Product: TNNT3 Antibody
Catalog: DF2256
Description: Rabbit polyclonal antibody to TNNT3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Dog, Chicken
Mol.Wt.: 32 kDa; 32kD(Calculated).
Uniprot: P45378
RRID: AB_2839485

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(92%), Bovine(92%), Horse(92%), Dog(92%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
TNNT3 Antibody detects endogenous levels of total TNNT3.
RRID:
AB_2839485
Cite Format: Affinity Biosciences Cat# DF2256, RRID:AB_2839485.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AMCD2B; Beta TnTF; Beta-TnTF; DA2B; DKFZp779M2348; fast skeletal muscle; Fast skeletal muscle troponin T; FSSV; fTnT; TNNT 3; Tnnt3; TNNT3_HUMAN; TnTf; Troponin T; Troponin T fast skeletal muscle; Troponin T type 3 (skeletal fast); Troponin T type 3; Troponin T3 skeletal fast;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P45378 TNNT3_HUMAN:

In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle.

Description:
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Sequence:
MSDEEVEQVEEQYEEEEEAQEEAAEVHEEVHEPEEVQEDTAEEDAEEEKPRPKLTAPKIPEGEKVDFDDIQKKRQNKDLMELQALIDSHFEARKKEEEELVALKERIEKRRAERAEQQRIRAEKERERQNRLAEEKARREEEDAKRRAEDDLKKKKALSSMGANYSSYLAKADQKRGKKQTAREMKKKILAERRKPLNIDHLGEDKLRDKAKELWETLHQLEIDKFEFGEKLKRQKYDITTLRSRIDQAQKHSKKAGTPAKGKVGGRWK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Bovine
92
Dog
92
Chicken
92
Sheep
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P45378 As Substrate

Site PTM Type Enzyme
R119 Methylation
S159 Phosphorylation
Y165 Phosphorylation
S166 Phosphorylation
S167 Phosphorylation
Y168 Phosphorylation
K254 Acetylation
K261 Acetylation
K263 Acetylation

Research Backgrounds

Function:

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Tissue Specificity:

In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle.

Family&Domains:

Belongs to the troponin T family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.