CENPF Antibody | Affinity Biosciences

WB
IHC
Cites

1/4

CENPF Antibody - Western blot analysis of extracts from A549 cells, using CENPF Antibody.

CENPF Antibody - DF2310 at 1/100 staining Mouse kidney tissue by IHC-P.

CENPF Antibody - Figure 5.

CENPF Antibody - Figure 3 CENPF regulates ADR-induced G2/M phase arrest through Chk1.

Product:	CENPF Antibody
Catalog:	DF2310
Description:	Rabbit polyclonal antibody to CENPF
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Pig
Mol.Wt.:	367 kDa; 358kD(Calculated).
Uniprot:	P49454
RRID:	AB_2839534

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(82%)

Clonality:

Polyclonal

Specificity:

CENPF Antibody detects endogenous levels of total CENPF.

RRID:

AB_2839534
Cite Format: Affinity Biosciences Cat# DF2310, RRID:AB_2839534.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AH antigen; Cell cycle dependent 350K nuclear protein; CENF; CENP F; CENP F kinetochore protein; CENP-F; CENPF; CENPF kinetochore protein; CENPF_HUMAN; Centromere protein F 350/400ka; Centromere protein F; Centromere protein F, 350/400kDa; CILD31; Hcp 1; Hcp1; Kinetochore protein CENP F; Kinetochore protein CENPF; Mitosin; PRO1779; STROMS;

Immunogens

Immunogen:

Uniprot:

P49454(CENPF_HUMAN) >>Visit HPA database.

Gene(ID):

CENPF(1063)

Description:

Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia

Sequence:

P49454 CENPF_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSWALEEWKEGLPTRALQKIQELEGQLDKLKKEKQQRQFQLDSLEAALQKQKQKVENEKTEGTNLKRENQRLMEICESLEKTKQKISHELQVKESQVNFQEGQLNSGKKQIEKLEQELKRCKSELERSQQAAQSADVSLNPCNTPQKIFTTPLTPSQYYSGSKYEDLKEKYNKEVEERKRLEAEVKALQAKKASQTLPQATMNHRDIARHQASSSVFSWQQEKTPSHLSSNSQRTPIRRDFSASYFSGEQEVTPSRSTLQIGKRDANSSFFDNSSSPHLLDQLKAQNQELRNKINELELRLQGHEKEMKGQVNKFQELQLQLEKAKVELIEKEKVLNKCRDELVRTTAQYDQASTKYTALEQKLKKLTEDLSCQRQNAESARCSLEQKIKEKEKEFQEELSRQQRSFQTLDQECIQMKARLTQELQQAKNMHNVLQAELDKLTSVKQQLENNLEEFKQKLCRAEQAFQASQIKENELRRSMEEMKKENNLLKSHSEQKAREVCHLEAELKNIKQCLNQSQNFAEEMKAKNTSQETMLRDLQEKINQQENSLTLEKLKLAVADLEKQRDCSQDLLKKREHHIEQLNDKLSKTEKESKALLSALELKKKEYEELKEEKTLFSCWKSENEKLLTQMESEKENLQSKINHLETCLKTQQIKSHEYNERVRTLEMDRENLSVEIRNLHNVLDSKSVEVETQKLAYMELQQKAEFSDQKHQKEIENMCLKTSQLTGQVEDLEHKLQLLSNEIMDKDRCYQDLHAEYESLRDLLKSKDASLVTNEDHQRSLLAFDQQPAMHHSFANIIGEQGSMPSERSECRLEADQSPKNSAILQNRVDSLEFSLESQKQMNSDLQKQCEELVQIKGEIEENLMKAEQMHQSFVAETSQRISKLQEDTSAHQNVVAETLSALENKEKELQLLNDKVETEQAEIQELKKSNHLLEDSLKELQLLSETLSLEKKEMSSIISLNKREIEELTQENGTLKEINASLNQEKMNLIQKSESFANYIDEREKSISELSDQYKQEKLILLQRCEETGNAYEDLSQKYKAAQEKNSKLECLLNECTSLCENRKNELEQLKEAFAKEHQEFLTKLAFAEERNQNLMLELETVQQALRSEMTDNQNNSKSEAGGLKQEIMTLKEEQNKMQKEVNDLLQENEQLMKVMKTKHECQNLESEPIRNSVKERESERNQCNFKPQMDLEVKEISLDSYNAQLVQLEAMLRNKELKLQESEKEKECLQHELQTIRGDLETSNLQDMQSQEISGLKDCEIDAEEKYISGPHELSTSQNDNAHLQCSLQTTMNKLNELEKICEILQAEKYELVTELNDSRSECITATRKMAEEVGKLLNEVKILNDDSGLLHGELVEDIPGGEFGEQPNEQHPVSLAPLDESNSYEHLTLSDKEVQMHFAELQEKFLSLQSEHKILHDQHCQMSSKMSELQTYVDSLKAENLVLSTNLRNFQGDLVKEMQLGLEEGLVPSLSSSCVPDSSSLSSLGDSSFYRALLEQTGDMSLLSNLEGAVSANQCSVDEVFCSSLQEENLTRKETPSAPAKGVEELESLCEVYRQSLEKLEEKMESQGIMKNKEIQELEQLLSSERQELDCLRKQYLSENEQWQQKLTSVTLEMESKLAAEKKQTEQLSLELEVARLQLQGLDLSSRSLLGIDTEDAIQGRNESCDISKEHTSETTERTPKHDVHQICDKDAQQDLNLDIEKITETGAVKPTGECSGEQSPDTNYEPPGEDKTQGSSECISELSFSGPNALVPMDFLGNQEDIHNLQLRVKETSNENLRLLHVIEDRDRKVESLLNEMKELDSKLHLQEVQLMTKIEACIELEKIVGELKKENSDLSEKLEYFSCDHQELLQRVETSEGLNSDLEMHADKSSREDIGDNVAKVNDSWKERFLDVENELSRIRSEKASIEHEALYLEADLEVVQTEKLCLEKDNENKQKVIVCLEEELSVVTSERNQLRGELDTMSKKTTALDQLSEKMKEKTQELESHQSECLHCIQVAEAEVKEKTELLQTLSSDVSELLKDKTHLQEKLQSLEKDSQALSLTKCELENQIAQLNKEKELLVKESESLQARLSESDYEKLNVSKALEAALVEKGEFALRLSSTQEEVHQLRRGIEKLRVRIEADEKKQLHIAEKLKERERENDSLKDKVENLERELQMSEENQELVILDAENSKAEVETLKTQIEEMARSLKVFELDLVTLRSEKENLTKQIQEKQGQLSELDKLLSSFKSLLEEKEQAEIQIKEESKTAVEMLQNQLKELNEAVAALCGDQEIMKATEQSLDPPIEEEHQLRNSIEKLRARLEADEKKQLCVLQQLKESEHHADLLKGRVENLERELEIARTNQEHAALEAENSKGEVETLKAKIEGMTQSLRGLELDVVTIRSEKENLTNELQKEQERISELEIINSSFENILQEKEQEKVQMKEKSSTAMEMLQTQLKELNERVAALHNDQEACKAKEQNLSSQVECLELEKAQLLQGLDEAKNNYIVLQSSVNGLIQEVEDGKQKLEKKDEEISRLKNQIQDQEQLVSKLSQVEGEHQLWKEQNLELRNLTVELEQKIQVLQSKNASLQDTLEVLQSSYKNLENELELTKMDKMSFVEKVNKMTAKETELQREMHEMAQKTAELQEELSGEKNRLAGELQLLLEEIKSSKDQLKELTLENSELKKSLDCMHKDQVEKEGKVREEIAEYQLRLHEAEKKHQALLLDTNKQYEVEIQTYREKLTSKEECLSSQKLEIDLLKSSKEELNNSLKATTQILEELKKTKMDNLKYVNQLKKENERAQGKMKLLIKSCKQLEEEKEILQKELSQLQAAQEKQKTGTVMDTKVDELTTEIKELKETLEEKTKEADEYLDKYCSLLISHEKLEKAKEMLETQVAHLCSQQSKQDSRGSPLLGPVVPGPSPIPSVTEKRLSSGQNKASGKRQRSSGIWENGRGPTPATPESFSKKSKKAVMSGIHPAEDTEGTEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRLAAQKLALSPLSLGKENLAESSKPTAGGSRSQKVKVAQRSPVDSGTILREPTTKSVPVNNLPERSPTDSPREGLRVKRGRLVPSPKAGLESNGSENCKVQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Sheep

Horse

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49454 As Substrate

P49454

Site	PTM Type	Enzyme	Source
K19	Ubiquitination		Uniprot
K29	Ubiquitination		Uniprot
K50	Ubiquitination		Uniprot
S78	Phosphorylation		Uniprot
K93	Ubiquitination		Uniprot
S106	Phosphorylation		Uniprot
K113	Ubiquitination		Uniprot
S138	Phosphorylation		Uniprot
T144	Phosphorylation		Uniprot
K147	Ubiquitination		Uniprot
T150	Phosphorylation		Uniprot
T151	Phosphorylation		Uniprot
T154	Phosphorylation	P24941 (CDK2)	Uniprot
S156	Phosphorylation	P24941 (CDK2)	Uniprot
Y158	Phosphorylation		Uniprot
Y159	Phosphorylation		Uniprot
S160	Phosphorylation		Uniprot
S162	Phosphorylation		Uniprot
K163	Ubiquitination		Uniprot
K186	Ubiquitination		Uniprot
K192	Ubiquitination		Uniprot
K223	Ubiquitination		Uniprot
T224	Phosphorylation		Uniprot
T235	Phosphorylation		Uniprot
S242	Phosphorylation		Uniprot
S247	Phosphorylation		Uniprot
T253	Phosphorylation		Uniprot
S255	Phosphorylation		Uniprot
S257	Phosphorylation		Uniprot
K263	Ubiquitination		Uniprot
S268	Phosphorylation		Uniprot
S269	Phosphorylation		Uniprot
S274	Phosphorylation		Uniprot
S275	Phosphorylation		Uniprot
S276	Phosphorylation		Uniprot
K284	Ubiquitination		Uniprot
K293	Ubiquitination		Uniprot
K306	Ubiquitination		Uniprot
K314	Ubiquitination		Uniprot
K324	Ubiquitination		Uniprot
K356	Ubiquitination		Uniprot
K363	Ubiquitination		Uniprot
K366	Ubiquitination		Uniprot
S372	Phosphorylation		Uniprot
K388	Ubiquitination		Uniprot
K394	Ubiquitination		Uniprot
K418	Ubiquitination		Uniprot
T422	Phosphorylation		Uniprot
K429	Ubiquitination		Uniprot
K446	Ubiquitination		Uniprot
K457	Ubiquitination		Uniprot
K473	Ubiquitination		Uniprot
S480	Phosphorylation		Uniprot
K486	Ubiquitination		Uniprot
K492	Ubiquitination		Uniprot
K498	Ubiquitination		Uniprot
K510	Ubiquitination		Uniprot
K513	Ubiquitination		Uniprot
K527	Ubiquitination		Uniprot
K529	Ubiquitination		Uniprot
K543	Acetylation		Uniprot
K543	Ubiquitination		Uniprot
K555	Ubiquitination		Uniprot
K557	Ubiquitination		Uniprot
K565	Ubiquitination		Uniprot
K576	Ubiquitination		Uniprot
K587	Ubiquitination		Uniprot
K596	Ubiquitination		Uniprot
K605	Ubiquitination		Uniprot
K616	Ubiquitination		Uniprot
K628	Ubiquitination		Uniprot
K637	Ubiquitination		Uniprot
K643	Ubiquitination		Uniprot
K652	Ubiquitination		Uniprot
K657	Ubiquitination		Uniprot
S676	Phosphorylation		Uniprot
K689	Ubiquitination		Uniprot
K697	Ubiquitination		Uniprot
Y700	Phosphorylation		Uniprot
K706	Ubiquitination		Uniprot
K738	Ubiquitination		Uniprot
S743	Phosphorylation		Uniprot
K749	Ubiquitination		Uniprot
K768	Ubiquitination		Uniprot
K770	Ubiquitination		Uniprot
S773	Phosphorylation		Uniprot
S783	Phosphorylation		Uniprot
S796	Phosphorylation		Uniprot
S809	Phosphorylation		Uniprot
S821	Phosphorylation		Uniprot
K823	Ubiquitination		Uniprot
S825	Phosphorylation		Uniprot
S834	Phosphorylation		Uniprot
S838	Phosphorylation		Uniprot
S876	Phosphorylation		Uniprot
K887	Ubiquitination		Uniprot
K909	Ubiquitination		Uniprot
K911	Ubiquitination		Uniprot
K919	Ubiquitination		Uniprot
K931	Ubiquitination		Uniprot
K932	Ubiquitination		Uniprot
S933	Phosphorylation		Uniprot
S940	Phosphorylation		Uniprot
K942	Ubiquitination		Uniprot
K955	Ubiquitination		Uniprot
K956	Ubiquitination		Uniprot
S963	Phosphorylation		Uniprot
T973	Phosphorylation		Uniprot
K980	Ubiquitination		Uniprot
S985	Phosphorylation		Uniprot
K990	Ubiquitination		Uniprot
K996	Ubiquitination		Uniprot
K1009	Ubiquitination		Uniprot
S1010	Phosphorylation		Uniprot
K1019	Ubiquitination		Uniprot
K1022	Ubiquitination		Uniprot
K1042	Ubiquitination		Uniprot
K1052	Ubiquitination		Uniprot
K1068	Ubiquitination		Uniprot
K1075	Ubiquitination		Uniprot
K1080	Ubiquitination		Uniprot
K1088	Ubiquitination		Uniprot
S1121	Phosphorylation		Uniprot
K1129	Ubiquitination		Uniprot
K1136	Ubiquitination		Uniprot
K1163	Ubiquitination		Uniprot
S1202	Phosphorylation		Uniprot
K1231	Ubiquitination		Uniprot
T1247	Phosphorylation		Uniprot
S1248	Phosphorylation		Uniprot
S1255	Phosphorylation		Uniprot
S1259	Phosphorylation		Uniprot
S1274	Phosphorylation		Uniprot
S1282	Phosphorylation		Uniprot
K1299	Ubiquitination		Uniprot
K1305	Ubiquitination		Uniprot
K1314	Ubiquitination		Uniprot
Y1315	Phosphorylation		Uniprot
T1319	Phosphorylation		Uniprot
S1324	Phosphorylation		Uniprot
S1326	Phosphorylation		Uniprot
K1334	Ubiquitination		Uniprot
K1341	Acetylation		Uniprot
K1341	Ubiquitination		Uniprot
S1353	Phosphorylation		Uniprot
S1387	Phosphorylation		Uniprot
K1410	Ubiquitination		Uniprot
S1416	Phosphorylation		Uniprot
K1419	Ubiquitination		Uniprot
K1431	Ubiquitination		Uniprot
S1486	Phosphorylation		Uniprot
S1494	Phosphorylation		Uniprot
S1510	Phosphorylation		Uniprot
K1539	Ubiquitination		Uniprot
T1541	Phosphorylation		Uniprot
S1543	Phosphorylation		Uniprot
K1547	Ubiquitination		Uniprot
S1554	Phosphorylation		Uniprot
K1565	Ubiquitination		Uniprot
K1577	Acetylation		Uniprot
K1579	Ubiquitination		Uniprot
K1600	Ubiquitination		Uniprot
S1651	Phosphorylation		Uniprot
S1652	Phosphorylation		Uniprot
S1654	Phosphorylation		Uniprot
T1660	Phosphorylation		Uniprot
S1670	Phosphorylation		Uniprot
S1674	Phosphorylation		Uniprot
T1678	Phosphorylation		Uniprot
T1685	Phosphorylation		Uniprot
K1687	Ubiquitination		Uniprot
S1722	Phosphorylation		Uniprot
S1726	Phosphorylation		Uniprot
T1729	Phosphorylation		Uniprot
Y1731	Phosphorylation		Uniprot
S1747	Phosphorylation		Uniprot
S1750	Phosphorylation		Uniprot
K1777	Ubiquitination		Uniprot
K1810	Ubiquitination		Uniprot
K1830	Ubiquitination		Uniprot
S1840	Phosphorylation		Uniprot
S1843	Phosphorylation		Uniprot
K1845	Ubiquitination		Uniprot
Y1848	Phosphorylation		Uniprot
S1850	Phosphorylation		Uniprot
T1862	Phosphorylation		Uniprot
S1863	Phosphorylation		Uniprot
S1868	Phosphorylation		Uniprot
K1888	Ubiquitination		Uniprot
S1892	Phosphorylation		Uniprot
S1913	Phosphorylation		Uniprot
Y1920	Phosphorylation		Uniprot
K1973	Ubiquitination		Uniprot
T1974	Phosphorylation		Uniprot
T1975	Phosphorylation		Uniprot
S1981	Phosphorylation		Uniprot
K1983	Ubiquitination		Uniprot
K1987	Ubiquitination		Uniprot
K2012	Ubiquitination		Uniprot
K2028	Ubiquitination		Uniprot
T2031	Phosphorylation		Uniprot
K2036	Ubiquitination		Uniprot
K2042	Ubiquitination		Uniprot
S2048	Phosphorylation		Uniprot
K2051	Ubiquitination		Uniprot
K2063	Ubiquitination		Uniprot
S2080	Phosphorylation		Uniprot
S2082	Phosphorylation		Uniprot
K2086	Ubiquitination		Uniprot
S2090	Phosphorylation		Uniprot
S2108	Phosphorylation		Uniprot
S2180	Phosphorylation		Uniprot
T2189	Phosphorylation		Uniprot
T2207	Phosphorylation		Uniprot
T2216	Phosphorylation		Uniprot
S2227	Phosphorylation		Uniprot
K2305	Ubiquitination		Uniprot
K2325	Ubiquitination		Uniprot
T2377	Phosphorylation		Uniprot
K2394	Ubiquitination		Uniprot
S2416	Phosphorylation		Uniprot
S2417	Phosphorylation		Uniprot
K2425	Ubiquitination		Uniprot
K2467	Ubiquitination		Uniprot
K2482	Ubiquitination		Uniprot
K2528	Ubiquitination		Uniprot
K2540	Ubiquitination		Uniprot
S2542	Phosphorylation		Uniprot
K2552	Ubiquitination		Uniprot
K2575	Ubiquitination		Uniprot
K2591	Ubiquitination		Uniprot
K2604	Acetylation		Uniprot
S2606	Phosphorylation		Uniprot
T2615	Phosphorylation		Uniprot
K2617	Ubiquitination		Uniprot
K2643	Ubiquitination		Uniprot
K2661	Acetylation		Uniprot
K2665	Ubiquitination		Uniprot
K2675	Ubiquitination		Uniprot
K2709	Ubiquitination		Uniprot
K2731	Ubiquitination		Uniprot
K2735	Ubiquitination		Uniprot
K2743	Ubiquitination		Uniprot
T2764	Phosphorylation		Uniprot
K2779	Acetylation		Uniprot
Y2780	Phosphorylation		Uniprot
K2809	Ubiquitination		Uniprot
T2840	Phosphorylation		Uniprot
K2844	Ubiquitination		Uniprot
K2863	Ubiquitination		Uniprot
S2897	Phosphorylation		Uniprot
S2900	Phosphorylation		Uniprot
S2911	Phosphorylation		Uniprot
S2915	Phosphorylation		Uniprot
S2922	Phosphorylation		Uniprot
S2923	Phosphorylation		Uniprot
K2927	Ubiquitination		Uniprot
S2935	Phosphorylation		Uniprot
S2936	Phosphorylation		Uniprot
T2946	Phosphorylation		Uniprot
T2949	Phosphorylation		Uniprot
S2952	Phosphorylation		Uniprot
S2954	Phosphorylation		Uniprot
K2987	Ubiquitination		Uniprot
T2994	Phosphorylation		Uniprot
K2996	Ubiquitination		Uniprot
T2997	Phosphorylation		Uniprot
S2998	Phosphorylation		Uniprot
Y3000	Phosphorylation		Uniprot
T3005	Phosphorylation		Uniprot
S3012	Phosphorylation		Uniprot
K3019	Ubiquitination		Uniprot
S3023	Phosphorylation		Uniprot
S3026	Phosphorylation		Uniprot
S3035	Phosphorylation		Uniprot
S3036	Phosphorylation		Uniprot
S3043	Phosphorylation		Uniprot
S3054	Phosphorylation		Uniprot
S3058	Phosphorylation		Uniprot
T3060	Phosphorylation		Uniprot
S3079	Phosphorylation		Uniprot
T3081	Phosphorylation		Uniprot
S3083	Phosphorylation		Uniprot

Research Backgrounds

P49454_CENPF_HUMAN

Function:

PTMs:

Hyperphosphorylated during mitosis.

Subcellular Location:

Cytoplasm>Perinuclear region. Nucleus matrix. Chromosome>Centromere>Kinetochore. Cytoplasm>Cytoskeleton>Spindle.
Note: Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.

Subunit Structure:

Interacts with and STX4 (via C-terminus) (By similarity). Interacts (via N-terminus) with RBL1, RBL2 and SNAP25 (By similarity). Self-associates. Interacts with CENP-E and BUBR1 (via C-terminus). Interacts (via C-terminus) with NDE1, NDEL1 and RB1.

Family&Domains:

Belongs to the centromere protein F family.

References

1). The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer. International Journal of Biological Sciences, 2020 (PubMed: 32210727) [IF=8.2]

Application: WB Species: human Sample: MCF-7, T47D, MDA-MB-231 and MDA-MB-231/ADR cells

Figure 5.| CENPF-mediated transcriptional regulation of CHK1 by ADR.K After transfection with CENPF-siRNA-1 or CENPF-siRNA-2, CHK1 protein was analyzed by western blot. In MCF-7, T47D, MDA-MB-231 and MDA-MB-231/ADR cells, CENPF inhibition caused significant downregulation of CHK1. Additionally, with MDA-MB-231 exposed to ADR, silencing CENPF also led to a reduction in CHK1 protein. Data shown represent the means (± SD) of three independent experiments; *P < 0.05, **P < 0.01, ***P < 0.001; NS, not significant

2). CENPF knockdown inhibits adriamycin chemoresistance in triple-negative breast cancer via the Rb-E2F1 axis. Scientific Reports, 2023 (PubMed: 36720923) [IF=3.8]

Application: WB Species: Human Sample: MDA-MB-231 and MDA-MB-231/ADR cells

Figure 3 CENPF regulates ADR-induced G2/M phase arrest through Chk1. (A) Flow cytometry was performed to determine the effects of CENPF inhibition on cell cycle distribution with or without adriamycin in MDA-MB-231 cells. (B,C) The effect of CENPF inhibition on cell proliferation in MDA-MB-231 (B) or MDA-MB-231/ADR (C) cells. (D) Cell proliferation was determined by EdU assay. (E,F) Using RT‒qPCR, the mRNA level of CHK1 was detected in MDA-MB-231 (E) or MDA-MB-231/ADR (F) cells with CENPF knockdown. (G) Western blot analysis of the effect of CENPF knockdown on Chk1 expression and phosphorylation in MDA-MB-231 and MDA-MB-231/ADR cells exposed to adriamycin. Data shown represent the means (± SD) of three independent experiments; **P

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CENPF Antibody - #DF2310