PHLDA3 Antibody - #DF2389

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Product: PHLDA3 Antibody
Catalog: DF2389
Description: Rabbit polyclonal antibody to PHLDA3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Rabbit
Mol.Wt.: 14 kDa; 14kD(Calculated).
Uniprot: Q9Y5J5
RRID: AB_2839597

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
PHLDA3 Antibody detects endogenous levels of total PHLDA3.
RRID:
AB_2839597
Cite Format: Affinity Biosciences Cat# DF2389, RRID:AB_2839597.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

PHLA3_HUMAN; PHLDA 3; phlda3; Pleckstrin homology like domain family A member 2; Pleckstrin homology like domain family A member 3; Pleckstrin homology-like domain family A member 3; TDAG51/Ipl homolog 1; TDAG51/Ipl homologue 1; TIH 1; TIH1;

Immunogens

Immunogen:
Uniprot:

Q9Y5J5(PHLA3_HUMAN) >>Visit HPA database.

Gene(ID):
PHLDA3(23612)
Expression:
Q9Y5J5 PHLA3_HUMAN:

Widely expressed with lowest expression in liver and spleen.

Description:
p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor.
Sequence:
MTAAATATVLKEGVLEKRSGGLLQLWKRKRCVLTERGLQLFEAKGTGGRPKELSFARIKAVECVESTGRHIYFTLVTEGGGEIDFRCPLEDPGWNAQITLGLVKFKNQQAIQTVRARQSLGTGTLVS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
90
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y5J5 As Substrate

Site PTM Type Enzyme
K17 Ubiquitination
K27 Ubiquitination
K44 Ubiquitination
K51 Ubiquitination
K106 Methylation
K106 Ubiquitination
S119 Phosphorylation
T122 Phosphorylation

Research Backgrounds

Function:

p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its direct transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May act as a tumor suppressor.

Subcellular Location:

Cytoplasm. Membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with lowest expression in liver and spleen.

Family&Domains:

The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.

Belongs to the PHLDA3 family.

References

1). PHLDA3 exerts an antitumor function in prostate cancer by down-regulating Wnt/β-catenin pathway via inhibition of Akt. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2021 (PubMed: 34303965) [IF=2.5]

Application: WB    Species: Human    Sample: prostate cancer cells

Fig. 1. The expression and prognostic value of PHLDA3 in prostate cancer. The expression profile of PHLDA3 in prostate cancer was assessed via TCGA data through the online databases (A) GEPIA2 (*p < 0.05) and (B) UALCAN (***p < 0.001). (C) Levels of PHLDA3 mRNA in prostate cancer specimens were measured via RT-qPCR (***p < 0.001). (D). Protein levels of PHLDA3 in prostate cancer specimens were determined via western blotting (***p < 0.001). (E) Kaplan-Meier analysis of overall survival in prostate cancer patients with different levels of PHLDA3, obtained from the UALCAN database. (F) Expression levels of PHLDA3 in prostate cancer cell lines (DU-145, PC-3, LNCaP and 22Rv1) were examined via western blot (*p < 0.05 and **p < 0.01 vs. RWPE-1). (G) The promoter methylation level of PHLDA3 in prostate cancer was analyzed via TCGA data through the UALCAN database (****p < 0.0001). The demethylation compound 5-AZA-CdR was added to cells at a concentration of 10 m M, followed by incubation for 72 h. Cells treated with dimethylsulfoxide (DMSO) were used as a control. (H) The effects of 5-AZA-CdR treatment on the expression of PHLDA3 were assessed via western blot (**p < 0.01).

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