ING5 Antibody - #DF2634

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Product: ING5 Antibody
Catalog: DF2634
Description: Rabbit polyclonal antibody to ING5
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit, Dog
Mol.Wt.: 28 kDa; 28kD(Calculated).
Uniprot: Q8WYH8
RRID: AB_2839840

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(82%), Rabbit(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
ING5 Antibody detects endogenous levels of total ING5.
RRID:
AB_2839840
Cite Format: Affinity Biosciences Cat# DF2634, RRID:AB_2839840.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1700001C14Rik; 1700027H23Rik; 1810018M11Rik; FLJ23842; ING 5; Ing5; ING5_HUMAN; Inhibitor of growth family member 5; Inhibitor of growth protein 5; p28 ING 5; p28 ING5; p28ING5;

Immunogens

Immunogen:
Uniprot:

Q8WYH8(ING5_HUMAN) >>Visit HPA database.

Gene(ID):
ING5(84289)
Expression:
Q8WYH8 ING5_HUMAN:

Down-regulated in bone marrow cells in acute myeloid leukemia patients as compared with normal bone marrow cells.

Description:
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator.
Sequence:
MATAMYLEHYLDSIENLPCELQRNFQLMRELDQRTEDKKAEIDILAAEYISTVKTLSPDQRVERLQKIQNAYSKCKEYSDDKVQLAMQTYEMVDKHIRRLDADLARFEADLKDKMEGSDFESSGGRGLKKGRGQKEKRGSRGRGRRTSEEDTPKKKKHKGGSEFTDTILSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGCDNPDCPIEWFHFACVDLTTKPKGKWFCPRCVQEKRKKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
92
Rabbit
92
Horse
82
Chicken
73
Xenopus
67
Bovine
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WYH8 As Substrate

Site PTM Type Enzyme
K54 Ubiquitination
S57 Phosphorylation
K67 Ubiquitination
K95 Ubiquitination
R106 Methylation
K112 Acetylation
K114 Acetylation
S118 Phosphorylation
S122 Phosphorylation
R126 Methylation
K129 Acetylation
K130 Acetylation
K135 Acetylation
T147 Phosphorylation
S148 Phosphorylation
T152 Phosphorylation P24941 (CDK2)
K154 Acetylation
K155 Acetylation
K222 Acetylation
K226 Acetylation

Research Backgrounds

Function:

Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. Inhibits cell growth, induces a delay in S-phase progression and enhances Fas-induced apoptosis in an INCA1-dependent manner.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Down-regulated in bone marrow cells in acute myeloid leukemia patients as compared with normal bone marrow cells.

Subunit Structure:

Interacts with H3K 4me3 and to a lesser extent with H3K4me2. Component of the HBO1 complex composed at least of ING4 or ING5, KAT7/HBO1, MEAF6, and one of JADE1, JADE2 and JADE3. Component of the MOZ/MORF complex composed at least of ING5, KAT6A, KAT6B, MEAF6 and one of BRPF1, BRD1/BRPF2 and BRPF3. Interacts with EP300 and TP53. Interacts with INCA1.

Family&Domains:

The PHD-type zinc finger mediates the binding to H3K4me3.

Belongs to the ING family.

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