CIRBP Antibody - #DF2643

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Product: CIRBP Antibody
Catalog: DF2643
Description: Rabbit polyclonal antibody to CIRBP
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 18 kDa; 19kD(Calculated).
Uniprot: Q14011
RRID: AB_2839849

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(86%), Sheep(86%), Dog(86%), Chicken(86%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
CIRBP Antibody detects endogenous levels of total CIRBP.
RRID:
AB_2839849
Cite Format: Affinity Biosciences Cat# DF2643, RRID:AB_2839849.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A18 hnRNP; A18HNRNP; cirbp; CIRBP_HUMAN; CIRP; Cold inducible RNA binding protein; Cold-inducible RNA-binding protein; Glycine rich RNA binding protein; Glycine-rich RNA-binding protein CIRP; testicular tissue protein Li 39;

Immunogens

Immunogen:
Uniprot:

Q14011(CIRBP_HUMAN) >>Visit HPA database.

Gene(ID):
CIRBP(1153)
Expression:
Q14011 CIRBP_HUMAN:

Ubiquitous.

Description:
Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.
Sequence:
MASDEGKLFVGGLSFDTNEQSLEQVFSKYGQISEVVVVKDRETQRSRGFGFVTFENIDDAKDAMMAMNGKSVDGRQIRVDQAGKSSDNRSRGYRGGSAGGRGFFRGGRGRGRGFSRGGGDRGYGGNRFESRSGGYGGSRDYYSSRSQSGGYSDRSSGGSYRDSYDSYATHNE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Xenopus
100
Bovine
86
Sheep
86
Dog
86
Chicken
86
Pig
0
Horse
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14011 As Substrate

Site PTM Type Enzyme
S21 Phosphorylation
S33 Phosphorylation
T43 Phosphorylation
R47 Methylation
K70 Acetylation
S71 Phosphorylation
K84 Ubiquitination
R94 Methylation
R101 Methylation
R105 Methylation
R116 Methylation
R121 Methylation
Y123 Phosphorylation
R127 Methylation
S130 Phosphorylation
R131 Methylation
S132 Phosphorylation
Y135 Phosphorylation
S138 Phosphorylation
R139 Methylation
Y141 Phosphorylation
Y142 Phosphorylation
S143 Phosphorylation
S144 Phosphorylation
R145 Methylation
S146 Phosphorylation
S148 Phosphorylation
Y151 Phosphorylation
S152 Phosphorylation
R154 Methylation
S155 Phosphorylation
S156 Phosphorylation
S159 Phosphorylation
Y160 Phosphorylation
S163 Phosphorylation
Y164 Phosphorylation
S166 Phosphorylation
Y167 Phosphorylation
T169 Phosphorylation

Research Backgrounds

Function:

Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.

PTMs:

Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs (By similarity).

Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation.

Subcellular Location:

Nucleus>Nucleoplasm. Cytoplasm.
Note: Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with EIF4G1. Associates with ribosomes.

Family&Domains:

Both the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region. Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression (By similarity).

References

1). mTOR Modulates the Endoplasmic Reticulum Stress-Induced CD4+ T Cell Apoptosis Mediated by ROS in Septic Immunosuppression. MEDIATORS OF INFLAMMATION, 2022 (PubMed: 35915740) [IF=4.4]

Application: WB    Species: Mouse    Sample:

Figure 6 CD4+ T cells were assessed for the expression of mTOR pathway-associated proteins, ERS-associated proteins, and apoptosis-associated proteins in WT, LCK-mTOR, LCK-TSC1, CLP, CLP+CIRP-Ab, CLP+NAC, LCK-TSC1+CLP, and Lck-mTOR+CLP mouse groups. Data are mean ± SD. Number of mice per group = 4 (one-way ANOVA Tukey's post hoc test). CD4+ T cells were purified from CIRP-Ab-treated mice, NAC-treated mice, TSC1 knockout mice, and mTOR mouse spleen lymphocytes. Total proteins were western blotted to identify the expression patterns of mTOR pathway proteins (P-mTOR, mTOR, p70s6k, p-p70s6k, P-4EBP, and 4EBP) (a); CIRP and ERS-associated proteins (GRP78 and CHOP) (b); and apoptosis-related proteins (caspase-3, Bax, and Bcl-2) (c).
2). The protective effect of C23 in a rat model of cardiac arrest and resuscitation. SHOCK, 2023 (PubMed: 36930651) [IF=2.7]

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