DNAJB6 Antibody - #DF2489
| Product: | DNAJB6 Antibody |
| Catalog: | DF2489 |
| Description: | Rabbit polyclonal antibody to DNAJB6 |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Rabbit, Dog |
| Mol.Wt.: | 36 kDa; 36kD(Calculated). |
| Uniprot: | O75190 |
| RRID: | AB_2839695 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF2489, RRID:AB_2839695.
Fold/Unfold
DJ4; DnaJ; DnaJ (Hsp40) homolog subfamily B member 6; DNAJ B6; DnaJ homolog subfamily B member 6; DnaJ like 2 protein; DNAJB6; DNJB6_HUMAN; Heat shock protein J2; HHDJ1; HSJ-2; HSJ2; LGMD1E; MRJ; MSJ-1; MSJ1;
Immunogens
Widely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus.
- O75190 DNJB6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVDYYEVLGVQRHASPEDIKKAYRKLALKWHPDKNPENKEEAERKFKQVAEAYEVLSDAKKRDIYDKYGKEGLNGGGGGGSHFDSPFEFGFTFRNPDDVFREFFGGRDPFSFDFFEDPFEDFFGNRRGPRGSRSRGTGSFFSAFSGFPSFGSGFSSFDTGFTSFGSLGHGGLTSFSSTSFGGSGMGNFKSISTSTKMVNGRKITTKRIVENGQERVEVEEDGQLKSLTINGVADDDALAEERMRRGQNALPAQPAGLRPPKPPRPASLLRHAPHCLSEEEGEQDRPRAPGPWDPLASAAGLKEGGKRKKQKQREESKKKKSTKGNH
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O75190 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y4 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| Y53 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| Y65 | Phosphorylation | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| R135 | Methylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| T228 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| K302 | Acetylation | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| K306 | Acetylation | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| S316 | Phosphorylation | Uniprot |
Research Backgrounds
Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity.
Cytoplasm>Perinuclear region. Nucleus. Cytoplasm>Myofibril>Sarcomere>Z line.
Widely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus.
Homooligomer. Interacts with BAG3, HSPB8 and STUB1. Interacts with ALKBH1 (By similarity). Interacts with HSP70, KRT18 and PTTG. Isoform B interacts with histone deacetylases HDAC4, HDAC6, and SIRT2, HDAC activity is required for antiaggregation.
The antiaggregation activity of isoform B resides in the serine-rich region and the C-terminus.
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