CYLC2 Antibody - #DF2331
(4)
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Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
CYLC2 Antibody detects endogenous levels of total CYLC2.
RRID:
AB_2839555
Cite Format: Affinity Biosciences Cat# DF2331, RRID:AB_2839555.
Cite Format: Affinity Biosciences Cat# DF2331, RRID:AB_2839555.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
CYL2; CYLC 2; Cylicin 2; Cylicin basic protein of sperm head cytoskeleton 2; Cylicin II; Cylicin2; MGC129591; Multiple band polypeptide II;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q14093 CYLC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLPRFQRVNFGPYDNYIPVSELSKKSWNQQHFALLFPKPQRPGTKRRSKPSQIRDNTVSIIDEEQLRGDRRQPLWMYRSLMRISERPSVYLAARRQPLKPTRTVEVDSKAAEIGKKGEDKTTQKDTTDSESELKQGKKDSKKGKDIEKGKEEKLDAKKDSKKGKKDAEKGKDSATESEDEKGGAKKDNKKDKKDSNKGKDSATESEGEKGGTEKDSKKGKKDSKKGKDSAIELQAVKADEKKDEDGKKDANKGDESKDAKKDAKEIKKGKKDKKKPSSTDSDSKDDVKKESKKDATKDAKKVAKKDTEKESADSKKDAKKNAKKDAKKDAKKNAKKDEKKDAKKKGK
PTMs - Q14093 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R87 | Methylation | Uniprot | |
| R95 | Methylation | Uniprot | |
| R96 | Methylation | Uniprot | |
| T104 | Phosphorylation | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| K139 | Acetylation | Uniprot | |
| K143 | Acetylation | Uniprot | |
| K145 | Acetylation | Uniprot | |
| K172 | Acetylation | Uniprot | |
| K182 | Acetylation | Uniprot | |
| K186 | Acetylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| T204 | Phosphorylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| T213 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| T297 | Phosphorylation | Uniprot | |
| K302 | Acetylation | Uniprot | |
| K305 | Acetylation | Uniprot | |
| K310 | Ubiquitination | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| K329 | Ubiquitination | Uniprot |
Research Backgrounds
Function:
Possible architectural role during spermatogenesis. May be involved in spermatid differentiation.
Subcellular Location:
Cytoplasm>Cytoskeleton>Perinuclear theca>Calyx.
Note: Sperm head cytoskeletal structure.
Tissue Specificity:
Testis.
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For Research Use Only.
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