IFRD1 Antibody - #DF2392

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Product: IFRD1 Antibody
Catalog: DF2392
Description: Rabbit polyclonal antibody to IFRD1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 50 kDa; 50kD(Calculated).
Uniprot: O00458
RRID: AB_2839600

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
IFRD1 Antibody detects endogenous levels of total IFRD1.
RRID:
AB_2839600
Cite Format: Affinity Biosciences Cat# DF2392, RRID:AB_2839600.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

12 O tetradecanoylphorbol 13 acetate induced sequence 7; IFRD1; IFRD1_HUMAN; interferon related developmental regulator 1; Interferon-related developmental regulator 1; Nerve growth factor inducible protein PC4; Nerve growth factor-inducible protein PC4; PC4; Pheochromocytoma cell 4; TIS7; TPA induced sequence 7;

Immunogens

Immunogen:
Uniprot:

O00458(IFRD1_HUMAN) >>Visit HPA database.

Gene(ID):
IFRD1(3475)
Expression:
O00458 IFRD1_HUMAN:

Expressed in a variety of tissues.

Description:
Could play a role in regulating gene activity in the proliferative and/or differentiative pathways induced by NGF. May be an autocrine factor that attenuates or amplifies the initial ligand-induced signal.
Sequence:
MPKNKKRNTPHRGSSAGGGGSGAAAATAATAGGQHRNVQPFSDEDASIETMSHCSGYSDPSSFAEDGPEVLDEEGTQEDLEYKLKGLIDLTLDKSAKTRQAALEGIKNALASKMLYEFILERRMTLTDSIERCLKKGKSDEQRAAAALASVLCIQLGPGIESEEILKTLGPILKKIICDGSASMQARQTCATCFGVCCFIATDDITELYSTLECLENIFTKSYLKEKDTTVICSTPNTVLHISSLLAWTLLLTICPINEVKKKLEMHFHKLPSLLSCDDVNMRIAAGESLALLFELARGIESDFFYEDMESLTQMLRALATDGNKHRAKVDKRKQRSVFRDVLRAVEERDFPTETIKFGPERMYIDCWVKKHTYDTFKEVLGSGMQYHLQSNEFLRNVFELGPPVMLDAATLKTMKISRFERHLYNSAAFKARTKARSKCRDKRADVGEFF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
82
Xenopus
64
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00458 As Substrate

Site PTM Type Enzyme
T9 Phosphorylation
S14 Phosphorylation
S15 Phosphorylation
S21 Phosphorylation
K94 Ubiquitination
K107 Ubiquitination
Y116 Phosphorylation
S162 Phosphorylation
K174 Ubiquitination
K175 Ubiquitination
K325 Ubiquitination
K357 Ubiquitination
K371 Ubiquitination
S427 Phosphorylation
K431 Ubiquitination

Research Backgrounds

Function:

Could play a role in regulating gene activity in the proliferative and/or differentiative pathways induced by NGF. May be an autocrine factor that attenuates or amplifies the initial ligand-induced signal (By similarity).

Tissue Specificity:

Expressed in a variety of tissues.

Subunit Structure:

Interacts with PSIP1/LEDGF.

Family&Domains:

Belongs to the IFRD family.

References

1). Valproic Acid Stimulates Hippocampal Neurogenesis via Activating the Wnt/β-Catenin Signaling Pathway in the APP/PS1/Nestin-GFP Triple Transgenic Mouse Model of Alzheimer's Disease. Frontiers in Aging Neuroscience, 2019 (PubMed: 30971911) [IF=4.1]

Application: WB    Species: Mouse    Sample: Hippocampus tissue

Figure 6 VPA inhibits glycogen synthase kinase-3β (GSK-3β) activity and stimulates the Wnt/β-catenin signaling pathway. (A) Western blot analysis of total GSK-3β and p-GSK-3βS9 (pSer9). GAPDH was used as an internal control. (B) Densitometric analysis of total GSK-3β and p-GSK-3βS9 (pSer9). VPA increased pSer9-GSK-3β levels but not total GSK-3β levels in AD mice. (C) Immunoblots of β-catenin, Wnt3a and NeuroD1; β-actin was used as an internal control. (D) Densitometric analysis of β-catenin, Wnt3a and NeuroD1 normalized to β-actin. VPA increased β-catenin, Wnt3a and NeuroD1 levels in AD mice. *P < 0.05, **P < 0.001, ***P < 0.0001.
2). Loureirin B Alleviates Myocardial Ischemia/Reperfusion Injury via Inhibiting PAI-1/TGF-β1/Smad Signaling Pathway. Evidence-based Complementary and Alternative Medicine, 2022 (PubMed: 35535157)

Application: WB    Species: Mice    Sample:

Figure 4 LrB suppressed the expression of PAI-1 and the activation of TGF-β1/Smad signaling pathway in MI/R mice. (a) Representative images showing the distribution of PAI-1 in cardiac tissues after immunofluorescence staining. Scar bar = 50 μm. (b, c) Relative mRNA and protein expression of urokinase plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) in the cardiac tissues. (d) Western blot analysis for TGF-β1R, TGF-β1, p-Smad2/3, and Smad2/3. Relative protein expression of (e) PAI-1, (f) TGF-β1R, (g) TGF-β1, (h) p-Smad2/3, and (i) Smad2/3 in the cardiac tissues. Values are expressed as mean ± SD (n = 6). +++p < 0.001vs. sham group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001vs. MI/R group.

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