SYCP2 Antibody - #DF2578

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Product: SYCP2 Antibody
Catalog: DF2578
Description: Rabbit polyclonal antibody to SYCP2
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 180kD; 176kD(Calculated).
Uniprot: Q9BX26
RRID: AB_2839784

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(92%), Bovine(92%), Sheep(92%), Rabbit(90%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SYCP2 Antibody detects endogenous levels of total SYCP2.
RRID:
AB_2839784
Cite Format: Affinity Biosciences Cat# DF2578, RRID:AB_2839784.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hsSCP2; SCP 2; SCP-2; Sycp2; SYCP2_HUMAN; Synaptonemal complex lateral element protein; Synaptonemal complex protein 2;

Immunogens

Immunogen:
Uniprot:

Q9BX26(SYCP2_HUMAN) >>Visit HPA database.

Gene(ID):
SYCP2(10388)
Description:
Major component of the axial/lateral elements of synaptonemal complexes (SCS) during meiotic prophase. May be involved in the organization of chromatin by temporarily binding to DNA scaffold attachment regions. Requires SYCP3, but not SYCP1, in order to be incorporated into the axial/lateral elements.
Sequence:
MPIRPDLQQLEKCIDDALRKNDFKPLKTLLQIDICEDVKIKCSKQFFHKVDNLICRELNKEDIHNVSAILVSVGRCGKNISVLGQAGLLTMIKQGLIQKMVAWFEKSKDIIQSQGNSKDEAVLNMIEDLVDLLLVIHDVSDEGKKQVVESFVPRICSLVIDSRVNICIQQEIIKKMNAMLDKMPQDARKILSNQEMLILMSSMGERILDAGDYDLQVGIVEALCRMTTEKQRQELAHQWFSMDFIAKAFKRIKDSEFETDCRIFLNLVNGMLGDKRRVFTFPCLSAFLDKYELQIPSDEKLEEFWIDFNLGSQTLSFYIAGDNDDHQWEAVTVPEEKVQIYSIEVRESKKLLTIILKNTVKISKREGKELLLYFDASLEITNVTQKIFGATKHRESIRKQGISVAKTSLHILFDASGSQILVPESQISPVGEELVSLKEKSKSPKEFAKPSKYIKNSDKGNRNNSQLEKTTPSKRKMSEASMIVSGADRYTMRSPVLFSNTSIPPRRRRIKPPLQMTSSAEKPSVSQTSENRVDNAASLKSRSSEGRHRRDNIDKHIKTAKCVENTENKNVEFPNQNFSELQDVIPDSQAAEKRDHTILPGVLDNICGNKIHSKWACWTPVTNIELCNNQRASTSSGDTLNQDIVINKKLTKQKSSSSISDHNSEGTGKVKYKKEQTDHIKIDKAEVEVCKKHNQQQNHPKYSGQKNTENAKQSDWPVESETTFKSVLLNKTIEESLIYRKKYILSKDVNTATCDKNPSASKNVQSHRKAEKELTSELNSWDSKQKKMREKSKGKEFTNVAESLISQINKRYKTKDDIKSTRKLKESLINSGFSNKPVVQLSKEKVQKKSYRKLKTTFVNVTSECPVNDVYNFNLNGADDPIIKLGIQEFQATAKEACADRSIRLVGPRNHDELKSSVKTKDKKIITNHQKKNLFSDTETEYRCDDSKTDISWLREPKSKPQLIDYSRNKNVKNHKSGKSRSSLEKGQPSSKMTPSKNITKKMDKTIPEGRIRLPRKATKTKKNYKDLSNSESECEQEFSHSFKENIPVKEENIHSRMKTVKLPKKQQKVFCAETEKELSKQWKNSSLLKDAIRDNCLDLSPRSLSGSPSSIEVTRCIEKITEKDFTQDYDCITKSISPYPKTSSLESLNSNSGVGGTIKSPKNNEKNFLCASESCSPIPRPLFLPRHTPTKSNTIVNRKKISSLVLTQETQNSNSYSDVSSYSSEERFMEIESPHINENYIQSKREESHLASSLSKSSEGREKTWFDMPCDATHVSGPTQHLSRKRIYIEDNLSNSNEVEMEEKGERRANLLPKKLCKIEDADHHIHKMSESVSSLSTNDFSIPWETWQNEFAGIEMTYETYERLNSEFKRRNNIRHKMLSYFTTQSWKTAQQHLRTMNHQSQDSRIKKLDKFQFIIIEELENFEKDSQSLKDLEKEFVDFWEKIFQKFSAYQKSEQQRLHLLKTSLAKSVFCNTDSEETVFTSEMCLMKEDMKVLQDRLLKDMLEEELLNVRRELMSVFMSHERNANV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Pig
92
Bovine
92
Sheep
92
Rabbit
90
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BX26 As Substrate

Site PTM Type Enzyme
K357 Acetylation
K361 Acetylation
S436 Phosphorylation
T470 Phosphorylation
S478 Phosphorylation
S481 Phosphorylation
S485 Phosphorylation
T501 Phosphorylation
S502 Phosphorylation
S543 Phosphorylation
S544 Phosphorylation
K681 Ubiquitination
Y966 Phosphorylation
S967 Phosphorylation
K992 Acetylation
T1019 Phosphorylation
T1021 Phosphorylation
S1040 Phosphorylation
S1042 Phosphorylation
S1056 Phosphorylation
T1060 Phosphorylation
K1065 Acetylation
K1066 Acetylation
S1110 Phosphorylation
S1111 Phosphorylation
Y1130 Phosphorylation
S1138 Phosphorylation
Y1140 Phosphorylation
Y1241 Phosphorylation
S1249 Phosphorylation
S1253 Phosphorylation
S1254 Phosphorylation
S1256 Phosphorylation
S1523 Phosphorylation

Research Backgrounds

Function:

Major component of the axial/lateral elements of synaptonemal complexes (SCS) during meiotic prophase. Plays a role in the assembly of synaptonemal complexes. Required for normal meiotic chromosome synapsis during oocyte and spermatocyte development and for normal male and female fertility. Required for insertion of SYCP3 into synaptonemal complexes. May be involved in the organization of chromatin by temporarily binding to DNA scaffold attachment regions. Requires SYCP3, but not SYCP1, in order to be incorporated into the axial/lateral elements.

PTMs:

Phosphorylated.

Subcellular Location:

Nucleus. Chromosome.
Note: In axial/lateral elements of the tripartite segments of synaptonemal complexes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the lateral elements of synaptonemal complexes. Heterodimer with SYCP3 (By similarity). Interacts with SMC1A and SMC3 (By similarity). Interacts with TEX11 (By similarity).

Family&Domains:

Belongs to the SYCP2 family.

References

1). Characterization of sheep spermatogenesis through single‐cell RNA sequencing. FASEB JOURNAL, 2021 (PubMed: 33197070) [IF=4.4]

Application: IHC    Species: sheep    Sample: testicular cells

FIGURE 7 Expression level and distribution of several potential genes. A, The expression distribution of EZH2, SOX18, SYCP2, PCNA, and PRKCD in sheep testicular cells. B, Immunohistochemistry analysis the expression location of EZH2, SOX18, SYCP2, PCNA, and PRKCD in sheep testis. The testis morphology was observed under 400× magnification by a microscope

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