Product: IN35 Antibody
Catalog: DF2534
Description: Rabbit polyclonal antibody to IN35
Application: WB IHC
Reactivity: Human, Rat
Mol.Wt.: 32 kDa; 32kD(Calculated).
Uniprot: P80217
RRID: AB_2839740

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Clonality:
Polyclonal
Specificity:
IN35 Antibody detects endogenous levels of total IN35.
RRID:
AB_2839740
Cite Format: Affinity Biosciences Cat# DF2534, RRID:AB_2839740.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ21753; IFI 35; Ifi-35; Ifi35; IFP 35; IFP35; IN35; IN35_HUMAN; Interferon induced 35 kDa protein; Interferon-induced 35 kDa protein; OTTHUMP00000236626; OTTHUMP00000236627;

Immunogens

Immunogen:

A synthesized peptide derived from human IN35, corresponding to a region within C-terminal amino acids.

Uniprot:
Gene(ID):
Expression:
P80217 IN35_HUMAN:

In a wide range of cell types, including fibroblasts, macrophages, and epithelial cells.

Sequence:
MSAPLDAALHALQEEQARLKMRLWDLQQLRKELGDSPKDKVPFSVPKIPLVFRGHTQQDPEVPKSLVSNLRIHCPLLAGSALITFDDPKVAEQVLQQKEHTINMEECRLRVQVQPLELPMVTTIQMSSQLSGRRVLVTGFPASLRLSEEELLDKLEIFFGKTRNGGGDVDVRELLPGSVMLGFARDGVAQRLCQIGQFTVPLGGQQVPLRVSPYVNGEIQKAEIRSQPVPRSVLVLNIPDILDGPELHDVLEIHFQKPTRGGGEVEALTVVPQGQQGLAVFTSESG

PTMs - P80217 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
S36 Phosphorylation
K40 Ubiquitination
S44 Phosphorylation
K64 Ubiquitination
T138 Phosphorylation
S143 Phosphorylation
K154 Ubiquitination
K161 Ubiquitination
S178 Phosphorylation
K221 Ubiquitination

Research Backgrounds

Function:

Not yet known.

Subcellular Location:

Nucleus.
Note: Nuclear following IFN treatment.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

In a wide range of cell types, including fibroblasts, macrophages, and epithelial cells.

Subunit Structure:

Homodimer. Also interacts with B-ATF.

Family&Domains:

Belongs to the NMI family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.