TAB3 Antibody - #DF2369

(2)  
Product: TAB3 Antibody
Catalog: DF2369
Description: Rabbit polyclonal antibody to TAB3
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 79 kDa; 79kD(Calculated).
Uniprot: Q8N5C8
RRID: AB_2839577

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
TAB3 Antibody detects endogenous levels of total TAB3.
RRID:
AB_2839577
Cite Format: Affinity Biosciences Cat# DF2369, RRID:AB_2839577.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MAP3K7IP 3; Mitogen-activated protein kinase kinase kinase 7-interacting protein 3; NAP1; NF-kappa-B-activating protein 1; NFkB activating protein 1; TAB-3; Tab3; TAB3_HUMAN; TAK1 binding protein 3; TAK1-binding protein 3; TGF-beta-activated kinase 1 and MAP3K7-binding protein 3; TGF-beta-activated kinase 1-binding protein 3;

Immunogens

Immunogen:
Uniprot:

Q8N5C8(TAB3_HUMAN) >>Visit HPA database.

Gene(ID):
TAB3(257397)
Expression:
Q8N5C8 TAB3_HUMAN:

Widely expressed. Constitutively overexpressed in certain tumor tissues. Isoform 1 is a major transcript while isoform 2 is a minor transcript.

Description:
Adapter linking MAP3K7/TAK1 and TRAF6 or TRAF2. Mediator of MAP3K7 activation, respectively in the IL1 and TNF signaling pathways. Plays a role in activation of NF-kappa-B and AP1 transcription factor. Isoform 2 may be an oncogenic factor.
Sequence:
MAQSSPQLDIQVLHDLRQRFPEIPEGVVSQCMLQNNNNLEACCRALSQESSKYLYMEYHSPDDNRMNRNRLLHINLGIHSPSSYHPGDGAQLNGGRTLVHSSSDGHIDPQHAAGKQLICLVQEPHSAPAVVAATPNYNPFFMNEQNRSAATPPSQPPQQPSSMQTGMNPSAMQGPSPPPPPPSYMHIPRYSTNPITVTVSQNLPSGQTVPRALQILPQIPSNLYGSPGSIYIRQTSQSSSGRQTPQSTPWQSSPQGPVPHYSQRPLPVYPHQQNYQPSQYSPKQQQIPQSAYHSPPPSQCPSPFSSPQHQVQPSQLGHIFMPPSPSTTPPHPYQQGPPSYQKQGSHSVAYLPYTASSLSKGSMKKIEITVEPSQRPGTAINRSPSPISNQPSPRNQHSLYTATTPPSSSPSRGISSQPKPPFSVNPVYITYTQPTGPSCTPSPSPRVIPNPTTVFKITVGRATTENLLNLVDQEERSAAPEPIQPISVIPGSGGEKGSHKYQRSSSSGSDDYAYTQALLLHQRARMERLAKQLKLEKEELERLKSEVNGMEHDLMQRRLRRVSCTTAIPTPEEMTRLRSMNRQLQINVDCTLKEVDLLQSRGNFDPKAMNNFYDNIEPGPVVPPKPSKKDSSDPCTIERKARRISVTSKVQADIHDTQAAAADEHRTGSTQSPRTQPRDEDYEGAPWNCDSCTFLNHPALNRCEQCEMPRYT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Chicken
100
Rabbit
100
Sheep
92
Xenopus
73
Bovine
50
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N5C8 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S5 Phosphorylation
Y53 Phosphorylation
Y55 Phosphorylation
Y58 Phosphorylation
S60 Phosphorylation
S80 Phosphorylation
S82 Phosphorylation
S83 Phosphorylation
Y84 Phosphorylation
T97 Phosphorylation
S101 Phosphorylation
S102 Phosphorylation
S103 Phosphorylation
S126 Phosphorylation
T134 Phosphorylation
Y224 Phosphorylation
R264 Methylation
K283 Methylation
T354 Phosphorylation
S385 Phosphorylation
T404 Phosphorylation
S407 Phosphorylation
S408 O-Glycosylation
S408 Phosphorylation
S409 Phosphorylation
Y428 Phosphorylation
T440 Phosphorylation
S442 Phosphorylation
S444 Phosphorylation
S492 Phosphorylation
S504 Phosphorylation
S506 Phosphorylation
Y512 Phosphorylation
S563 Phosphorylation
Y613 Phosphorylation
S627 Phosphorylation
S645 Phosphorylation
T657 Phosphorylation

Research Backgrounds

Function:

Adapter linking MAP3K7/TAK1 and TRAF6 or TRAF2. Mediator of MAP3K7 activation, respectively in the IL1 and TNF signaling pathways. Plays a role in activation of NF-kappa-B and AP1 transcription factor. Isoform 2 may be an oncogenic factor.

PTMs:

Ubiquitinated; following IL1 stimulation or TRAF6 overexpression.

Phosphorylated at Ser-506 by MAPKAPK2 and MAPKAPK3 following IL1 treatment.

Tissue Specificity:

Widely expressed. Constitutively overexpressed in certain tumor tissues. Isoform 1 is a major transcript while isoform 2 is a minor transcript.

Subunit Structure:

Interacts with TAB1, TAB2, MAP3K7, TRAF2 and TRAF6. The minimal TAB3-containing complex (TAB1-MAP3K7-TAB3) appears not to contain TAB2. However, it seems sensible to consider that TAB2 may also join this complex and may act in a cooperative manner with TAB3. Interacts with WDR34 (via the WD domains). Interacts with RBCK1. Binds 'Lys-63'-linked polyubiquitin chains. Interacts with TRIM5.

Family&Domains:

The RanBP2-type zinc finger (NZF) mediates binding to two consecutive 'Lys-63'-linked ubiquitins.

Research Fields

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

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