ASPP1 Antibody - #DF2274

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Product: ASPP1 Antibody
Catalog: DF2274
Description: Rabbit polyclonal antibody to ASPP1
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 120 kDa; 120kD(Calculated).
Uniprot: Q96KQ4
RRID: AB_2839503

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
ASPP1 Antibody detects endogenous levels of total ASPP1.
RRID:
AB_2839503
Cite Format: Affinity Biosciences Cat# DF2274, RRID:AB_2839503.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Apoptosis-stimulating of p53 protein 1; ASPP1_HUMAN; p53BP2 like; p85; Ppp1r13b; Protein phosphatase 1 regulatory subunit 13B;

Immunogens

Immunogen:
Uniprot:

Q96KQ4(ASPP1_HUMAN) >>Visit HPA database.

Gene(ID):
PPP1R13B(23368)
Expression:
Q96KQ4 ASPP1_HUMAN:

Reduced expression in breast carcinomas expressing a wild-type TP53 protein.

Description:
Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo
Sequence:
MMPMILTVFLSNNEQILTEVPITPETTCRDVVEFCKEPGEGSCHLAEVWRGNERPIPFDHMMYEHLQKWGPRREEVKFFLRHEDSPTENSEQGGRQTQEQRTQRNVINVPGEKRTENGVGNPRVELTLSELQDMAARQQQQIENQQQMLVAKEQRLHFLKQQERRQQQSISENEKLQKLKERVEAQENKLKKIRAMRGQVDYSKIMNGNLSAEIERFSAMFQEKKQEVQTAILRVDQLSQQLEDLKKGKLNGFQSYNGKLTGPAAVELKRLYQELQIRNQLNQEQNSKLQQQKELLNKRNMEVAMMDKRISELRERLYGKKIQLNRVNGTSSPQSPLSTSGRVAAVGPYIQVPSAGSFPVLGDPIKPQSLSIASNAAHGRSKSANDGNWPTLKQNSSSSVKPVQVAGADWKDPSVEGSVKQGTVSSQPVPFSALGPTEKPGIEIGKVPPPIPGVGKQLPPSYGTYPSPTPLGPGSTSSLERRKEGSLPRPSAGLPSRQRPTLLPATGSTPQPGSSQQIQQRISVPPSPTYPPAGPPAFPAGDSKPELPLTVAIRPFLADKGSRPQSPRKGPQTVNSSSIYSMYLQQATPPKNYQPAAHSALNKSVKAVYGKPVLPSGSTSPSPLPFLHGSLSTGTPQPQPPSESTEKEPEQDGPAAPADGSTVESLPRPLSPTKLTPIVHSPLRYQSDADLEALRRKLANAPRPLKKRSSITEPEGPGGPNIQKLLYQRFNTLAGGMEGTPFYQPSPSQDFMGTLADVDNGNTNANGNLEELPPAQPTAPLPAEPAPSSDANDNELPSPEPEELICPQTTHQTAEPAEDNNNNVATVPTTEQIPSPVAEAPSPGEEQVPPAPLPPASHPPATSTNKRTNLKKPNSERTGHGLRVRFNPLALLLDASLEGEFDLVQRIIYEVEDPSKPNDEGITPLHNAVCAGHHHIVKFLLDFGVNVNAADSDGWTPLHCAASCNSVHLCKQLVESGAAIFASTISDIETAADKCEEMEEGYIQCSQFLYGVQEKLGVMNKGVAYALWDYEAQNSDELSFHEGDALTILRRKDESETEWWWARLGDREGYVPKNLLGLYPRIKPRQRTLA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Chicken
100
Rabbit
100
Pig
92
Bovine
92
Sheep
92
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96KQ4 As Substrate

Site PTM Type Enzyme
K113 Ubiquitination
K160 Ubiquitination
S169 Phosphorylation
T230 Phosphorylation
K246 Ubiquitination
K269 Ubiquitination
K288 Ubiquitination
K293 Ubiquitination
S311 Phosphorylation
T330 Phosphorylation
S332 Phosphorylation
S335 Phosphorylation
Y349 Phosphorylation
K382 Methylation
K393 Methylation
S414 Phosphorylation
S486 Phosphorylation
R499 Methylation
R521 Methylation
S566 Phosphorylation
S620 Phosphorylation
S630 Phosphorylation
T635 Phosphorylation
S671 Phosphorylation
T673 Phosphorylation
S681 Phosphorylation
Y685 Phosphorylation
S687 Phosphorylation
K707 Methylation
S709 Phosphorylation
S710 Phosphorylation
Y727 Phosphorylation

Research Backgrounds

Function:

Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Predominantly cytoplasmic. Some fraction is nuclear.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Reduced expression in breast carcinomas expressing a wild-type TP53 protein.

Subunit Structure:

Interacts with P53/TP53; the interaction promotes pro-apototic activity.

Family&Domains:

The ankyrin repeats and the SH3 domain are required for specific interactions with TP53.

Belongs to the ASPP family.

Restrictive clause

 

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