CDH12 Antibody - #DF2278

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Product: CDH12 Antibody
Catalog: DF2278
Description: Rabbit polyclonal antibody to CDH12
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 88 kDa; 88kD(Calculated).
Uniprot: P55289
RRID: AB_2839506

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
CDH12 Antibody detects endogenous levels of total CDH12.
RRID:
AB_2839506
Cite Format: Affinity Biosciences Cat# DF2278, RRID:AB_2839506.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Br cadherin; BR-cadherin; Brain cadherin; CAD12_HUMAN; cadherin 12 (N cadherin 2); Cadherin 12; Cadherin 12 precursor; Cadherin 12 type 2 (N cadherin 2); Cadherin 12 type 2; Cadherin neural type 2; Cadherin-12; CDH12; CDHB; FLJ34857; N cadherin 2; N-cadherin 2; Neural type cadherin 2; Neuronal cadherin 2;

Immunogens

Immunogen:
Uniprot:

P55289(CAD12_HUMAN) >>Visit HPA database.

Gene(ID):
CDH12(1010)
Expression:
P55289 CAD12_HUMAN:

Brain.

Description:
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types
Sequence:
MLTRNCLSLLLWVLFDGGLLTPLQPQPQQTLATEPRENVIHLPGQRSHFQRVKRGWVWNQFFVLEEYVGSEPQYVGKLHSDLDKGEGTVKYTLSGDGAGTVFTIDETTGDIHAIRSLDREEKPFYTLRAQAVDIETRKPLEPESEFIIKVQDINDNEPKFLDGPYVATVPEMSPVGAYVLQVKATDADDPTYGNSARVVYSILQGQPYFSIDPKTGVIRTALPNMDREVKEQYQVLIQAKDMGGQLGGLAGTTIVNITLTDVNDNPPRFPKSIFHLKVPESSPIGSAIGRIRAVDPDFGQNAEIEYNIVPGDGGNLFDIVTDEDTQEGVIKLKKPLDFETKKAYTFKVEASNLHLDHRFHSAGPFKDTATVKISVLDVDEPPVFSKPLYTMEVYEDTPVGTIIGAVTAQDLDVGSSAVRYFIDWKSDGDSYFTIDGNEGTIATNELLDRESTAQYNFSIIASKVSNPLLTSKVNILINVLDVNEFPPEISVPYETAVCENAKPGQIIQIVSAADRDLSPAGQQFSFRLSPEAAIKPNFTVRDFRNNTAGIETRRNGYSRRQQELYFLPVVIEDSSYPVQSSTNTMTIRVCRCDSDGTILSCNVEAIFLPVGLSTGALIAILLCIVILLAIVVLYVALRRQKKKDTLMTSKEDIRDNVIHYDDEGGGEEDTQAFDIGALRNPKVIEENKIRRDIKPDSLCLPRQRPPMEDNTDIRDFIHQRLQENDVDPTAPPYDSLATYAYEGSGSVAESLSSIDSLTTEADQDYDYLTDWGPRFKVLADMFGEEESYNPDKVT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Pig
91
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55289 As Substrate

Site PTM Type Enzyme
Y233 Phosphorylation
S286 Phosphorylation
T340 Phosphorylation
K641 Methylation
K642 Methylation

Research Backgrounds

Function:

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain.

Family&Domains:

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.

References

1). Cadherin-12 Regulates Neurite Outgrowth Through the PKA/Rac1/Cdc42 Pathway in Cortical Neurons. Frontiers in Cell and Developmental Biology (PubMed: 34820384) [IF=5.5]

Application: IF/ICC    Species: zebrafish    Sample:

FIGURE 1 CDH12 knockdown decreased neurite outgrowth in E18 neurons. (A) Representative images of immunofluorescence staining showing the expression of CDH12 in the cell membrane of the cell body, axon, and growth cone. The white dashed box shows an enlarged view of the growth cone. The scale bar represents 25 μm. (B) The mRNA level of CDH12 in cortical neurons decreased gradually with development (E18, P1, P3) as detected using real-time PCR. The level of CDH12 mRNA in E18 neurons was normalized as 1. The data were shown as mean ± SE, n = 3, ∗∗∗P < 0.001. Western blotting (C) and statistical (D) results showing the effectiveness of small interfering RNA in knocking down CDH12 expression. β-actin was used as a loading control. The value of CDH12/β-actin in NC siRNA treatment was normalized as 1. The data were shown as mean ± SE, n = 3, ∗P < 0.05. (E,F) CDH12 knockdown significantly decreased axon growth in E18 neurons after CDH12 siRNA treatment for 36 h. The scale bar represents 75 μm. The data were shown as mean ± SD, n = 96, ∗∗∗P < 0.001. (G) The ratio of neuronal development (with different axonal lengths) was affected by CDH12 knockdown. n = 100, ∗∗∗P < 0.001 (two-way ANOVA).

Application: WB    Species: zebrafish    Sample:

FIGURE 1 CDH12 knockdown decreased neurite outgrowth in E18 neurons. (A) Representative images of immunofluorescence staining showing the expression of CDH12 in the cell membrane of the cell body, axon, and growth cone. The white dashed box shows an enlarged view of the growth cone. The scale bar represents 25 μm. (B) The mRNA level of CDH12 in cortical neurons decreased gradually with development (E18, P1, P3) as detected using real-time PCR. The level of CDH12 mRNA in E18 neurons was normalized as 1. The data were shown as mean ± SE, n = 3, ∗∗∗P < 0.001. Western blotting (C) and statistical (D) results showing the effectiveness of small interfering RNA in knocking down CDH12 expression. β-actin was used as a loading control. The value of CDH12/β-actin in NC siRNA treatment was normalized as 1. The data were shown as mean ± SE, n = 3, ∗P < 0.05. (E,F) CDH12 knockdown significantly decreased axon growth in E18 neurons after CDH12 siRNA treatment for 36 h. The scale bar represents 75 μm. The data were shown as mean ± SD, n = 96, ∗∗∗P < 0.001. (G) The ratio of neuronal development (with different axonal lengths) was affected by CDH12 knockdown. n = 100, ∗∗∗P < 0.001 (two-way ANOVA).

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