ALKBH4 Antibody - #DF2584
| Product: | ALKBH4 Antibody |
| Catalog: | DF2584 |
| Description: | Rabbit polyclonal antibody to ALKBH4 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| Mol.Wt.: | 36 kDa; 34kD(Calculated). |
| Uniprot: | Q9NXW9 |
| RRID: | AB_2839790 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF2584, RRID:AB_2839790.
Fold/Unfold
2010004B12Rik; ABH4; Abj4; AlkB, alkylation repair homolog 4 (E. coli); Alkylated DNA repair protein alkB homolog 4; FLJ20013; Probable alpha-ketoglutarate dependent dioxygenase ABH4; RGD1308608;
Immunogens
- Q9NXW9 ALKB4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAAAETPEVLRECGCKGIRTCLICERQRGSDPPWELPPAKTYRFIYCSDTGWAVGTEESDFEGWAFPFPGVMLIEDFVTREEEAELVRLMDRDPWKLSQSGRRKQDYGPKVNFRKQKLKTEGFCGLPSFSREVVRRMGLYPGLEGFRPVEQCNLDYCPERGSAIDPHLDDAWLWGERLVSLNLLSPTVLSMCREAPGSLLLCSAPSAAPEALVDSVIAPSRSVLCQEVEVAIPLPARSLLVLTGAARHQWKHAIHRRHIEARRVCVTFRELSAEFGPGGRQQELGQELLRIALSFQGRPV
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NXW9 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| Y109 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| Y158 | Phosphorylation | Uniprot | |
| R249 | Methylation | Uniprot |
Research Backgrounds
Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes. Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. In addition to proteins, also demethylates DNA: specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA, thereby regulating Polycomb silencing (By similarity).
Cytoplasm. Nucleus. Nucleus>Nucleolus. Midbody.
Note: Associates with the contractile ring and midbody.
Widely expressed, with highest expression in pancreas, ovary and spleen.
Interacts with ZFHX3, MLLT3, MLLT1, HSF4, EP300, TES, EIF3C, MTMR6 and PSMA6.
Belongs to the alkB family.
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