CXCR6 Antibody | Affinity Biosciences

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CXCR6 Antibody - Figure 5 PJI patients with high CXCR6 showed a higher recurrence rate.

Product:	CXCR6 Antibody
Catalog:	DF2328
Description:	Rabbit polyclonal antibody to CXCR6
Application:	WB IHC
Cited expt.:	IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Rabbit, Dog
Mol.Wt.:	44 kDa; 39kD(Calculated).
Uniprot:	O00574
RRID:	AB_2839552

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:100
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Rabbit(92%), Dog(83%)

Clonality:

Polyclonal

Specificity:

CXCR6 Antibody detects endogenous levels of total CXCR6.

RRID:

AB_2839552
Cite Format: Affinity Biosciences Cat# DF2328, RRID:AB_2839552.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C X C chemokine receptor type 6; C-X-C chemokine receptor type 6; CD 186; CD186; CD186 antigen; CDw186; Chemokine (C X C motif) receptor 6; Chemokine C X C motif receptor 6; Chemokine CXC motif receptor 6; Chemokine receptor 6; CXC chemokine receptor type 6; CXC R6; CXC-R6; CXCR 6; CXCR-6; CXCR6; CXCR6_HUMAN; G protein coupled receptor; G protein coupled receptor bonzo; G protein coupled receptor STRL 33; G protein coupled receptor STRL33; G protein coupled receptor TYMSTR; G-protein coupled receptor bonzo; G-protein coupled receptor STRL33; STRL 33; STRL33; TYMSTR;

Immunogens

Immunogen:

A synthesized peptide derived from human CXCR6, corresponding to a region within C-terminal amino acids.

Uniprot:

O00574(CXCR6_HUMAN) >>Visit HPA database.

Gene(ID):

CXCR6(10663)

Expression:

O00574 CXCR6_HUMAN:

Expressed in lymphoid tissues and activated T cells.

Description:

Receptor for the C-X-C chemokine CXCL16. Used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1.

Sequence:

O00574 CXCR6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAEHDYHEDYGFSSFNDSSQEEHQDFLQFSKVFLPCMYLVVFVCGLVGNSLVLVISIFYHKLQSLTDVFLVNLPLADLVFVCTLPFWAYAGIHEWVFGQVMCKSLLGIYTINFYTSMLILTCITVDRFIVVVKATKAYNQQAKRMTWGKVTSLLIWVISLLVSLPQIIYGNVFNLDKLICGYHDEAISTVVLATQMTLGFFLPLLTMIVCYSVIIKTLLHAGGFQKHRSLKIIFLVMAVFLLTQMPFNLMKFIRSTHWEYYAMTSFHYTIMVTEAIAYLRACLNPVLYAFVSLKFRKNFWKLVKDIGCLPYLGVSHQWKSSEDNSKTFSASHNVEATSMFQL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Dog

Pig

Horse

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

O00574_CXCR6_HUMAN

Function:

Receptor for the C-X-C chemokine CXCL16. Used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Expressed in lymphoid tissues and activated T cells.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

References

1). CXCL16/CXCR6/TGF-β Feedback Loop Between M-MDSCs and Treg Inhibits Anti-Bacterial Immunity During Biofilm Infection. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 39716908) [IF=15.1]

Application: IHC Species: Mouse Sample:

Figure 5 PJI patients with high CXCR6 showed a higher recurrence rate. a) The Volcano plot showed differentially expressed genes in PJI versus AF patients. The x and y axes represented the log2 fold change and p-value, respectively, based on the Mann-Whitney U test. Red dots: upregulated genes, blue dots: downregulated genes. b) The heatmap showed the distribution of 28 types of immune cells in PJI and AF. c) The bar chart showed the difference in proportion of M-MDSCs and Treg between PJI and AF (PJI, n = 18; AF, n = 18). Unpaired t test; ***p < 0.001. d) The heatmap showed the characteristic genes of chemokines, PJI markers, cell markers and immune checkpoint between PJI and AF. e–l) The violin plots showed part of the differential genes (CXCL16, CXCR6, S100A8, S100A9, FOXP3, CTLA4, TIGIT and LAG3) between PJI and AF as shown in (d). Unpaired t test; nsp > 0.05, ***p < 0.001, ****p < 0.0001. m,n) Quantitative analysis of immunohistochemical staining for CXCL16 and CXCR6 (PJI, n = 80; AF, n = 25; OA = 25). 40x magnification. Scale bar, 20 mm. Unpaired t test; **p < 0.01, ***p < 0.001. o) The Kaplan-Meier curve showed the recurrence curves of PJI patients characterized by either low or high expression of CXCR6 (CXCR6-high PJI, n = 30; CXCR6-low PJI, n = 30). Significance was calculated using the log-rank test. p,q) Quantitative analysis of Harris Hip score and HSS knee score in CXCR6-high PJI and CXCR6-low PJI group (CXCR6-high PJI, n = 30; CXCR6-low PJI, n = 30). Unpaired t test

2). Targeting CXCL16-expressing macrophages with a biomimetic nanocarrier system attenuates cartilage degeneration in osteoarthritis. Journal of controlled release : official journal of the Controlled Release Society, 2026 (PubMed: 41707770) [IF=10.5]

3). Targeting of Tumoral NAC1 Mitigates Myeloid-Derived Suppressor Cell-Mediated Immunosuppression and Potentiates Anti-PD-1 Therapy in Ovarian Cancer. Cancer immunology research, 2025 (PubMed: 39531476) [IF=8.1]

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CXCR6 Antibody - #DF2328