NFE2 Antibody - #DF2595
| Product: | NFE2 Antibody |
| Catalog: | DF2595 |
| Description: | Rabbit polyclonal antibody to NFE2 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 41 kDa; 41kD(Calculated). |
| Uniprot: | Q16621 |
| RRID: | AB_2839801 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF2595, RRID:AB_2839801.
Fold/Unfold
erythroid-derived 2 45 kDa subunit; Leucine zipper protein NF-E2; Leucine zipper protein NFE2; NF E2; nfe2; NFE2_HUMAN; Nuclear factor (erythroid derived 2), 45kDa; nuclear factor (erythroid-derived 2), 45kD; Nuclear factor; nuclear factor, erythroid 2; Nuclear factor, erythroid derived 2 45 kDa subunit; p45; p45 NF-E2; p45 NFE2; Transcription factor NF-E2 45 kDa subunit; Transcription factor NFE2 45 kDa subunit;
Immunogens
- Q16621 NFE2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSPCPPQQSRNRVIQLSTSELGEMELTWQEIMSITELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSIHPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQEDPESDSGLSLNYSDAESLELEGTEAGRRRSEYVEMYPVEYPYSLMPNSLAHSNYTLPAAETPLALEPSSGPVRAKPTARGEAGSRDERRALAMKIPFPTDKIVNLPVDDFNELLARYPLTESQLALVRDIRRRGKNKVAAQNCRKRKLETIVQLERELERLTNERERLLRARGEADRTLEVMRQQLTELYRDIFQHLRDESGNSYSPEEYALQQAADGTIFLVPRGTKMEATD
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q16621 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| S157 | Phosphorylation | P45983 (MAPK8) | Uniprot |
| Y176 | Phosphorylation | Uniprot | |
| Y180 | Phosphorylation | Uniprot | |
| Y182 | Phosphorylation | Uniprot | |
| Y194 | Phosphorylation | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| K368 | Sumoylation | Uniprot | |
| K368 | Ubiquitination | Uniprot |
Research Backgrounds
Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
Phosphorylated on serine residues. In undifferentiated erythrocytes, phosphorylated by MAPK8 which then leads to ubiquitination and protein degradation.
Sumoylated. Sumoylation is required for translocation to nuclear bodies PODs, anchoring to the gene loci, and transactivation of the beta-globin gene.
Ubiquitinated mainly by 'Lys63'-linked ubiquitin. Polyubiquitination with 'Lys63'-linked ubiquitin by ITCH retains NFE2 in the cytoplasm preventing its transactivation activity. In undifferentiated erythrocyte, ubiquitinated after MAPK8-mediatd phosphorylation leading to protein degradation (By similarity).
Nucleus>PML body. Cytoplasm.
Note: The sumoylated form locates to the nuclear bodies PML oncogenic domains (PODs). Translocated to the cytoplasm through interaction with ITCH.
Expressed in hematopoietic cells and also in colon and testis.
Homodimer; can bind DNA as a homodimer. Erythroid transcription activator nuclear factor erythroid-derived 2 (NF-E2), composed of a heterodimer of NFE2 and MAFK, possesses transactivation activity on beta-globin. Also forms high affinity heterodimer with MAFG; the interaction promotes erythropoiesis. Interacts (via the PXY motif 1) with ITCH (via the WW 1 domain); the interaction promotes 'Lys63'-linked ubiquitination of NFE2, translocates it to the cytoplasm and inhibits its transactivation activity. Interacts with KMT2D/MLL2; the interaction promotes transactivation of the beta-globin locus (By similarity). Interacts with MAPK8 (phosphorylated form); the interaction leads to phosphorylation of NFE2 in undifferentiated cells (By similarity).
The PXY motifs are required for binding WW domains. PXY1 is required to promote transactivation of beta-globin and for hyperacetylation of histone H3, but not for binding to the HS2 promoter site (By similarity).
Belongs to the bZIP family. CNC subfamily.
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