RFPL1 Antibody - #DF2231

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Product: RFPL1 Antibody
Catalog: DF2231
Description: Rabbit polyclonal antibody to RFPL1
Application: WB IHC
Reactivity: Human
Mol.Wt.: 35 kDa; 35kD(Calculated).
Uniprot: O75677
RRID: AB_2839462

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
RFPL1 Antibody detects endogenous levels of total RFPL1.
RRID:
AB_2839462
Cite Format: Affinity Biosciences Cat# DF2231, RRID:AB_2839462.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MGC132428; Ret finger protein-like 1; RFPL1; RFPL1_HUMAN; RING finger protein 78; RNF78;

Immunogens

Immunogen:
Uniprot:

O75677(RFPL1_HUMAN) >>Visit HPA database.

Gene(ID):
RFPL1(5988)
Expression:
O75677 RFPL1_HUMAN:

Seems to be expressed in prostate and less abundantly in adult brain, fetal liver, and fetal kidney.

Sequence:
MKRLSLVTTNRLSPHGNFLPLCTFPLAVDMAALFQEASSCPVCSDYLEKPMSLECGCAVCFKCINSLQKEPHGEDLLCCCCSMVSQKNKIRPSWQLERLASHIKELEPKLKKILQMNPRMRKFQVDMTLDADTANNFLLISDDLRSVRSGCITQNRQDLAERFDVSICILGSPRFTCGRHYWEVDVGTSTEWDLGVCRESVHRKGRIHLTTERGFWTVSLRDGSRLSASTVPLTFLFVDRKLQRVGIFLDMGMQNVSFFDAEGGSHVYTFRSVSAEEPLHLFFAPPSPPNGDKSVLSICPVINPGTTDAPVHPGEAK

PTMs - O75677 As Substrate

Site PTM Type Enzyme
S5 Phosphorylation

Research Backgrounds

Function:

Negatively regulates the G2-M phase transition, possibly by promoting cyclin B1/CCNB1 and CDK1 proteasomal degradation and thereby preventing their accumulation during interphase.

PTMs:

Phosphorylated by PKC and CDK1. The antiproliferative effect seems to be positively regulated by PKC phosphorylation and negatively by CDK1 phosphorylation.

Subcellular Location:

Cytoplasm. Nucleus.
Note: A higher concentration is observed in the cytoplasm compared to the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Seems to be expressed in prostate and less abundantly in adult brain, fetal liver, and fetal kidney.

Family&Domains:

The B30.2/SPRY domain is required for the negative regulation of cell proliferation.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.