POGZ Antibody - #DF2419

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Product: POGZ Antibody
Catalog: DF2419
Description: Rabbit polyclonal antibody to POGZ
Application: WB IHC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 155 kDa; 155kD(Calculated).
Uniprot: Q7Z3K3
RRID: AB_2839625

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
POGZ Antibody detects endogenous levels of total POGZ.
RRID:
AB_2839625
Cite Format: Affinity Biosciences Cat# DF2419, RRID:AB_2839625.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KIAA0461; MGC71543; Pogo transposable element with ZNF domain; POGZ; POGZ_HUMAN; putative protein product of Nbla00003; SUHW5; suppressor of hairy wing homolog; Suppressor of hairy wing homolog 5; Zinc finger protein 280E; Zinc finger protein 635; ZNF280E; ZNF635; ZNF635M;

Immunogens

Immunogen:
Uniprot:

Q7Z3K3(POGZ_HUMAN) >>Visit HPA database.

Gene(ID):
POGZ(23126)
Description:
Plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms.
Sequence:
MADTDLFMECEEEELEPWQKISDVIEDSVVEDYNSVDKTTTVSVSQQPVSAPVPIAAHASVAGHLSTSTTVSSSGAQNSDSTKKTLVTLIANNNAGNPLVQQGGQPLILTQNPAPGLGTMVTQPVLRPVQVMQNANHVTSSPVASQPIFITTQGFPVRNVRPVQNAMNQVGIVLNVQQGQTVRPITLVPAPGTQFVKPTVGVPQVFSQMTPVRPGSTMPVRPTTNTFTTVIPATLTIRSTVPQSQSQQTKSTPSTSTTPTATQPTSLGQLAVQSPGQSNQTTNPKLAPSFPSPPAVSIASFVTVKRPGVTGENSNEVAKLVNTLNTIPSLGQSPGPVVVSNNSSAHGSQRTSGPESSMKVTSSIPVFDLQDGGRKICPRCNAQFRVTEALRGHMCYCCPEMVEYQKKGKSLDSEPSVPSAAKPPSPEKTAPVASTPSSTPIPALSPPTKVPEPNENVGDAVQTKLIMLVDDFYYGRDGGKVAQLTNFPKVATSFRCPHCTKRLKNNIRFMNHMKHHVELDQQNGEVDGHTICQHCYRQFSTPFQLQCHLENVHSPYESTTKCKICEWAFESEPLFLQHMKDTHKPGEMPYVCQVCQYRSSLYSEVDVHFRMIHEDTRHLLCPYCLKVFKNGNAFQQHYMRHQKRNVYHCNKCRLQFLFAKDKIEHKLQHHKTFRKPKQLEGLKPGTKVTIRASRGQPRTVPVSSNDTPPSALQEAAPLTSSMDPLPVFLYPPVQRSIQKRAVRKMSVMGRQTCLECSFEIPDFPNHFPTYVHCSLCRYSTCCSRAYANHMINNHVPRKSPKYLALFKNSVSGIKLACTSCTFVTSVGDAMAKHLVFNPSHRSSSILPRGLTWIAHSRHGQTRDRVHDRNVKNMYPPPSFPTNKAATVKSAGATPAEPEELLTPLAPALPSPASTATPPPTPTHPQALALPPLATEGAECLNVDDQDEGSPVTQEPELASGGGGSGGVGKKEQLSVKKLRVVLFALCCNTEQAAEHFRNPQRRIRRWLRRFQASQGENLEGKYLSFEAEEKLAEWVLTQREQQLPVNEETLFQKATKIGRSLEGGFKISYEWAVRFMLRHHLTPHARRAVAHTLPKDVAENAGLFIDFVQRQIHNQDLPLSMIVAIDEISLFLDTEVLSSDDRKENALQTVGTGEPWCDVVLAILADGTVLPTLVFYRGQMDQPANMPDSILLEAKESGYSDDEIMELWSTRVWQKHTACQRSKGMLVMDCHRTHLSEEVLAMLSASSTLPAVVPAGCSSKIQPLDVCIKRTVKNFLHKKWKEQAREMADTACDSDVLLQLVLVWLGEVLGVIGDCPELVQRSFLVASVLPGPDGNINSPTRNADMQEELIASLEEQLKLSGEHSESSTPRPRSSPEETIEPESLHQLFEGESETESFYGFEEADLDLMEI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7Z3K3 As Substrate

Site PTM Type Enzyme
S28 Phosphorylation
Y33 Phosphorylation
S35 Phosphorylation
R183 Methylation
S244 O-Glycosylation
S251 Phosphorylation
T258 Phosphorylation
S274 Phosphorylation
S278 Phosphorylation
T281 Phosphorylation
T282 Phosphorylation
S292 Phosphorylation
S314 Phosphorylation
K319 Ubiquitination
S333 Phosphorylation
S340 Phosphorylation
S344 Phosphorylation
S352 Phosphorylation
S363 Phosphorylation
S410 Phosphorylation
S416 Phosphorylation
S419 Phosphorylation
S425 Phosphorylation
T429 Phosphorylation
S434 Phosphorylation
T435 Phosphorylation
S437 Phosphorylation
T439 Phosphorylation
S445 Phosphorylation
T448 Phosphorylation
T463 Phosphorylation
K480 Ubiquitination
K489 Ubiquitination
T492 Phosphorylation
S493 Phosphorylation
K666 Ubiquitination
K671 Ubiquitination
K677 Ubiquitination
K683 Ubiquitination
T686 Phosphorylation
T689 Phosphorylation
S704 Phosphorylation
T707 Phosphorylation
S746 Phosphorylation
T780 Phosphorylation
S799 Phosphorylation
K801 Acetylation
K801 Sumoylation
K801 Ubiquitination
S856 Phosphorylation
K883 Acetylation
S949 Phosphorylation
S964 Phosphorylation
S1013 Phosphorylation
K1030 Ubiquitination
K1053 Ubiquitination
T1082 Phosphorylation
S1200 Phosphorylation
K1215 Ubiquitination
K1223 Ubiquitination
K1269 Ubiquitination
S1327 Phosphorylation
S1338 Phosphorylation
T1340 Phosphorylation
S1352 Phosphorylation
S1360 Phosphorylation
S1364 Phosphorylation
S1366 Phosphorylation
S1367 Phosphorylation
T1368 Phosphorylation
S1392 Phosphorylation

Research Backgrounds

Function:

Plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms.

PTMs:

Phosphorylation increases its interaction with PSIP1.

Subcellular Location:

Nucleus. Chromosome. Cytoplasm.
Note: According to some authors, it is not localized to mitotic chromatin (PubMed:19244240). Recruited to trimethylated 'Lys-9' of histone H3 (H3K9me3).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with CBX1, CBX3, MAD2L2 and CHAMP1. Interacts with CBX5; POGZ competes with PXVXL motif-containing proteins such as INCENP and TRIM28 for interaction with CBX5. Interacts (via IBM motif) with PSIP1 isoform 1 (via IBD domain); phosphorylation increases its affinity for PSIP1.

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