USP37 Antibody - #DF2259

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Product: USP37 Antibody
Catalog: DF2259
Description: Rabbit polyclonal antibody to USP37
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 120 kDa; 110kD(Calculated).
Uniprot: Q86T82
RRID: AB_2839488

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
USP37 Antibody detects endogenous levels of total USP37.
RRID:
AB_2839488
Cite Format: Affinity Biosciences Cat# DF2259, RRID:AB_2839488.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Deubiquitinating enzyme 3; Deubiquitinating enzyme 37; EC 3.1.2.15; KIAA1594; MGC117261; OTTHUMP00000164146; tmp_locus_50; Ubiquitin carboxyl-terminal hydrolase 37; Ubiquitin specific peptidase 37; Ubiquitin specific processing protease 37; Ubiquitin specific protease 37; Ubiquitin thioesterase 37; Ubiquitin thiolesterase 37; Ubiquitin-specific-processing protease 37; UBP37_HUMAN; USP37;

Immunogens

Immunogen:
Uniprot:

Q86T82(UBP37_HUMAN) >>Visit HPA database.

Gene(ID):
USP37(57695)
Expression:
Q86T82 UBP37_HUMAN:

Expressed in brain and prostate.

Sequence:
MSPLKIHGPIRIRSMQTGITKWKEGSFEIVEKENKVSLVVHYNTGGIPRIFQLSHNIKNVVLRPSGAKQSRLMLTLQDNSFLSIDKVPSKDAEEMRLFLDAVHQNRLPAAMKPSQGSGSFGAILGSRTSQKETSRQLSYSDNQASAKRGSLETKDDIPFRKVLGNPGRGSIKTVAGSGIARTIPSLTSTSTPLRSGLLENRTEKRKRMISTGSELNEDYPKENDSSSNNKAMTDPSRKYLTSSREKQLSLKQSEENRTSGLLPLQSSSFYGSRAGSKEHSSGGTNLDRTNVSSQTPSAKRSLGFLPQPVPLSVKKLRCNQDYTGWNKPRVPLSSHQQQQLQGFSNLGNTCYMNAILQSLFSLQSFANDLLKQGIPWKKIPLNALIRRFAHLLVKKDICNSETKKDLLKKVKNAISATAERFSGYMQNDAHEFLSQCLDQLKEDMEKLNKTWKTEPVSGEENSPDISATRAYTCPVITNLEFEVQHSIICKACGEIIPKREQFNDLSIDLPRRKKPLPPRSIQDSLDLFFRAEELEYSCEKCGGKCALVRHKFNRLPRVLILHLKRYSFNVALSLNNKIGQQVIIPRYLTLSSHCTENTKPPFTLGWSAHMAISRPLKASQMVNSCITSPSTPSKKFTFKSKSSLALCLDSDSEDELKRSVALSQRLCEMLGNEQQQEDLEKDSKLCPIEPDKSELENSGFDRMSEEELLAAVLEISKRDASPSLSHEDDDKPTSSPDTGFAEDDIQEMPENPDTMETEKPKTITELDPASFTEITKDCDENKENKTPEGSQGEVDWLQQYDMEREREEQELQQALAQSLQEQEAWEQKEDDDLKRATELSLQEFNNSFVDALGSDEDSGNEDVFDMEYTEAEAEELKRNAETGNLPHSYRLISVVSHIGSTSSSGHYISDVYDIKKQAWFTYNDLEVSKIQEAAVQSDRDRSGYIFFYMHKEIFDELLETEKNSQSLSTEVGKTTRQAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
91
Zebrafish
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86T82 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
K68 Ubiquitination
S80 Phosphorylation
S83 Phosphorylation
S114 Phosphorylation
S138 Phosphorylation
K147 Ubiquitination
S150 Phosphorylation
K161 Ubiquitination
R168 Methylation
S170 Phosphorylation
T191 Phosphorylation
S210 Phosphorylation
T211 Phosphorylation
S213 Phosphorylation
Y219 Phosphorylation
S227 Phosphorylation
K246 Ubiquitination
K251 Ubiquitination
S268 Phosphorylation
K277 Ubiquitination
R288 Methylation
K299 Ubiquitination
S301 Phosphorylation
S312 Phosphorylation
K327 Ubiquitination
K411 Ubiquitination
S415 Phosphorylation
T417 Phosphorylation
K446 Acetylation
K452 Sumoylation
K452 Ubiquitination
S457 Phosphorylation
S462 Phosphorylation
K498 Ubiquitination
K544 Ubiquitination
S573 Phosphorylation
S624 Phosphorylation
T627 Phosphorylation
S628 Phosphorylation P24941 (CDK2)
T631 Phosphorylation
S650 Phosphorylation
S652 Phosphorylation
K681 Ubiquitination
S683 Phosphorylation
K684 Ubiquitination
K692 Ubiquitination
S698 Phosphorylation
K717 Ubiquitination
S770 Phosphorylation
K776 Ubiquitination
K785 Ubiquitination
K916 Ubiquitination
K962 Ubiquitination
K973 Ubiquitination

Research Backgrounds

Function:

Deubiquitinase that antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Also mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains in vitro. Phosphorylation at Ser-628 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2). Plays an important role in the regulation of DNA replication by stabilizing the licensing factor CDT1.

PTMs:

Polyubiquitinated via 'Lys-11'-linked ubiquitin by the APC(CDH1) complex during late mitosis, leading to its degradation. Able to mediate auto-deubiquitination.

Phosphorylated at Ser-628 by CDK2 during G1/S phase but not during mitosis; phosphorylation at Ser-628 is required for deubiquitinase activity. Also polyubiquitinated during early G1 phase, without leading to degradation.

Tissue Specificity:

Expressed in brain and prostate.

Subunit Structure:

Interacts with FZR1/CDH1. Interacts with CDT1.

Family&Domains:

The KEN box 3 is required for interaction with FZR1/CDH1 and is essential for APC(CDH1)-mediated ubiquitination.

Belongs to the peptidase C19 family.

Restrictive clause

 

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