TOPRS Antibody - #DF2475

(2)  
Product: TOPRS Antibody
Catalog: DF2475
Description: Rabbit polyclonal antibody to TOPRS
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 119kDa; 119kD(Calculated).
Uniprot: Q9NS56
RRID: AB_2839681

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(80%), Bovine(100%), Horse(80%), Sheep(100%), Rabbit(100%), Dog(90%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
TOPRS Antibody detects endogenous levels of total TOPRS.
RRID:
AB_2839681
Cite Format: Affinity Biosciences Cat# DF2475, RRID:AB_2839681.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AW105885; E3 ubiquitin protein ligase Topors; E3 ubiquitin-protein ligase Topors; LUN; p53 binding protein 3; p53 BP3/LUN; p53-binding protein 3; p53BP3; p53BP3/LUN; Retinitis pigmentosa 31; RP31; SUMO1 protein E3 ligase Topors; SUMO1-protein E3 ligase Topors; Topoisomerase I binding arginine/serine rich; Topoisomerase I binding arginine/serine rich protein; Topoisomerase I binding protein; Topoisomerase I binding RING finger protein; Topoisomerase I-binding arginine/serine-rich protein; Topoisomerase I-binding RING finger protein; Topoisomerase I-binding RS protein; Topors; TOPRS_HUMAN; TP53BPL; Tumor protein p53 binding protein; Tumor suppressor p53 binding protein 3; Tumor suppressor p53-binding protein 3;

Immunogens

Immunogen:
Uniprot:

Q9NS56(TOPRS_HUMAN) >>Visit HPA database.

Gene(ID):
TOPORS(10210)
Expression:
Q9NS56 TOPRS_HUMAN:

Expressed at highest levels in testis and at lower levels in adrenal gland, bone marrow, brain, colon, heart, kidney, liver, muscle, ovary, pancreas, placenta, prostate, skeletal muscle, skin, small intestine, spleen, stomach, testis, thymus, thyroid and uterus. Expressed in the alveolar epithelium of the lung. Expression is commonly decreased in colon adenocarcinomas and lung cancers.

Sequence:
MGSQPPLGSPLSREEGEAPPPAPASEGRRRSRRVRLRGSCRHRPSFLGCRELAASAPARPAPASSEIMASAAKEFKMDNFSPKAGTSKLQQTVPADASPDSKCPICLDRFDNVSYLDRCLHKFCFRCVQEWSKNKAECPLCKQPFDSIFHSVRAEDDFKEYVLRPSYNGSFVTPDRRFRYRTTLTRERNASVYSPSGPVNRRTTTPPDSGVLFEGLGISTRPRDVEIPQFMRQIAVRRPTTADERSLRKIQEQDIINFRRTLYRAGARVRNIEDGGRYRDISAEFFRRNPACLHRLVPWLKRELTVLFGAHGSLVNIVQHIIMSNVTRYDLESQAFVSDLRPFLLNRTEHFIHEFISFARSPFNMAAFDQHANYDCPAPSYEEGSHSDSSVITISPDEAETQELDINVATVSQAPWDDETPGPSYSSSEQVHVTMSSLLNTSDSSDEELVTGGATSQIQGVQTNDDLNNDSDDSSDNCVIVGFVKPLAERTPELVELSSDSEDLGSYEKMETVKTQEQEQSYSSGDSDVSRCSSPHSVLGKDEQINKGHCDSSTRIKSKKEEKRSTSLSSPRNLNSSVRGDRVYSPYNHRHRKRGRSRSSDSRSQSRSGHDQKNHRKHHGKKRMKSKRSRSRESSRPRGRRDKKRSRTRDSSWSRRSQTLSLSSESTSRSRSRSSDHGKRRSRSRNRDRYYLRNNYGSRYKWEYTYYSRNKDRDGYESSYRRRTLSRAHYSRQSSSPEFRVQSFSERTNARKKNNHSERKYYYYERHRSRSLSSNRSRTASTGTDRVRNEKPGGKRKYKTRHLEGTNEVAQPSREFASKAKDSHYQKSSSKLDGNYKNESDTFSDSRSSDRETKHKRRKRKTRSLSVEIVYEGKATDTTKHHKKKKKKHKKKHKKHHGDNASRSPVVITIDSDSDKDSEVKEDTECDNSGPQDPLQNEFLAPSLEPFETKDVVTIEAEFGVLDKECDIATLSNNLNNANKTVDNIPPLAASVEQTLDVREESTFVSDLENQPSNIVSLQTEPSRQLPSPRTSLMSVCLGRDCDMS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Rabbit
100
Dog
90
Chicken
88
Pig
80
Horse
80
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NS56 As Substrate

Site PTM Type Enzyme
S3 Phosphorylation
S9 Phosphorylation
S12 Phosphorylation
R28 Methylation
S45 Phosphorylation
K73 Ubiquitination
K76 Sumoylation
K76 Ubiquitination
S81 Phosphorylation
K83 Sumoylation
K83 Ubiquitination
K88 Sumoylation
K88 Ubiquitination
S98 Phosphorylation
S101 Phosphorylation
K102 Ubiquitination
K122 Ubiquitination
K135 Ubiquitination
T173 Phosphorylation
Y180 Phosphorylation
T183 Phosphorylation
S191 Phosphorylation
Y193 Phosphorylation
S194 Phosphorylation
S196 Phosphorylation
T204 Phosphorylation
T205 Phosphorylation
K249 Sumoylation
K249 Ubiquitination
Y263 Phosphorylation
T491 Phosphorylation
S498 Phosphorylation
S499 Phosphorylation
S501 Phosphorylation
S533 Phosphorylation
S534 Phosphorylation
S537 Phosphorylation
K547 Sumoylation
K547 Ubiquitination
K557 Acetylation
K559 Acetylation
K560 Acetylation
K560 Sumoylation
S567 Phosphorylation
S569 Phosphorylation
S570 Phosphorylation
R572 Methylation
S576 Phosphorylation
S577 Phosphorylation
R579 Methylation
R582 Methylation
Y584 Phosphorylation
S585 Phosphorylation
Y587 Phosphorylation
K679 Acetylation
K701 Sumoylation
Y707 Phosphorylation
S708 Phosphorylation
K711 Sumoylation
K711 Ubiquitination
S718 Phosphorylation P53350 (PLK1)
S731 Phosphorylation
S734 Phosphorylation
S735 Phosphorylation
S736 Phosphorylation
K760 Ubiquitination
S781 Phosphorylation
T784 Phosphorylation
K791 Ubiquitination
K799 Ubiquitination
K819 Ubiquitination
K821 Ubiquitination
K827 Ubiquitination
K831 Ubiquitination
T862 Phosphorylation
S864 Phosphorylation
S866 Phosphorylation
S902 Phosphorylation
S912 Phosphorylation
S914 Phosphorylation
S918 Phosphorylation
S1028 Phosphorylation

Research Backgrounds

Function:

Functions as an E3 ubiquitin-protein ligase and as an E3 SUMO1-protein ligase. Probable tumor suppressor involved in cell growth, cell proliferation and apoptosis that regulates p53/TP53 stability through ubiquitin-dependent degradation. May regulate chromatin modification through sumoylation of several chromatin modification-associated proteins. May be involved in DNA damage-induced cell death through IKBKE sumoylation.

PTMs:

Phosphorylation at Ser-98 regulates the E3 ubiquitin-protein ligase activity but not the SUMO1-protein ligase activity. Phosphorylation at Ser-718 increases the E3 ubiquitin-protein ligase activity versus the SUMO1-protein ligase activity resulting in increased p53/TP53 ubiquitination and degradation.

Sumoylated.

Subcellular Location:

Nucleus. Nucleus>PML body.
Note: Localizes to discrete nuclear foci which partly overlap with PML nuclear bodies. Targeted to PML nuclear bodies upon DNA damage.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at highest levels in testis and at lower levels in adrenal gland, bone marrow, brain, colon, heart, kidney, liver, muscle, ovary, pancreas, placenta, prostate, skeletal muscle, skin, small intestine, spleen, stomach, testis, thymus, thyroid and uterus. Expressed in the alveolar epithelium of the lung. Expression is commonly decreased in colon adenocarcinomas and lung cancers.

Subunit Structure:

Interacts with PARK7/DJ-1 (By similarity). Interacts with TOP1. Interacts with p53/TP53; can both ubiquitinate and sumoylate p53/TP53. Interacts with the SUMO1 conjugating enzyme UBE2I. Interacts with SUMO1. Interacts with NKX3-1; polyubiquitinates NKX3-1 and induces its proteasomal degradation. Interacts with SIN3A; sumoylates SIN3A. Interacts with IKBKE; induced by DNA damage.

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