PPP1R10 Antibody - #DF2235

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Product: PPP1R10 Antibody
Catalog: DF2235
Description: Rabbit polyclonal antibody to PPP1R10
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Dog
Mol.Wt.: 99 kDa; 99kD(Calculated).
Uniprot: Q96QC0
RRID: AB_2839466

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PPP1R10 Antibody detects endogenous levels of total PPP1R10.
RRID:
AB_2839466
Cite Format: Affinity Biosciences Cat# DF2235, RRID:AB_2839466.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CAT 53; CAT53; FB 19; FB19; FB19 protein; HLA-C associated transcript 53; MHC class I region proline rich protein CAT53; p99; Phosphatase 1 nuclear targeting subunit; Phosphatase nuclear targeting subunit; PNUTS; PP1 binding protein of 114 kDa; PP1 nuclear targeting subunit; PP1R10; protein FB19; Protein phosphatase 1 nuclear targeting subunit; Protein phosphatase 1 regulatory (inhibitor) subunit 10; Protein phosphatase 1 regulatory subunit 10; R111; Serine/threonine protein phosphatase 1 regulatory subunit 10;

Immunogens

Immunogen:
Uniprot:

Q96QC0(PP1RA_HUMAN) >>Visit HPA database.

Gene(ID):
PPP1R10(5514)
Expression:
Q96QC0 PP1RA_HUMAN:

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Sequence:
MGSGPIDPKELLKGLDSFLNRDGEVKSVDGISKIFSLMKEARKMVSRCTYLNILLQTRSPEILVKFIDVGGYKLLNNWLTYSKTTNNIPLLQQILLTLQHLPLTVDHLKQNNTAKLVKQLSKSSEDEELRKLASVLVSDWMAVIRSQSSTQPAEKDKKKRKDEGKSRTTLPERPLTEVKAETRAEEAPEKKREKPKSLRTTAPSHAKFRSTGLELETPSLVPVKKNASTVVVSDKYNLKPIPLKRQSNVAAPGDATPPAEKKYKPLNTTPNATKEIKVKIIPPQPMEGLGFLDALNSAPVPGIKIKKKKKVLSPTAAKPSPFEGKTSTEPSTAKPSSPEPAPPSEAMDADRPGTPVPPVEVPELMDTASLEPGALDAKPVESPGDPNQLTRKGRKRKSVTWPEEGKLREYFYFELDETERVNVNKIKDFGEAAKREILSDRHAFETARRLSHDNMEEKVPWVCPRPLVLPSPLVTPGSNSQERYIQAEREKGILQELFLNKESPHEPDPEPYEPIPPKLIPLDEECSMDETPYVETLEPGGSGGSPDGAGGSKLPPVLANLMGSMGAGKGPQGPGGGGINVQEILTSIMGSPNSHPSEELLKQPDYSDKIKQMLVPHGLLGPGPIANGFPPGGPGGPKGMQHFPPGPGGPMPGPHGGPGGPVGPRLLGPPPPPRGGDPFWDGPGDPMRGGPMRGGPGPGPGPYHRGRGGRGGNEPPPPPPPFRGARGGRSGGGPPNGRGGPGGGMVGGGGHRPHEGPGGGMGNSSGHRPHEGPGGGMGSGHRPHEGPGGSMGGGGGHRPHEGPGGGISGGSGHRPHEGPGGGMGAGGGHRPHEGPGGSMGGSGGHRPHEGPGHGGPHGHRPHDVPGHRGHDHRGPPPHEHRGHDGPGHGGGGHRGHDGGHSHGGDMSNRPVCRHFMMKGNCRYENNCAFYHPGVNGPPLP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
67
Zebrafish
67
Xenopus
60
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96QC0 As Substrate

Site PTM Type Enzyme
K13 Ubiquitination
S32 Phosphorylation
K33 Ubiquitination
K118 Ubiquitination
S134 Phosphorylation
S138 Phosphorylation
S149 Phosphorylation
T150 Phosphorylation
K207 Acetylation
K207 Ubiquitination
Y236 Phosphorylation
K239 Acetylation
K244 Acetylation
K244 Methylation
T256 Phosphorylation
K261 Acetylation
K262 Acetylation
Y263 Phosphorylation
T268 Phosphorylation
T269 Phosphorylation
K274 Acetylation
K274 Ubiquitination
S297 Phosphorylation
S313 Phosphorylation
T315 Phosphorylation
K318 Acetylation
S320 Phosphorylation
S331 Phosphorylation
T332 Phosphorylation
S336 Phosphorylation
S337 Phosphorylation
T354 Phosphorylation
S369 Phosphorylation
S382 Phosphorylation
T390 Phosphorylation
S398 Phosphorylation
T400 Phosphorylation
R441 Methylation
S451 Phosphorylation
S471 Phosphorylation
K491 Ubiquitination
K501 Ubiquitination
S503 Phosphorylation
S542 Phosphorylation
S545 Phosphorylation
S552 Phosphorylation
T586 Phosphorylation
S587 Phosphorylation
S591 Phosphorylation
S594 Phosphorylation
S597 Phosphorylation
K602 Ubiquitination
K609 Ubiquitination
R665 Methylation
R674 Methylation
R688 Methylation
R693 Methylation
R705 Methylation
R707 Methylation
R710 Methylation
R723 Methylation
R726 Methylation
R729 Methylation
S779 Phosphorylation
S790 Phosphorylation

Research Backgrounds

Function:

Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers (By similarity).

PTMs:

Phosphorylated on Ser-398 by PKA within the region necessary for interaction with PPP1CA.

Subcellular Location:

Nucleus.
Note: Found in discrete nucleoplasmic bodies and within nucleoli. Associates with chromatin during interphase, excluded from condensed chromosomes during early mitosis and is reloaded onto chromosomes at the late telophase (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Subunit Structure:

Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA or PPP1CB or PPP1CC. Interacts with PPP1CC. Interacts with PPP1CA, WDR82 and TOX4 (By similarity).

Restrictive clause

 

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For Research Use Only.
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