FGFP1 Antibody | Affinity Biosciences

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Product:	FGFP1 Antibody
Catalog:	DF2498
Description:	Rabbit polyclonal antibody to FGFP1
Application:	WB IHC
Reactivity:	Human
Prediction:	Bovine
Mol.Wt.:	26kDa; 26kD(Calculated).
Uniprot:	Q14512
RRID:	AB_2839704

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Bovine(86%)

Clonality:

Polyclonal

Specificity:

FGFP1 Antibody detects endogenous levels of total FGFP1.

RRID:

AB_2839704
Cite Format: Affinity Biosciences Cat# DF2498, RRID:AB_2839704.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

17 kDa HBGF-binding protein; 17 kDa heparin binding growth factor binding protein; 17 kDa heparin-binding growth factor-binding protein; FGF binding protein 1; FGF BP; FGF BP1; FGF-binding protein 1; FGF-BP; FGF-BP1; FGFBP; FGFBP-1; Fgfbp1; FGFP1_HUMAN; Fibroblast growth factor binding protein 1 precursor; Fibroblast growth factor-binding protein 1; HBp17;

Immunogens

Immunogen:

Uniprot:

Q14512(FGFP1_HUMAN) >>Visit HPA database.

Gene(ID):

FGFBP1(9982)

Expression:

Q14512 FGFP1_HUMAN:

Expressed in the suprabasal region of the epidermis, in hair follicles, the basement membrane at the dermo-epidermal junction (occasionally extending into the basement membrane of dermal blood vessels), wounded skin and several invasive squamous cell carcinomas (at protein level). Expressed in normal and wounded skin and various squamous cell carcinomas.

Sequence:

Q14512 FGFP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKICSLTLLSFLLLAAQVLLVEGKKKVKNGLHSKVVSEQKDTLGNTQIKQKSRPGNKGKFVTKDQANCRWAATEQEEGISLKVECTQLDHEFSCVFAGNPTSCLKLKDERVYWKQVARNLRSQKDICRYSKTAVKTRVCRKDFPESSLKLVSSTLFGNTKPRKEKTEMSPREHIKGKETTPSSLAVTQTMATKAPECVEDPDMANQRKTALEFCGETWSSLCTFFLSIVQDTSC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Pig

Horse

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14512 As Substrate

Q14512

Site	PTM Type	Source
S37	O-Glycosylation	Uniprot
T46	O-Glycosylation	Uniprot
K57	Acetylation	Uniprot
T73	O-Glycosylation	Uniprot
S80	Phosphorylation	Uniprot
T179	O-Glycosylation	Uniprot
T180	O-Glycosylation	Uniprot
S182	O-Glycosylation	Uniprot
T187	O-Glycosylation	Uniprot
T189	O-Glycosylation	Uniprot
T192	O-Glycosylation	Uniprot

Research Backgrounds

Q14512_FGFP1_HUMAN

Function:

Acts as a carrier protein that release fibroblast-binding factors (FGFs) from the extracellular matrix (EM) storage and thus enhance the mitogenic activity of FGFs. Enhances FGF2 signaling during tissue repair, angiogenesis and in tumor growth.

Subcellular Location:

Secreted>Extracellular space. Cell membrane>Peripheral membrane protein.
Note: Extracellular and plasma membrane-associated. Colocalizes with HSPG2 in the pericellular environment of squamous cell carcinomas.

Tissue Specificity:

Subunit Structure:

Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGF1, FGF2, FGF7, FGF10, FGF22 and HSPG2.

Family&Domains:

Belongs to the fibroblast growth factor-binding protein family.

References

1). FGFBP1-mediated crosstalk between fibroblasts and pancreatic cancer cells via FGF22/FGFR2 promotes invasion and metastasis of pancreatic cancer. ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2021 (PubMed: 34117747) [IF=3.3]

Application: WB Species: Human Sample: PANC-1 and Mia PaCa-2 cells

Figure 1. FGFBP1 silencing in PCCs decreased the expression and secretion of FGF22 in human CAFs cocultured with PCCs (A) Efficiency of FGFBP1 knockdown in PANC-1 and Mia PaCa-2 cells evaluated by qRT-PCR. (B) Efficiency of FGFBP1 knockdown in PANC-1 and Mia PaCa-2 cells evaluated by western blot analysis. (C) Heat map of differentially expressed genes in mouse fibroblasts cocultured with FGFBP1-shRNA-transfected PANC-1 cells compared with mouse fibroblasts cocultured with scramble-transfected PANC-1 cells. (D) mRNA level of FGF22 in human CAFs cocultured with FGFBP1-shRNA-transfected PANC-1 or Mia PaCa-2 cells (CAF-P or CAF-M) compared with that in CAFs cocultured with scramble-transfected PANC-1 or Mia PaCa-2 cells. (E) Protein level of FGF22 in CAF-P or CAF-M compared with that in CAFs cocultured with scramble-transfected PANC-1 or Mia PaCa-2 cells are shown. (F) Secretion of FGF22 in culture medium of CAF-P or CAF-M compared with that in culture medium of CAFs cocultured with scramble-transfected PANC-1 or Mia PaCa-2 cells.

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FGFP1 Antibody - #DF2498