JAD1B Antibody - #DF2547

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Product: JAD1B Antibody
Catalog: DF2547
Description: Rabbit polyclonal antibody to JAD1B
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 175kDa; 176kD(Calculated).
Uniprot: Q9UGL1
RRID: AB_2839753

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
JAD1B Antibody detects endogenous levels of total JAD1B.
RRID:
AB_2839753
Cite Format: Affinity Biosciences Cat# DF2547, RRID:AB_2839753.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cancer/testis antigen 31; CT31; Histone demethylase JARID1B; JARID1B; Jumonji AT rich interactive domain 1B; Jumonji/ARID domain-containing protein 1B; Kdm5b; KDM5B_HUMAN; Lysine (K) specific demethylase 5B; Lysine demethylase 5B; Lysine specific demethylase 5B; Lysine-specific demethylase 5B; PLU-1; PLU1; PPP1R98; Protein phosphatase 1 regulatory subunit 98; PUT1; putative DNA/chromatin binding motif; RBBP2H1A; RBP2 like; RBP2-H1; Retinoblastoma-binding protein 2 homolog 1; Retinoblastoma-binding protein 2 homolog 1A;

Immunogens

Immunogen:
Uniprot:

Q9UGL1(KDM5B_HUMAN) >>Visit HPA database.

Gene(ID):
KDM5B(10765)
Expression:
Q9UGL1 KDM5B_HUMAN:

Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).

Sequence:
MEAATTLHPGPRPALPLGGPGPLGEFLPPPECPVFEPSWEEFADPFAFIHKIRPIAEQTGICKVRPPPDWQPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKYWELQGSTLKIPHVERKILDLFQLNKLVAEEGGFAVVCKDRKWTKIATKMGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTTDTKDKEYKPHDIPQRQSVQPSETCPPARRAKRMRAEAMNIKIEPEETTEARTHNLRRRMGCPTPKCENEKEMKSSIKQEPIERKDYIVENEKEKPKSRSKKATNAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLIPPLHDVPKGDWRCPKCLAQECSKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRDGKIKLSPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNTLMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHDEMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCVKCKTTCFMSAISCSCKPGLLVCLHHVKELCSCPPYKYKLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKINKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQHSQKLLSEETPSAAELQDLLDVSFEFDVELPQLAEMRIRLEQARWLEEVQQACLDPSSLTLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLKARPRHSLNSLATAVKEIEEIPAYLPNGAALKDSVQRARDWLQDVEGLQAGGRVPVLDTLIELVTRGRSIPVHLNSLPRLETLVAEVQAWKECAVNTFLTENSPYSLLEVLCPRCDIGLLGLKRKQRKLKEPLPNGKKKSTKLESLSDLERALTESKETASAMATLGEARLREMEALQSLRLANEGKLLSPLQDVDIKICLCQKAPAAPMIQCELCRDAFHTSCVAVPSISQGLRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTVNWQHRAQQLLSSGNLKFVQDRVGSGLLYSRWQASAGQVSDTNKVSQPPGTTSFSLPDDWDNRTSYLHSPFSTGRSCIPLHGVSPEVNELLMEAQLLQVSLPEIQELYQTLLAKPSPAQQTDRSSPVRPSSEKNDCCRGKRDGINSLERKLKRRLEREGLSSERWERVKKMRTPKKKKIKLSHPKDMNNFKLERERSYELVRSAETHSLPSDTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTVKDAPSRK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
92
Bovine
92
Sheep
92
Chicken
83
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UGL1 As Substrate

Site PTM Type Enzyme
K63 Ubiquitination
K80 Ubiquitination
T84 Phosphorylation
K100 Ubiquitination
K109 Ubiquitination
Y110 Phosphorylation
K119 Ubiquitination
K126 Ubiquitination
K148 Ubiquitination
T157 Phosphorylation
K158 Ubiquitination
K165 Ubiquitination
K196 Acetylation
K196 Ubiquitination
K206 Ubiquitination
K209 Sumoylation
K209 Ubiquitination
K242 Sumoylation
K242 Ubiquitination
T264 Phosphorylation
K278 Sumoylation
K278 Ubiquitination
K355 Ubiquitination
K363 Ubiquitination
K390 Ubiquitination
K408 Ubiquitination
S415 Phosphorylation
K444 Ubiquitination
S457 Phosphorylation
T476 Phosphorylation
K535 Ubiquitination
K635 Ubiquitination
K639 Ubiquitination
K663 Ubiquitination
K671 Ubiquitination
K729 Ubiquitination
Y730 Phosphorylation
Y734 Phosphorylation
Y736 Phosphorylation
Y742 Phosphorylation
K749 Ubiquitination
K769 Ubiquitination
K774 Ubiquitination
K780 Ubiquitination
K789 Ubiquitination
K790 Ubiquitination
K809 Ubiquitination
S812 Phosphorylation
K821 Ubiquitination
K832 Acetylation
K832 Ubiquitination
K956 Ubiquitination
K974 Ubiquitination
K976 Ubiquitination
K980 Ubiquitination
S986 Phosphorylation
K995 Ubiquitination
K1011 Ubiquitination
S1055 Phosphorylation
K1102 Ubiquitination
K1107 Acetylation
K1121 Sumoylation
K1121 Ubiquitination
S1126 Phosphorylation
S1135 Phosphorylation
K1136 Ubiquitination
T1144 Phosphorylation
S1158 Phosphorylation
K1166 Ubiquitination
S1169 Phosphorylation
K1183 Ubiquitination
K1226 Ubiquitination
S1238 Phosphorylation
K1276 Ubiquitination
S1328 Phosphorylation
S1383 Phosphorylation
S1384 Phosphorylation
K1392 Ubiquitination
S1405 Phosphorylation
S1420 Phosphorylation
K1444 Ubiquitination
K1450 Sumoylation
K1450 Ubiquitination
S1456 Phosphorylation

Research Backgrounds

Function:

Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma. Represses the CLOCK-ARNTL/BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).

Subunit Structure:

Interacts with FOXG1B, PAX9, MYC, MYCN and RB1. Interacts with HDAC1, HDAC4, HDAC5 and HDAC7. Interacts (via PHD-type 1 zinc finger) with histone H3 unmodified at 'Lys-4'; the interaction is inhibited when histone H3 is methylated at 'Arg-2' or 'Lys-4'.

Family&Domains:

Both the JmjC domain and the JmjN domain are required for enzymatic activity. However ARID and PHD-type 1 domain are not required for activity per se but contributed to recognition of the H3(1-21)K4me2 substrate peptide.

The 2 first PHD-type zinc finger domains are required for transcription repression activity.

Belongs to the JARID1 histone demethylase family.

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