Product: TUBD1 Antibody
Catalog: DF2568
Description: Rabbit polyclonal antibody to TUBD1
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Bovine, Horse
Mol.Wt.: 45 kDa; 51kD(Calculated).
Uniprot: Q9UJT1
RRID: AB_2839774

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(83%), Horse(83%)
Clonality:
Polyclonal
Specificity:
TUBD1 Antibody detects endogenous levels of total TUBD1.
RRID:
AB_2839774
Cite Format: Affinity Biosciences Cat# DF2568, RRID:AB_2839774.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Delta tubulin; Delta-tubulin; FLJ12709; TBD_HUMAN; TUBD; TUBD1; Tubulin delta chain;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
In the elongating spermatid it is associated with the manchette, a specialized microtubule system present during reshaping of the sperm head.
Sequence:
MSIVTVQLGQCGNQIGFEVFDALLSDSHSSQGLCSMRENEAYQASCKERFFSEEENGVPIARAVLVDMEPKVINQMLSKAAQSGQWKYGQHACFCQKQGSGNNWAYGYSVHGPRHEESIMNIIRKEVEKCDSFSGFFIIMSMAGGTGSGLGAFVTQNLEDQYSNSLKMNQIIWPYGTGEVIVQNYNSILTLSHLYRSSDALLLHENDAIHKICAKLMNIKQISFSDINQVLAHQLGSVFQPTYSAESSFHYRRNPLGDLMEHLVPHPEFKMLSVRNIPHMSENSLAYTTFTWAGLLKHLRQMLISNAKMEEGIDRHVWPPLSGLPPLSKMSLNKDLHFNTSIANLVILRGKDVQSADVEGFKDPALYTSWLKPVNAFNVWKTQRAFSKYEKSAVLVSNSQFLVKPLDMIVGKAWNMFASKAYIHQYTKFGIEEEDFLDSFTSLEQVVASYCNL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
83
Bovine
83
Sheep
75
Dog
75
Rabbit
75
Zebrafish
60
Pig
58
Xenopus
50
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UJT1 As Substrate

Site PTM Type Enzyme
K47 Ubiquitination
K71 Ubiquitination
K79 Ubiquitination
K87 Ubiquitination
K97 Ubiquitination
K215 Ubiquitination
K329 Ubiquitination
K362 Ubiquitination
K372 Ubiquitination
K381 Ubiquitination
K388 Ubiquitination
S392 Phosphorylation

Research Backgrounds

Function:

Acts as a positive regulator of hedgehog signaling and regulates ciliary function.

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cell projection>Cilium.
Note: Associated with centrioles. Both cytoplasmic and nuclear. In the elongating spermatid it is associated with the manchette, a specialized microtubule system present during reshaping of the sperm head.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Found in a complex with TEDC1, TEDC2, TUBE1 and TUBD1.

Family&Domains:

Belongs to the tubulin family.

Restrictive clause

 

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