ALKBH5 Antibody - #DF2585

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Product: ALKBH5 Antibody
Catalog: DF2585
Description: Rabbit polyclonal antibody to ALKBH5
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 44 kDa, 37 kDa; 44kD(Calculated).
Uniprot: Q6P6C2
RRID: AB_2839791

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 100ul $280 In stock
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Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
ALKBH5 Antibody detects endogenous levels of total ALKBH5.
RRID:
AB_2839791
Cite Format: Affinity Biosciences Cat# DF2585, RRID:AB_2839791.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ABH5; AlkB, alkylation repair homolog 5 (E. coli); AlkB, alkylation repair homolog 5; Alkylated DNA repair protein alkB homolog 5; Alpha ketoglutarate dependent dioxygenase alkB homolog 5; OFOXD; OFOXD1; Oxoglutarate and iron-dependent oxygenase domain containing; Probable alpha ketoglutarate dependent dioxygenase ABH5; RNA demethylase ALKBH5;

Immunogens

Immunogen:
Uniprot:

Q6P6C2(ALKB5_HUMAN) >>Visit HPA database.

Gene(ID):
ALKBH5(54890)
Expression:
Q6P6C2 ALKB5_HUMAN:

Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.

Sequence:
MAAASGYTDLREKLKSMTSRDNYKAGSREAAAAAAAAVAAAAAAAAAAEPYPVSGAKRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGDVDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRKTRLDAPRLETKSLSSSVLPPSYASDRLSGNNRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCSEAAGSPARKVKMRRH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
92
Chicken
82
Xenopus
63
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6P6C2 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S5 Phosphorylation
K15 Ubiquitination
Y51 Phosphorylation
S54 Phosphorylation
K57 Acetylation
K57 Ubiquitination
Y60 Phosphorylation
S64 Phosphorylation
S69 Phosphorylation
Y71 Phosphorylation
K86 Acetylation
S96 Phosphorylation
K102 Sumoylation
K102 Ubiquitination
S112 Phosphorylation
K116 Ubiquitination
K132 Acetylation
K132 Ubiquitination
K147 Ubiquitination
K235 Acetylation
K235 Ubiquitination
R250 Methylation
S252 Phosphorylation
T294 Phosphorylation
K295 Ubiquitination
S296 Phosphorylation
S312 Phosphorylation
K321 Acetylation
K321 Ubiquitination
K328 Sumoylation
S350 Phosphorylation
R359 Methylation
S361 Phosphorylation
S363 Phosphorylation
S371 Phosphorylation
Y372 Phosphorylation
S374 Phosphorylation
S375 Phosphorylation
S379 Phosphorylation
S384 Phosphorylation

Research Backgrounds

Function:

Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells (By similarity).

Subcellular Location:

Nucleus speckle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the alkB family.

References

1). Pseudorabies virus exploits N6-methyladenosine modification to promote viral replication. Frontiers in Microbiology, 2023 (PubMed: 36819040) [IF=5.2]
2). Comprehensive Analysis of the Transcriptome-Wide m6A Methylation Modification Difference in Liver Fibrosis Mice by High-Throughput m6A Sequencing. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 34869362) [IF=4.6]

Application: WB    Species: Mice    Sample: Liver Fibrosis

FIGURE 7 Verification of m6A methylation level and methylase expression in LF. (A) The m6A methylation level in LF. (B) Semiquantitative analysis of m6A methylation. (C) IGV plots of the WTAP m6A level. (D) IGV plots of the ALKBH5 m6A level. (E) IGV plots of the YTHDF1 m6A level. (F) IGV plots of the WTAP expression level. (G) IGV plots of the ALKBH5 expression level. (H) IGV plots of the YTHDF1 expression level. (I) mRNA expression level of WTAP. (J) mRNA expression level of ALKBH5. (K) mRNA expression level of YTHDF1. (L) Protein expression levels of WTAP. (M) Protein expression levels of ALKBH5. (N) Protein expression levels of YTHDF1. (O) Semiquantitative analysis of WTAP protein. (P) Semiquantitative analysis of YTHDF1 protein. (Q) Semiquantitative analysis of ALKBH5 protein. ## p < 0.01 compared with the control group, # p < 0.05 compared with the control group.
3). Hypoxia triggers cardiomyocyte apoptosis via regulating the m6A methylation-mediated LncMIAT/miR-708-5p/p53 axis. Heliyon, 2024 (PubMed: 38961902) [IF=4.0]

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