Product: EEF2 Antibody
Catalog: BF0405
Description: Mouse monoclonal antibody to EEF2
Application: WB IHC IF/ICC ELISA
Reactivity: Human, Mouse
Mol.Wt.: 95kDa; 95kD(Calculated).
Uniprot: P13639
RRID: AB_2833901

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 50ul $250 In stock
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 200ul $450 In stock

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Product Info

ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Monoclonal [AFB1877]
EEF2 antibody detects endogenous levels of total EEF2.
Cite Format: Affinity Biosciences Cat# BF0405, RRID:AB_2833901.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


EEF 2; Eef2; EF-2; EF2; EF2_HUMAN; Elongation factor 2; Eukaryotic translation elongation factor 2; Polypeptidyl tRNA translocase; SCA26;



Purified recombinant fragment of human EEF2 expressed in E. Coli.

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. (provided by RefSeq)

PTMs - P13639 As Substrate

Site PTM Type Enzyme
R10 Methylation
K15 Ubiquitination
S23 Phosphorylation
K32 Ubiquitination
S38 Phosphorylation
C41 S-Nitrosylation
K42 Acetylation
K42 Ubiquitination
S48 Phosphorylation
R50 Methylation
T54 Phosphorylation O00418 (EEF2K)
T57 Phosphorylation O00418 (EEF2K)
T59 Phosphorylation O00418 (EEF2K)
C67 S-Nitrosylation
S72 Phosphorylation
T73 Phosphorylation
S76 Phosphorylation
Y79 Phosphorylation
K90 Ubiquitination
K93 Ubiquitination
K152 Ubiquitination
K159 Ubiquitination
Y175 Phosphorylation
T177 Phosphorylation
S197 Phosphorylation
K235 Acetylation
K235 Ubiquitination
K239 Acetylation
K239 Sumoylation
K239 Ubiquitination
K251 Ubiquitination
K252 Acetylation
K252 Ubiquitination
K258 Acetylation
K259 Ubiquitination
R264 Methylation
Y265 Phosphorylation
K272 Acetylation
K272 Methylation
K272 Ubiquitination
S274 Phosphorylation
K275 Acetylation
K275 Ubiquitination
K283 Ubiquitination
T288 Phosphorylation
C290 S-Nitrosylation
K308 Acetylation
K308 Ubiquitination
K314 Acetylation
K314 Ubiquitination
K318 Acetylation
K318 Ubiquitination
K322 Acetylation
K322 Sumoylation
K322 Ubiquitination
S325 Phosphorylation
K330 Ubiquitination
K333 Ubiquitination
K337 Acetylation
K337 Ubiquitination
T355 Phosphorylation
S360 Phosphorylation
K366 Acetylation
K366 Ubiquitination
C369 S-Nitrosylation
Y373 Phosphorylation
K386 Ubiquitination
K391 Acetylation
K391 Ubiquitination
S399 Phosphorylation
K400 Ubiquitination
T404 Phosphorylation
K407 Acetylation
K407 Methylation
K407 Ubiquitination
R409 Methylation
Y411 Phosphorylation
S418 Phosphorylation
K426 Acetylation
K426 Ubiquitination
R428 Methylation
Y434 Phosphorylation
T435 Phosphorylation P24941 (CDK2)
K438 Acetylation
K438 Ubiquitination
K439 Acetylation
K439 Ubiquitination
Y443 Phosphorylation
K445 Acetylation
K445 Methylation
K445 Ubiquitination
T450 Phosphorylation
K481 Ubiquitination
R495 Methylation
K498 Acetylation
K498 Methylation
K498 Ubiquitination
S500 Phosphorylation
S502 Phosphorylation
K512 Ubiquitination
K519 Methylation
K519 Ubiquitination
K525 Methylation
K525 Ubiquitination
R526 Methylation
K529 Sumoylation
S530 Phosphorylation
S541 Phosphorylation
C567 S-Nitrosylation
K571 Ubiquitination
K572 Ubiquitination
Y579 Phosphorylation
T582 Phosphorylation
S584 Phosphorylation
S587 Phosphorylation
C591 S-Nitrosylation
S593 Phosphorylation
K594 Methylation
K594 Ubiquitination
S595 Phosphorylation P24941 (CDK2)
K598 Ubiquitination
K605 Ubiquitination
K619 Ubiquitination
S623 Phosphorylation
K629 Ubiquitination
K638 Acetylation
K638 Methylation
K638 Ubiquitination
K648 Acetylation
K648 Ubiquitination
C651 S-Nitrosylation
K667 Ubiquitination
Y671 Phosphorylation
K676 Ubiquitination
S678 Phosphorylation
K688 Acetylation
K688 Ubiquitination
R698 Methylation
R716 Methylation
T724 Phosphorylation
R726 Methylation
C728 S-Nitrosylation
Y730 Phosphorylation
S732 Phosphorylation
R739 Methylation
Y745 Phosphorylation
Y760 Phosphorylation
K766 Ubiquitination
R767 Methylation
T779 Phosphorylation
S793 Phosphorylation
S802 Phosphorylation
K842 Ubiquitination
K845 Ubiquitination
K857 Acetylation
K857 Ubiquitination

Research Backgrounds


Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.


Phosphorylation by EF-2 kinase completely inactivates EF-2; it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment.

Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine (By similarity).

(Microbial infection) Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.


Proteolytically processed at two sites following phosphorylation by CSK.

SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9. Interacts with RBPMS2.


Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

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