CENPN Antibody - #DF2315

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Product: CENPN Antibody
Catalog: DF2315
Description: Rabbit polyclonal antibody to CENPN
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 40 kDa; 40kD(Calculated).
Uniprot: Q96H22
RRID: AB_2839539

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
CENPN Antibody detects endogenous levels of total CENPN.
RRID:
AB_2839539
Cite Format: Affinity Biosciences Cat# DF2315, RRID:AB_2839539.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BM 039; BM 309; BM-309; BM039; C16orf60; CENP N; CENP-N; Cenpn; CENPN_HUMAN; Centromere protein N; FLJ13607; FLJ22660; ICEN 32; ICEN32; Interphase centromere complex protein 32;

Immunogens

Immunogen:
Uniprot:

Q96H22(CENPN_HUMAN) >>Visit HPA database.

Gene(ID):
CENPN(55839)
Description:
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
Sequence:
MDETVAEFIKRTILKIPMNELTTILKAWDFLSENQLQTVNFRQRKESVVQHLIHLCEEKRASISDAALLDIIYMQFHQHQKVWEVFQMSKGPGEDVDLFDMKQFKNSFKKILQRALKNVTVSFRETEENAVWIRIAWGTQYTKPNQYKPTYVVYYSQTPYAFTSSSMLRRNTPLLGQALTIASKHHQIVKMDLRSRYLDSLKAIVFKQYNQTFETHNSTTPLQERSLGLDINMDSRIIHENIVEKERVQRITQETFGDYPQPQLEFAQYKLETKFKSGLNGSILAEREEPLRCLIKFSSPHLLEALKSLAPAGIADAPLSPLLTCIPNKRMNYFKIRDK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
92
Chicken
92
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96H22 As Substrate

Site PTM Type Enzyme
K10 Ubiquitination
K15 Ubiquitination
K45 Ubiquitination
K90 Ubiquitination
K102 Ubiquitination
K117 Ubiquitination
T172 Phosphorylation
T180 Phosphorylation
S183 Phosphorylation
K190 Ubiquitination
K202 Ubiquitination
K207 Ubiquitination
S226 Phosphorylation
S235 Phosphorylation
K245 Ubiquitination
K270 Ubiquitination
K276 Ubiquitination
S277 Phosphorylation
S282 Phosphorylation
K296 Ubiquitination
S299 Phosphorylation
S320 Phosphorylation
K329 Ubiquitination
Y333 Phosphorylation

Research Backgrounds

Function:

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPN is the first protein to bind specifically to CENPA nucleosomes and the direct binding of CENPA nucleosomes by CENPN is required for centromere assembly. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.

Subcellular Location:

Nucleus. Chromosome>Centromere>Kinetochore.
Note: Localizes exclusively in the kinetochore domain of centromeres. Kinetochore-bound levels decrease when cells enter mitosis and increase again when cells exit mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts directly with CENPA. Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1.

Family&Domains:

Belongs to the CENP-N/CHL4 family.

References

1). Dynamic phosphorylation of CENP-N by CDK1 guides accurate chromosome segregation in mitosis. Journal of Molecular Cell Biology, 2022 (PubMed: 37365681) [IF=5.3]

Application: WB    Species: Human    Sample:

Figure 1 The interaction between CENP-L and CENP-N is required for accurate mitotic progression. (A) The binding interface between CENP-L and CENP-N. A close-up view of the key β-strand of CENP-N at the CENP-L–CENP-N interface is shown in magenta. (B) Immunoprecipitation analyses show that the TAT-CENP-N271–300 peptide disrupts the interaction between CENP-L and CENP-N. (C) Disrupting the CENP-L–CENP-N interaction causes chromosome misalignment. Representative images illustrating mitotic progression in mCherry-H2B-expressing cells treated with scramble control peptide or 5 μM TAT-CENP-N271–300 peptide in Opti-MEM for 60 min. Live cell imaging analyses were performed under a DeltaVision microscope. Images were acquired at the indicated time points after the start of nuclear envelope breakdown (control: n = 25, TAT-CENP-N271–300: n = 34). Scale bar, 10 μm. (D) Bar graphs illustrating the percentages of various phenotypes of cells treated as in C.
2). CENPN Acts as a Novel Biomarker that Correlates With the Malignant Phenotypes of Glioma Cells. Frontiers in Genetics, 2021 (PubMed: 34646306) [IF=3.7]

Application: WB    Species: Human    Sample:

FIGURE 6 Effects of CENPN knockdown on glioma cells in vitro. (A,B) Expression of CENPN detected after transfection with siRNA by PCR and western blot. *p < 0.05, **p < 0.01, ***p < 0.001. (C) CCK-8 assay was used to estimate the effect of CENPN knockdown on cell proliferation. *p < 0.05, **p < 0.01, ***p < 0.001, siCENPN-i vs siScr group; #p < 0.05, ##p < 0.01, ###p < 0.001, siCENPN-ii vs siScr group. (D) Cell proliferation was estimated by colony formation assay. (E) Wound-healing assay was used to analyze the migration ability of LN229 and U251 cells. Scale bar, 400 μm. (F,G) Invasive ability and the degree of apoptosis were estimated by Transwell assay (F) and FITC annexin V (G), respectively. Data are the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA. Scale bar, 200 μm.
3). Centromere protein N promotes lung adenocarcinoma progression by activating PI3K/AKT signaling pathway. Genes & Genomics, 2022 (PubMed: 35150399) [IF=1.6]

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