ID):
PSPC1(55269)
Expression:
Q8WXF1 PSPC1_HUMAN:

Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.

Description:
Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers.
Sequence:
MMLRGNLKQVRIEKNPARLRALESAVGESEPAAAAAMALALAGEPAPPAPAPPEDHPDEEMGFTIDIKSFLKPGEKTYTQRCRLFVGNLPTDITEEDFKRLFERYGEPSEVFINRDRGFGFIRLESRTLAEIAKAELDGTILKSRPLRIRFATHGAALTVKNLSPVVSNELLEQAFSQFGPVEKAVVVVDDRGRATGKGFVEFAAKPPARKALERCGDGAFLLTTTPRPVIVEPMEQFDDEDGLPEKLMQKTQQYHKEREQPPRFAQPGTFEFEYASRWKALDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRLEELRNQELQKRKQIQLRHEEEHRRREEEMIRHREQEELRRQQEGFKPNYMENREQEMRMGDMGPRGAINMGDAFSPAPAGNQGPPPMMGMNMNNRATIPGPPMGPGPAMGPEGAANMGTPMMPDNGAVHNDRFPQGPPSQMGSPMGSRTGSETPQAPMSGVGPVSGGPGGFGRGSQGGNFEGPNKRRRY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Chicken
100
Horse
92
Bovine
92
Sheep
92
Pig
89
Zebrafish
64
Dog
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WXF1 As Substrate

Site PTM Type Enzyme
M1 Acetylation
R4 Methylation
K8 Ubiquitination
K72 Ubiquitination
K76 Ubiquitination
K99 Acetylation
K134 Ubiquitination
K143 Ubiquitination
T159 Phosphorylation
S164 Phosphorylation
S168 Phosphorylation
T196 Phosphorylation
K198 Ubiquitination
K206 Acetylation
K251 Ubiquitination
Y255 Phosphorylation
Y275 Phosphorylation
K280 Ubiquitination
R338 Methylation
R373 Methylation
K380 Acetylation
S409 Phosphorylation
T453 Phosphorylation
S473 Phosphorylation
S477 Phosphorylation
S481 Phosphorylation
T487 Phosphorylation
S493 Phosphorylation
R507 Methylation
S509 Phosphorylation
K519 Acetylation
K519 Methylation
R520 Methylation

Research Backgrounds

Function:

Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity). Together with NONO, required for the formation of nuclear paraspeckles. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

Subcellular Location:

Nucleus>Nucleolus. Nucleus matrix. Cytoplasm. Nucleus speckle.
Note: In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in an RNA-dependent manner. May cycle between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.

Subunit Structure:

Forms heterodimers with NONO; this involves formation of a coiled coil domain by helices from both proteins. Found in a RNP complex with CAT2 transcribed nuclear RNA (CTN-RNA). Interaction with NONO is required for its targeting to paraspeckles and perinucleolar caps. Interacts with SFPQ (By similarity). Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA.

Family&Domains:

Belongs to the PSPC family.

References

1). Paraspeckles / CARM1 mediates the regulation of OEVs on cell differentiation during in vitro embryonic development of yak. Theriogenology, 2024 (PubMed: 38692037) [IF=2.4]

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