IL16 Antibody - #DF2522
(1)
(3)
| Product: | IL16 Antibody |
| Catalog: | DF2522 |
| Description: | Rabbit polyclonal antibody to IL16 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 142 kDa; 142kD(Calculated). |
| Uniprot: | Q14005 |
| RRID: | AB_2839728 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF2522, RRID:AB_2839728.
Fold/Unfold
HGNC:5980; HsT19289; IL-16; IL16; IL16_HUMAN; Interleukin 16 precursor; Interleukin-16; LCF; Lymphocyte chemoattractant factor; Neuronal interleukin 16; NIL16; prIL 16; PrIL16; Prointerleukin 16;
Immunogens
Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T-cell proliferation.
- Q14005 IL16_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESHSRAGKSRKSAKFRSISRSLMLCNAKTSDDGSSPDEKYPDPFEISLAQGKEGIFHSSVQLADTSEAGPSSVPDLALASEAAQLQAAGNDRGKTCRRIFFMKESSTASSREKPGKLEAQSSNFLFPKACHQRARSNSTSVNPYCTREIDFPMTKKSAAPTDRQPYSLCSNRKSLSQQLDCPAGKAAGTSRPTRSLSTAQLVQPSGGLQASVISNIVLMKGQAKGLGFSIVGGKDSIYGPIGIYVKTIFAGGAAAADGRLQEGDEILELNGESMAGLTHQDALQKFKQAKKGLLTLTVRTRLTAPPSLCSHLSPPLCRSLSSSTCITKDSSSFALESPSAPISTAKPNYRIMVEVSLQKEAGVGLGIGLCSVPYFQCISGIFVHTLSPGSVAHLDGRLRCGDEIVEISDSPVHCLTLNEVYTILSHCDPGPVPIIVSRHPDPQVSEQQLKEAVAQAVENTKFGKERHQWSLEGVKRLESSWHGRPTLEKEREKNSAPPHRRAQKVMIRSSSDSSYMSGSPGGSPGSGSAEKPSSDVDISTHSPSLPLAREPVVLSIASSRLPQESPPLPESRDSHPPLRLKKSFEILVRKPMSSKPKPPPRKYFKSDSDPQKSLEERENSSCSSGHTPPTCGQEARELLPLLLPQEDTAGRSPSASAGCPGPGIGPQTKSSTEGEPGWRRASPVTQTSPIKHPLLKRQARMDYSFDTTAEDPWVRISDCIKNLFSPIMSENHGHMPLQPNASLNEEEGTQGHPDGTPPKLDTANGTPKVYKSADSSTVKKGPPVAPKPAWFRQSLKGLRNRASDPRGLPDPALSTQPAPASREHLGSHIRASSSSSSIRQRISSFETFGSSQLPDKGAQRLSLQPSSGEAAKPLGKHEEGRFSGLLGRGAAPTLVPQQPEQVLSSGSPAASEARDPGVSESPPPGRQPNQKTLPPGPDPLLRLLSTQAEESQGPVLKMPSQRARSFPLTRSQSCETKLLDEKTSKLYSISSQVSSAVMKSLLCLPSSISCAQTPCIPKEGASPTSSSNEDSAANGSAETSALDTGFSLNLSELREYTEGLTEAKEDDDGDHSSLQSGQSVISLLSSEELKKLIEEVKVLDEATLKQLDGIHVTILHKEEGAGLGFSLAGGADLENKVITVHRVFPNGLASQEGTIQKGNEVLSINGKSLKGTTHHDALAILRQAREPRQAVIVTRKLTPEAMPDLNSSTDSAASASAASDVSVESTAEATVCTVTLEKMSAGLGFSLEGGKGSLHGDKPLTINRIFKGAASEQSETVQPGDEILQLGGTAMQGLTRFEAWNIIKALPDGPVTIVIRRKSLQSKETTAAGDS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14005 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S139 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| T298 | Phosphorylation | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| T325 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| S481 | Phosphorylation | Uniprot | |
| S566 | Phosphorylation | Uniprot | |
| S572 | Phosphorylation | Uniprot | |
| S584 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| T631 | Phosphorylation | Uniprot | |
| S683 | Phosphorylation | Uniprot | |
| S705 | Phosphorylation | Uniprot | |
| S743 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| T757 | Phosphorylation | P06493 (CDK1) | Uniprot |
| Y771 | Phosphorylation | Uniprot | |
| K772 | Ubiquitination | Uniprot | |
| K788 | Ubiquitination | Uniprot | |
| S795 | Phosphorylation | Uniprot | |
| S804 | Phosphorylation | Uniprot | |
| S833 | Phosphorylation | Uniprot | |
| S834 | Phosphorylation | Uniprot | |
| S835 | Phosphorylation | Uniprot | |
| S836 | Phosphorylation | Uniprot | |
| S844 | Phosphorylation | Uniprot | |
| S845 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| T848 | Phosphorylation | Uniprot | |
| S851 | Phosphorylation | Uniprot | |
| S852 | Phosphorylation | Uniprot | |
| K857 | Ubiquitination | Uniprot | |
| S863 | Phosphorylation | Uniprot | |
| R882 | Methylation | Uniprot | |
| S884 | Phosphorylation | Uniprot | |
| R889 | Methylation | Uniprot | |
| S920 | Phosphorylation | Uniprot | |
| S922 | Phosphorylation | Uniprot | |
| T933 | Phosphorylation | Uniprot | |
| S946 | Phosphorylation | Uniprot | |
| T947 | Phosphorylation | Uniprot | |
| S952 | Phosphorylation | Uniprot | |
| S961 | Phosphorylation | Uniprot | |
| S966 | Phosphorylation | Uniprot | |
| T970 | Phosphorylation | Uniprot | |
| S972 | Phosphorylation | Uniprot | |
| S974 | Phosphorylation | Uniprot | |
| K978 | Methylation | Uniprot | |
| S989 | Phosphorylation | Uniprot | |
| S995 | Phosphorylation | Uniprot | |
| S996 | Phosphorylation | Uniprot | |
| S1001 | Phosphorylation | Uniprot | |
| S1074 | Phosphorylation | Uniprot | |
| S1247 | Phosphorylation | Uniprot | |
| S1254 | Phosphorylation | Uniprot | |
| K1259 | Ubiquitination | Uniprot | |
| K1305 | Ubiquitination | Uniprot | |
| S1320 | Phosphorylation | Uniprot | |
| K1324 | Ubiquitination | Uniprot |
Research Backgrounds
Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16.
Secreted.
Cytoplasm.
Cytoplasm. Nucleus.
Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T-cell proliferation.
Homotetramer (Probable). According to the formation of a homotetrameric protein complex is not required for the chemo-attractant function. Isoform 3 interacts (via PDZ 3 domain) with PPP1R12A, PPP1R12B and PPP1R12C. Isoform 1 interacts with PPP1R12B. Isoform 3 interacts with GRIN2A. Isoform 3 interacts with GABPB1. Isoform 3 interacts (via PDZ 3 domain) with HDAC3. Isoform 1 interacts with GRIN2D, KCNJ10, KCNJ15 and CACNA1C (By similarity).
(Microbial infection) Isoform 3 interacts with HTLV-1 tax.
References
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