NDFIP2 Antibody - #DF2596
| Product: | NDFIP2 Antibody |
| Catalog: | DF2596 |
| Description: | Rabbit polyclonal antibody to NDFIP2 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Rabbit, Dog |
| Mol.Wt.: | 36 kDa; 36kD(Calculated). |
| Uniprot: | Q9NV92 |
| RRID: | AB_2839802 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF2596, RRID:AB_2839802.
Fold/Unfold
Ndfip2; NEDD4 family interacting protein 2; NEDD4 family-interacting protein 2; NEDD4 WW domain binding protein 5A; NEDD4 WW domain-binding protein 5A; NFIP2_HUMAN; Putative MAPK activating protein PM04/PM05/PM06/PM07; Putative MAPK-activating protein PM04/PM05/PM06/PM07; Putative NF kappa B activating protein 413; Putative NF-kappa-B-activating protein 413;
Immunogens
- Q9NV92 NFIP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARRRSQRVCASGPSMLNSARGAPELLRGTATNAEVSAAAAGATGSEELPPGDRGCRNGGGRGPAATTSSTGVAVGAEHGEDSLSRKPDPEPGRMDHHQPGTGRYQVLLNEEDNSESSAIEQPPTSNPAPQIVQAASSAPALETDSSPPPYSSITVEVPTTSDTEVYGEFYPVPPPYSVATSLPTYDEAEKAKAAAMAAAAAETSQRIQEEECPPRDDFSDADQLRVGNDGIFMLAFFMAFIFNWLGFCLSFCITNTIAGRYGAICGFGLSLIKWILIVRFSDYFTGYFNGQYWLWWIFLVLGLLLFFRGFVNYLKVRNMSESMAAAHRTRYFFLL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NV92 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Uniprot | |
| Y151 | Phosphorylation | Uniprot | |
| Y167 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y171 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y177 | Phosphorylation | Uniprot | |
| Y186 | Phosphorylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| T204 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| Y262 | Phosphorylation | Uniprot | |
| S271 | Phosphorylation | Uniprot |
Research Backgrounds
Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. Negatively regulates KCNH2 potassium channel activity by decreasing its cell-surface expression and interfering with channel maturation through recruitment of NEDD4L to the Golgi apparatus and multivesicular body where it mediates KCNH2 degradation. May modulate EGFR signaling. Together with NDFIP1, limits the cytokine signaling and expansion of effector Th2 T-cells by promoting degradation of JAK1, probably by ITCH- and NEDD4L-mediated ubiquitination (By similarity).
Ubiquitinated by NEDD4 and ITCH. Also ubiquitinated by NEDD4L. Ubiquitination by NEDD4 or NEDD4L does not affect turnover (By similarity).
Undergoes transient tyrosine-phosphorylation following EGF stimulation, most probably catalyzed by SRC. Phosphorylation on Tyr-151, Tyr-171 and Tyr-177 are dependent on the phosphorylation on Tyr-167. Also phosphorylated by LYN and FYN.
Endosome membrane>Multi-pass membrane protein. Golgi apparatus membrane. Endosome>Multivesicular body membrane.
Expressed in brain, lung, heart, skeletal muscle, kidney, liver and placenta.
Forms heterodimers with NDFIP1. Interacts with HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4. Interacts with NEDD4L. Interacts with PTEN. When phosphorylated at Tyr-167, interacts with SRC and LYN SH2 domain. May thus act as a scaffold that recruits SRC to NDFIP1, enhancing NDFIP1 phosphorylation. Interacts with SLC11A2/DMT1. May interact with phosphorylated EGFR. Interacts with KCNH2.
The PPxY motifs are required for E3 ubiquitin-protein ligase activation and for ubiquitination.
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