JAD1D Antibody | Affinity Biosciences

WB
IHC
Cites

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JAD1D Antibody - Western blot analysis of JAD1D expression in human prostate cancer ,The lane on the left was treated with the antigen-specific peptide.

JAD1D Antibody - Western blot analysis of JAD1D expression in HEK293 cells.

JAD1D Antibody - DF2548 at 1/100 staining Human kidney tissue by IHC-P.

JAD1D Antibody - Figure 1 Comparison of KDM5D expression in tumor cells (A) and benign cells (B) in radical prostatectomy specimens of docetaxel responders and non-responders.

JAD1D Antibody - Fig.

Product:	JAD1D Antibody
Catalog:	DF2548
Description:	Rabbit polyclonal antibody to JAD1D
Application:	WB IHC
Reactivity:	Human
Mol.Wt.:	174kDa; 174kD(Calculated).
Uniprot:	Q9BY66
RRID:	AB_2839754

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

JAD1D Antibody detects endogenous levels of total JAD1D.

RRID:

AB_2839754
Cite Format: Affinity Biosciences Cat# DF2548, RRID:AB_2839754.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

H-Y; Histocompatibility Y antigen; Histone demethylase JARID1D; HY; HYA; JAD1D; Jumonji AT rich interactive domain 1D (RBP2 like); Jumonji/ARID domain-containing protein 1D; KDM5D; KDM5D_HUMAN; KIAA0234; Lysine (K) specific demethylase 5D; Lysine demethylase 5D; Lysine-specific demethylase 5D; Protein SmcY; Selected mouse cDNA on Y human homolog of; SMC homolog Y chromosome; Smcy homolog Y linked;

Immunogens

Immunogen:

Uniprot:

Q9BY66(KDM5D_HUMAN) >>Visit HPA database.

Gene(ID):

KDM5D(8284)

Expression:

Q9BY66 KDM5D_HUMAN:

Expression is highly down-regulated in metastatic prostate tumors.

Sequence:

Q9BY66 KDM5D_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEPGCDEFLPPPECPVFEPSWAEFQDPLGYIAKIRPIAEKSGICKIRPPADWQPPFAVEVDNFRFTPRVQRLNELEAQTRVKLNYLDQIAKFWEIQGSSLKIPNVERKILDLYSLSKIVIEEGGYEAICKDRRWARVAQRLHYPPGKNIGSLLRSHYERIIYPYEMFQSGANHVQCNTHPFDNEVKDKEYKPHSIPLRQSVQPSKFSSYSRRAKRLQPDPEPTEEDIEKHPELKKLQIYGPGPKMMGLGLMAKDKDKTVHKKVTCPPTVTVKDEQSGGGNVSSTLLKQHLSLEPCTKTTMQLRKNHSSAQFIDSYICQVCSRGDEDDKLLFCDGCDDNYHIFCLLPPLPEIPRGIWRCPKCILAECKQPPEAFGFEQATQEYSLQSFGEMADSFKSDYFNMPVHMVPTELVEKEFWRLVSSIEEDVTVEYGADIHSKEFGSGFPVSNSKQNLSPEEKEYATSGWNLNVMPVLDQSVLCHINADISGMKVPWLYVGMVFSAFCWHIEDHWSYSINYLHWGEPKTWYGVPSLAAEHLEEVMKMLTPELFDSQPDLLHQLVTLMNPNTLMSHGVPVVRTNQCAGEFVITFPRAYHSGFNQGYNFAEAVNFCTADWLPAGRQCIEHYRRLRRYCVFSHEELICKMAAFPETLDLNLAVAVHKEMFIMVQEERRLRKALLEKGVTEAEREAFELLPDDERQCIKCKTTCFLSALACYDCPDGLVCLSHINDLCKCSSSRQYLRYRYTLDELPTMLHKLKIRAESFDTWANKVRVALEVEDGRKRSFEELRALESEARERRFPNSELLQRLKNCLSEVEACIAQVLGLVSGQVARMDTPQLTLTELRVLLEQMGSLPCAMHQIGDVKDVLEQVEAYQAEAREALATLPSSPGLLRSLLERGQQLGVEVPEAHQLQQQVEQAQWLDEVKQALAPSAHRGSLVIMQGLLVMGAKIASSPSVDKARAELQELLTIAERWEEKAHFCLEARQKHPPATLEAIIRETENIPVHLPNIQALKEALTKAQAWIADVDEIQNGDHYPCLDDLEGLVAVGRDLPVGLEELRQLELQVLTAHSWREKASKTFLKKNSCYTLLEVLCPCADAGSDSTKRSRWMEKALGLYQCDTELLGLSAQDLRDPGSVIVAFKEGEQKEKEGILQLRRTNSAKPSPLAPSLMASSPTSICVCGQVPAGVGVLQCDLCQDWFHGQCVSVPHLLTSPKPSLTSSPLLAWWEWDTKFLCPLCMRSRRPRLETILALLVALQRLPVRLPEGEALQCLTERAIGWQDRARKALASEDVTALLRQLAELRQQLQAKPRPEEASVYTSATACDPIREGSGNNISKVQGLLENGDSVTSPENMAPGKGSDLELLSSLLPQLTGPVLELPEAIRAPLEELMMEGDLLEVTLDENHSIWQLLQAGQPPDLDRIRTLLELEKFEHQGSRTRSRALERRRRRQKVDQGRNVENLVQQELQSKRARSSGIMSQVGREEEHYQEKADRENMFLTPSTDHSPFLKGNQNSLQHKDSGSSAACPSLMPLLQLSYSDEQQL

PTMs - Q9BY66 As Substrate

Q9BY66

Site	PTM Type	Source
K33	Sumoylation	Uniprot
K33	Ubiquitination	Uniprot
K40	Ubiquitination	Uniprot
K45	Ubiquitination	Uniprot
K82	Ubiquitination	Uniprot
K91	Ubiquitination	Uniprot
K101	Ubiquitination	Uniprot
K130	Ubiquitination	Uniprot
K188	Ubiquitination	Uniprot
K191	Sumoylation	Uniprot
K191	Ubiquitination	Uniprot
K205	Ubiquitination	Uniprot
K235	Ubiquitination	Uniprot
Y239	Phosphorylation	Uniprot
K287	Ubiquitination	Uniprot
K360	Ubiquitination	Uniprot
K413	Ubiquitination	Uniprot
S420	Phosphorylation	Uniprot
K449	Ubiquitination	Uniprot
K672	Ubiquitination	Uniprot
K677	Ubiquitination	Uniprot
Y741	Phosphorylation	Uniprot
K766	Ubiquitination	Uniprot
S884	Phosphorylation	Uniprot
K955	Ubiquitination	Uniprot
K973	Ubiquitination	Uniprot
K983	Ubiquitination	Uniprot
K1010	Ubiquitination	Uniprot
K1074	Acetylation	Uniprot
K1079	Ubiquitination	Uniprot
K1101	Ubiquitination	Uniprot
K1138	Ubiquitination	Uniprot
K1143	Ubiquitination	Uniprot
T1244	Phosphorylation	Uniprot
K1281	Ubiquitination	Uniprot
R1299	Methylation	Uniprot
K1305	Ubiquitination	Uniprot
S1346	Phosphorylation	Uniprot
K1426	Ubiquitination	Uniprot
K1465	Ubiquitination	Uniprot

Research Backgrounds

Q9BY66_KDM5D_HUMAN

Function:

Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May play a role in spermatogenesis. Involved in transcriptional repression of diverse metastasis-associated genes; in this function seems to cooperate with ZMYND8. Suppresses prostate cancer cell invasion. Regulates androgen receptor (AR) transcriptional activity by demethylating H3K4me3 active transcription marks.

Subcellular Location:

Nucleus.

Tissue Specificity:

Expression is highly down-regulated in metastatic prostate tumors.

Subunit Structure:

Interacts with PCGF6, MSH5, ZMYND8, AR.

Family&Domains:

The JmjC domain is required for enzymatic activity.

Belongs to the JARID1 histone demethylase family.

References

1). Curcumin blunts epithelial-mesenchymal transition to alleviate invasion and metastasis of prostate cancer through the JARID1D demethylation. Cancer cell international, 2024 (PubMed: 39218854) [IF=5.8]

Application: WB Species: Mouse Sample: PCa cells

Fig. 1. Knocking down JARID1D can enhance the invasive ability of PCa cells. (A-B) The expression of JARID1D in PCa cell lines (22RV1, LNCaP, PC3, C4-2 and DU145) was analyzed using quantitative RT-PCR (A) and Western blot (B) and quantitative analysis results. (C-D) The expression of JARID1D after 22RV1 cells transfection into lentivirus was analyzed using Western blot and quantitative analysis results. (C) and quantitative RT-PCR (D). (E) Transwell (left) and its quantification (right) analyzed the changes in the invasion ability of the 22RV1 cells after knockdown of JARID1D when compared with the NC group, *P

Application: IHC Species: Mouse Sample:

Fig. 5. Curcumin inhibits PCa lung metastasis caused by JARID1D knockdown through EMT in AR-dependent cells in vivo. (A-B) Bioluminescence image (left) and its quantification (right) of (A) whole mouse and (B) mouse lung tissue after treatment with curcumin. (C) H&E staining results of the mouse lung metastasis tumor tissue after curcumin treatment. (D) The body weight of mice treated with curcumin was monitored every two days. (E) The expression level of JARID1D, AR, MMP2, E-cadherin, N-cadherin, Vimentin in the lung metastasis model induced by the injection of 22RV1-JARID1D cells after treatment with curcumin. EMT, epithelial-mesenchymal transition; PCa, prostate cancer; AR, androgen receptor

2). KDM5D predicts response to docetaxel chemotherapy in metastatic castration resistant prostate cancer patients. Translational Andrology and Urology, 2021 (PubMed: 34804837) [IF=1.9]

Application: IHC Species: Human Sample:

Figure 1 Comparison of KDM5D expression in tumor cells (A) and benign cells (B) in radical prostatectomy specimens of docetaxel responders and non-responders. (C) Absolute number of Ki-67 index (% of positive cells in correlation to total cell number of evaluated area) in docetaxel responders and non-responders. Data presented as mean ± SEM, statistics: unpaired t-test, *, P

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JAD1D Antibody - #DF2548