ST2 Antibody - #DF2533

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Product: ST2 Antibody
Catalog: DF2533
Description: Rabbit polyclonal antibody to ST2
Application: WB IHC
Reactivity: Human, Mouse
Mol.Wt.: 70 kDa; 63kD(Calculated).
Uniprot: Q01638
RRID: AB_2839739

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
ST2 Antibody detects endogenous levels of total ST2.
RRID:
AB_2839739
Cite Format: Affinity Biosciences Cat# DF2533, RRID:AB_2839739.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DER-4; DER4; FIT 1; Growth stimulation expressed; homolog of mouse growth stimulation-expressed; Il1rl1; IL33R; ILRL1_HUMAN; Interleukin 1 receptor like 1; interleukin 1 receptor related protein; Interleukin-1 receptor-like 1; Protein ST2; ST2; ST2L; ST2V; T1; T1 protein;

Immunogens

Immunogen:
Uniprot:

Q01638(ILRL1_HUMAN) >>Visit HPA database.

Gene(ID):
IL1RL1(9173)
Expression:
Q01638 ILRL1_HUMAN:

Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle. Expressed on T-cells in fibrotic liver; at protein level. Overexpressed in fibrotic and cirrhotic liver.

Description:
Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function.
Sequence:
MGFWILAILTILMYSTAAKFSKQSWGLENEALIVRCPRQGKPSYTVDWYYSQTNKSIPTQERNRVFASGQLLKFLPAAVADSGIYTCIVRSPTFNRTGYANVTIYKKQSDCNVPDYLMYSTVSGSEKNSKIYCPTIDLYNWTAPLEWFKNCQALQGSRYRAHKSFLVIDNVMTEDAGDYTCKFIHNENGANYSVTATRSFTVKDEQGFSLFPVIGAPAQNEIKEVEIGKNANLTCSACFGKGTQFLAAVLWQLNGTKITDFGEPRIQQEEGQNQSFSNGLACLDMVLRIADVKEEDLLLQYDCLALNLHGLRRHTVRLSRKNPIDHHSIYCIIAVCSVFLMLINVLVIILKMFWIEATLLWRDIAKPYKTRNDGKLYDAYVVYPRNYKSSTDGASRVEHFVHQILPDVLENKCGYTLCIYGRDMLPGEDVVTAVETNIRKSRRHIFILTPQITHNKEFAYEQEVALHCALIQNDAKVILIEMEALSELDMLQAEALQDSLQHLMKVQGTIKWREDHIANKRSLNSKFWKHVRYQMPVPSKIPRKASSLTPLAAQKQ

PTMs - Q01638 As Substrate

Site PTM Type Enzyme
N95 N-Glycosylation
N140 N-Glycosylation
T142 Phosphorylation
S164 Phosphorylation
Y179 Phosphorylation
N191 N-Glycosylation
S193 Phosphorylation
T195 Phosphorylation
T197 Phosphorylation
T370 Phosphorylation
Y377 Phosphorylation
Y383 Phosphorylation
Y387 Phosphorylation
S525 Phosphorylation
Y533 Phosphorylation
S539 Phosphorylation
S546 Phosphorylation

Research Backgrounds

Function:

Receptor for interleukin-33 (IL-33); signaling requires association of the coreceptor IL1RAP. Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function.

Inhibits IL-33 signaling.

Subcellular Location:

Cell membrane.

Secreted.

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle. Expressed on T-cells in fibrotic liver; at protein level. Overexpressed in fibrotic and cirrhotic liver.

Subunit Structure:

Interacts with MYD88, IRAK1, IRAK4, and TRAF6. Bound to its ligand IL-33, interacts with IL1RAP to form the minimal interleukin-33 signaling complex with a 1:1:1 stoichiometry. Interacts with KIT (bound to KITLG/SCF). A mast cell-specific KITLG/SCF-induced interleukin-33 signaling complex contains IL1RL1, IL1RAP, KIT and MYD88.

Family&Domains:

The TIR domain mediates NAD(+) hydrolase (NADase) activity. Self-association of TIR domains is required for NADase activity.

Belongs to the interleukin-1 receptor family.

References

1). The role of 5-hydroxytryptamine on expulsion of Trichinella spiralis during the intestinal stage. Veterinary parasitology, 2025 (PubMed: 39837238) [IF=2.0]

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