JAM1 Antibody - #DF2549

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Product: JAM1 Antibody
Catalog: DF2549
Description: Rabbit polyclonal antibody to JAM1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Horse, Sheep, Rabbit, Dog
Mol.Wt.: 33 kDa; 33kD(Calculated).
Uniprot: Q9Y624
RRID: AB_2839755

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(88%), Sheep(88%), Rabbit(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
JAM1 Antibody detects endogenous levels of total JAM1.
RRID:
AB_2839755
Cite Format: Affinity Biosciences Cat# DF2549, RRID:AB_2839755.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD 321; CD321; CD321 antigen; ESTM33; F11 receptor; F11R; JAM 1; JAM A; JAM; JAM-1; JAM-A; JAM1; JAM1_HUMAN; JAMA; JCAM; Jcam1; Junction adhesion molecule 1; Junction adhesion molecule, mouse, homolog of; Junctional adhesion molecule 1; Junctional adhesion molecule A; KAT; Ly106; PAM 1; PAM-1; PAM1; Platelet adhesion molecule 1; Platelet adhesion molecule; Platelet F11 receptor; PRO301; UNQ264;

Immunogens

Immunogen:

A synthesized peptide derived from human JAM1, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9Y624(JAM1_HUMAN) >>Visit HPA database.

Gene(ID):
F11R(50848)
Expression:
Q9Y624 JAM1_HUMAN:

Expressed in endothelium, epithelium and leukocytes (at protein level).

Description:
Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.
Sequence:
MGTKAQVERKLLCLFILAILLCSLALGSVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGVIVAAVLVTLILLGILVFGIWFAYSRGHFDRTKKGTSSKKVIYSQPSARSEGEFKQTSSFLV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
88
Sheep
88
Dog
88
Rabbit
88
Bovine
75
Chicken
75
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration. Involved in platelet activation.

(Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1.

(Microbial infection) Acts as a receptor for Human Rotavirus strain Wa.

PTMs:

N-glycosylated.

Subcellular Location:

Cell junction>Tight junction. Cell membrane>Single-pass type I membrane protein.
Note: Localized at tight junctions of both epithelial and endothelial cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in endothelium, epithelium and leukocytes (at protein level).

Family&Domains:

The Ig-like V-type 2 domain is necessary and sufficient for interaction with integrin alpha-L/beta-2.

Belongs to the immunoglobulin superfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

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