CCNY Antibody - #DF2290

(2)  
Product: CCNY Antibody
Catalog: DF2290
Description: Rabbit polyclonal antibody to CCNY
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 39 kDa; 39kD(Calculated).
Uniprot: Q8ND76
RRID: AB_2839516

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
CCNY Antibody detects endogenous levels of total CCNY.
RRID:
AB_2839516
Cite Format: Affinity Biosciences Cat# DF2290, RRID:AB_2839516.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C10orf9; CBCP1; CCNX; CCNY; CFP1; Chromosome 10 open reading frame 9; Cyclin box carrying protein 1; Cyclin box protein 1; Cyclin fold protein 1; Cyclin X; Cyclin Y;

Immunogens

Immunogen:
Uniprot:

Q8ND76(CCNY_HUMAN) >>Visit HPA database.

Gene(ID):
CCNY(219771)
Expression:
Q8ND76 CCNY_HUMAN:

Widely expressed.

Sequence:
MGNTTSCCVSSSPKLRRNAHSRLESYRPDTDLSREDTGCNLQHISDRENIDDLNMEFNPSDHPRASTIFLSKSQTDVREKRKSLFINHHPPGQIARKYSSCSTIFLDDSTVSQPNLKYTIKCVALAIYYHIKNRDPDGRMLLDIFDENLHPLSKSEVPPDYDKHNPEQKQIYRFVRTLFSAAQLTAECAIVTLVYLERLLTYAEIDICPANWKRIVLGAILLASKVWDDQAVWNVDYCQILKDITVEDMNELERQFLELLQFNINVPSSVYAKYYFDLRSLAEANNLSFPLEPLSRERAHKLEAISRLCEDKYKDLRRSARKRSASADNLTLPRWSPAIIS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Zebrafish
92
Rabbit
92
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8ND76 As Substrate

Site PTM Type Enzyme
G2 Myristoylation
S12 Phosphorylation
S21 Phosphorylation
S25 Phosphorylation
Y26 Phosphorylation
T30 Phosphorylation
S33 Phosphorylation O94921 (CDK14)
T37 Phosphorylation O94921 (CDK14)
S66 Phosphorylation
T67 Phosphorylation
S71 Phosphorylation
K72 Ubiquitination
S73 Phosphorylation
T75 Phosphorylation
S83 Phosphorylation
Y98 Phosphorylation
S99 Phosphorylation
S100 Phosphorylation
S102 Phosphorylation
T103 Phosphorylation
S153 Phosphorylation
Y237 Phosphorylation
S280 Phosphorylation
S288 Phosphorylation
S295 Phosphorylation
K301 Ubiquitination
S324 Phosphorylation
S326 Phosphorylation
T331 Phosphorylation
S336 Phosphorylation Q00536 (CDK16)

Research Backgrounds

Function:

Positive regulatory subunit of the cyclin-dependent kinases CDK14/PFTK1 and CDK16. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway. Recruits CDK16 to the plasma membrane. Isoform 3 might play a role in the activation of MYC-mediated transcription.

PTMs:

Ubiquitinated; leading to its degradation.

Heavily phosphorylated. Phosphorylation at Ser-71 and Ser-73 by CDK14 is enhanced during the G2 and M cell cycle phases, and creates a phosphodegron triggering SCF-dependent ubiquitination.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side.

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Found in a complex with CAPRIN2, LRP6 AND CDK14 during G2/M stage; CAPRIN2 functions as a scaffold for the complex by binding to CCNY via its N terminus and to CDK14 via its C terminus. Interacts with CDK14. Interacts with CDK16. Interacts with LRP6.

Family&Domains:

Belongs to the cyclin family. Cyclin Y subfamily.

Restrictive clause

 

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