HSPA12B Antibody - #DF2502
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| Product: | HSPA12B Antibody |
| Catalog: | DF2502 |
| Description: | Rabbit polyclonal antibody to HSPA12B |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 76 kDa; 76kD(Calculated). |
| Uniprot: | Q96MM6 |
| RRID: | AB_2839708 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Pig(92%), Bovine(83%), Horse(83%), Sheep(83%), Rabbit(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
HSPA12B Antibody detects endogenous levels of total HSPA12B.
RRID:
AB_2839708
Cite Format: Affinity Biosciences Cat# DF2502, RRID:AB_2839708.
Cite Format: Affinity Biosciences Cat# DF2502, RRID:AB_2839708.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Heat shock 70 kDa protein 12B; HS12B_HUMAN; Hspa12b;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q96MM6 HS12B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLAVPEMGLQGLYIGSSPERSPVPSPPGSPRTQESCGIAPLTPSQSPKPEVRAPQQASFSVVVAIDFGTTSSGYAFSFASDPEAIHMMRKWEGGDPGVAHQKTPTCLLLTPEGAFHSFGYTARDYYHDLDPEEARDWLYFEKFKMKIHSATDLTLKTQLEAVNGKTMPALEVFAHALRFFREHALQELREQSPSLPEKDTVRWVLTVPAIWKQPAKQFMREAAYLAGLVSRENAEQLLIALEPEAASVYCRKLRLHQLLDLSGRAPGGGRLGERRSIDSSFRQAREQLRRSRHSRTFLVESGVGELWAEMQAGDRYVVADCGGGTVDLTVHQLEQPHGTLKELYKASGGPYGAVGVDLAFEQLLCRIFGEDFIATFKRQRPAAWVDLTIAFEARKRTAGPHRAGALNISLPFSFIDFYRKQRGHNVETALRRSSVNFVKWSSQGMLRMSCEAMNELFQPTVSGIIQHIEALLARPEVQGVKLLFLVGGFAESAVLQHAVQAALGARGLRVVVPHDVGLTILKGAVLFGQAPGVVRVRRSPLTYGVGVLNRFVPGRHPPEKLLVRDGRRWCTDVFERFVAAEQSVALGEEVRRSYCPARPGQRRVLINLYCCAAEDARFITDPGVRKCGALSLELEPADCGQDTAGAPPGRREIRAAMQFGDTEIKVTAVDVSTNRSVRASIDFLSN
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
92
Dog
92
Rabbit
92
Horse
83
Bovine
83
Sheep
83
Zebrafish
70
Xenopus
64
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96MM6 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S25 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| T42 | Phosphorylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| T200 | Phosphorylation | Uniprot | |
| T206 | Phosphorylation | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| S280 | Phosphorylation | Uniprot | |
| T397 | Phosphorylation | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| T667 | Phosphorylation | Uniprot |
Research Backgrounds
Tissue Specificity:
Highest expression in muscle and heart. Lower levels in liver and kidney.
Family&Domains:
Belongs to the heat shock protein 70 family.
References
1). Landscape of tissue-specific RNA Editome provides insight into co-regulated and altered gene expression in pigs (Sus-scrofa). RNA Biology, 2021
(PubMed: 34314293)
[IF=3.6]
Application: WB Species: Human Sample: MDS cells
Figure 5. The role of HSPA12B in MDS cell proliferation. (A-B) The mRNA expression level of HSPA12B in MDS cells after si-HSPA12B and HSPA12B-OE transfection. (C)
The protein expression level of HSPA12B was accessed by western blot using GAPDH protein and PCNA (proliferation marker) as an internal control after si-HSPA12B
transfection. Data collected from three independently replicated wells. (D) The HSPA12B protein expression level accessed by western blot using GAPDH protein as
an internal control after HSPA12B-OE transfection. Data collected from three independently replicated wells. (E-F) The CCK-8 assay displayed absorbance at 450 nm in
MDS cells after si-HSPA12B and HSPA12B-OE transfection. Data were collected and calculated as mean ± SD from three independently replicated wells. (G-H) The EdU
positive cells in red after si-HSPA12B and HSPA12B-OE transfection. Nuclei were stained with DAPI (blue). Data were collected from three independently replicated
wells. Scale bar, 100 µm. Significance in (A-B) and (E-F): **P < 0.01.
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