SNAPC4 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

SNAPC4 Antibody - Western blot analysis of expression in HEK293 cells ,The lane on the left was treated with the antigen-specific peptide.

SNAPC4 Antibody - DF2405 at 1/100 staining Rat heart tissue by IHC-P.

SNAPC4 Antibody - DF2405 staining HepG2 cells by IF/ICC.

Product:	SNAPC4 Antibody
Catalog:	DF2405
Description:	Rabbit polyclonal antibody to SNAPC4
Application:	WB IHC IF/ICC
Reactivity:	Human, Rat
Prediction:	Bovine, Horse, Sheep, Dog
Mol.Wt.:	160 KD; 159kD(Calculated).
Uniprot:	Q5SXM2
RRID:	AB_2839612

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Bovine(82%), Horse(82%), Sheep(82%), Dog(82%)

Clonality:

Polyclonal

Specificity:

SNAPC4 Antibody detects endogenous levels of total SNAPC4.

RRID:

AB_2839612
Cite Format: Affinity Biosciences Cat# DF2405, RRID:AB_2839612.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ13451; OTTHUMP00000022583; Proximal sequence element binding transcription factor subunit alpha; Proximal sequence element-binding transcription factor subunit alpha; PSE binding factor subunit alpha; PSE-binding factor subunit alpha; PTF subunit alpha; PTFalpha; Small nuclear RNA activating complex polypeptide 4 190kD; Small nuclear RNA activating complex polypeptide 4 190kDa; Small nuclear RNA activating complex polypeptide 4; SNAP190; SNAPc 190 kDa subunit; SNAPc subunit 4; Snapc4; SNPC4_HUMAN; snRNA activating protein complex 190 kDa subunit; snRNA activating protein complex subunit 4; snRNA-activating protein complex 190 kDa subunit; snRNA-activating protein complex subunit 4;

Immunogens

Immunogen:

Uniprot:

Q5SXM2(SNPC4_HUMAN) >>Visit HPA database.

Gene(ID):

SNAPC4(6621)

Sequence:

Q5SXM2 SNPC4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDVDAEREKITQEIKELERILDPGSSGSHVEISESSLESDSEADSLPSEDLDPADPPISEEERWGEASNDEDDPKDKTLPEDPETCLQLNMVYQEVIQEKLAEANLLLAQNREQQEELMRDLAGSKGTKVKDGKSLPPSTYMGHFMKPYFKDKVTGVGPPANEDTREKAAQGIKAFEELLVTKWKNWEKALLRKSVVSDRLQRLLQPKLLKLEYLHQKQSKVSSELERQALEKQGREAEKEIQDINQLPEEALLGNRLDSHDWEKISNINFEGSRSAEEIRKFWQNSEHPSINKQEWSREEEERLQAIAAAHGHLEWQKIAEELGTSRSAFQCLQKFQQHNKALKRKEWTEEEDRMLTQLVQEMRVGSHIPYRRIVYYMEGRDSMQLIYRWTKSLDPGLKKGYWAPEEDAKLLQAVAKYGEQDWFKIREEVPGRSDAQCRDRYLRRLHFSLKKGRWNLKEEEQLIELIEKYGVGHWAKIASELPHRSGSQCLSKWKIMMGKKQGLRRRRRRARHSVRWSSTSSSGSSSGSSGGSSSSSSSSSEEDEPEQAQAGEGDRALLSPQYMVPDMDLWVPARQSTSQPWRGGAGAWLGGPAASLSPPKGSSASQGGSKEASTTAAAPGEETSPVQVPARAHGPVPRSAQASHSADTRPAGAEKQALEGGRRLLTVPVETVLRVLRANTAARSCTQKEQLRQPPLPTSSPGVSSGDSVARSHVQWLRHRATQSGQRRWRHALHRRLLNRRLLLAVTPWVGDVVVPCTQASQRPAVVQTQADGLREQLQQARLASTPVFTLFTQLFHIDTAGCLEVVRERKALPPRLPQAGARDPPVHLLQASSSAQSTPGHLFPNVPAQEASKSASHKGSRRLASSRVERTLPQASLLASTGPRPKPKTVSELLQEKRLQEARAREATRGPVVLPSQLLVSSSVILQPPLPHTPHGRPAPGPTVLNVPLSGPGAPAAAKPGTSGSWQEAGTSAKDKRLSTMQALPLAPVFSEAEGTAPAASQAPALGPGQISVSCPESGLGQSQAPAASRKQGLPEAPPFLPAAPSPTPLPVQPLSLTHIGGPHVATSVPLPVTWVLTAQGLLPVPVPAVVSLPRPAGTPGPAGLLATLLPPLTETRAAQGPRAPALSSSWQPPANMNREPEPSCRTDTPAPPTHALSQSPAEADGSVAFVPGEAQVAREIPEPRTSSHADPPEAEPPWSGRLPAFGGVIPATEPRGTPGSPSGTQEPRGPLGLEKLPLRQPGPEKGALDLEKPPLPQPGPEKGALDLGLLSQEGEAATQQWLGGQRGVRVPLLGSRLPYQPPALCSLRALSGLLLHKKALEHKATSLVVGGEAERPAGALQASLGLVRGQLQDNPAYLLLRARFLAAFTLPALLATLAPQGVRTTLSVPSRVGSESEDEDLLSELELADRDGQPGCTTATCPIQGAPDSGKCSASSCLDTSNDPDDLDVLRTRHARHTRKRRRLV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Bovine

Sheep

Dog

Pig

Chicken

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5SXM2 As Substrate

Q5SXM2

Site	PTM Type	Source
S68	Phosphorylation	Uniprot
S135	Phosphorylation	Uniprot
S224	Phosphorylation	Uniprot
K240	Ubiquitination	Uniprot
K411	Ubiquitination	Uniprot
S450	Phosphorylation	Uniprot
K501	Acetylation	Uniprot
T521	Phosphorylation	Uniprot
S524	Phosphorylation	Uniprot
S528	Phosphorylation	Uniprot
S531	Phosphorylation	Uniprot
S534	Phosphorylation	Uniprot
S535	Phosphorylation	Uniprot
S536	Phosphorylation	Uniprot
S537	Phosphorylation	Uniprot
S538	Phosphorylation	Uniprot
S539	Phosphorylation	Uniprot
S541	Phosphorylation	Uniprot
S561	Phosphorylation	Uniprot
S597	Phosphorylation	Uniprot
S599	Phosphorylation	Uniprot
S604	Phosphorylation	Uniprot
S605	Phosphorylation	Uniprot
S611	Phosphorylation	Uniprot
S626	Phosphorylation	Uniprot
K657	Ubiquitination	Uniprot
S702	Phosphorylation	Uniprot
T1150	Phosphorylation	Uniprot
T1157	Phosphorylation	Uniprot
S1163	Phosphorylation	Uniprot
T1221	Phosphorylation	Uniprot
S1224	Phosphorylation	Uniprot
K1256	Acetylation	Uniprot
R1300	Methylation	Uniprot
S1315	Phosphorylation	Uniprot
S1391	Phosphorylation	Uniprot
S1398	Phosphorylation	Uniprot
S1400	Phosphorylation	Uniprot
S1407	Phosphorylation	Uniprot

Research Backgrounds

Q5SXM2_SNPC4_HUMAN

Function:

Part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes. Binds to the proximal sequence element (PSE), a non-TATA-box basal promoter element common to these 2 types of genes. Recruits TBP and BRF2 to the U6 snRNA TATA box.

Subcellular Location:

Nucleus.

Subunit Structure:

Part of the SNAPc complex composed of 5 subunits: SNAPC1, SNAPC2, SNAPC3, SNAPC4 and SNAPC5. SNAPC4 interacts with SNAPC1, SNAPC2, SNAPC5, BRF2 and TBP.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

SNAPC4 Antibody - #DF2405