RASEF Antibody - #DF2646
(1)
| Product: | RASEF Antibody |
| Catalog: | DF2646 |
| Description: | Rabbit polyclonal antibody to RASEF |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Dog |
| Mol.Wt.: | 85-100 kDa; 83kD(Calculated). |
| Uniprot: | Q8IZ41 |
| RRID: | AB_2839852 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Pig(82%), Bovine(91%), Horse(82%), Sheep(91%), Dog(91%)
Clonality:
Polyclonal
Specificity:
RASEF Antibody detects endogenous levels of total RASEF.
RRID:
AB_2839852
Cite Format: Affinity Biosciences Cat# DF2646, RRID:AB_2839852.
Cite Format: Affinity Biosciences Cat# DF2646, RRID:AB_2839852.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
FLJ31614; RAB45; RAB45 member RAS oncogene family; RAS and EF hand domain containing; RAS and EF hand domain containing protein; Ras related protein Rab 45;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q8IZ41 RASEF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEADGDGEELARLRSVFAACDANRSGRLEREEFRALCTELRVRPADAEAVFQRLDADRDGAITFQEFARGFLGSLRGGRRRDWGPLDPAPAVSEAGPETHDSEEDEGDEDAAAALATSCGPASPGRAWQDFQARLGDEAKFIPREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALSDLRRQYETEVGDLQVTIKKLRKLEEQSKRVSQKEDVAALKKQIYDLSMENQKVKKDLLEAQTNIAFLQSELDALKSDYADQSLNTERDLEIIRAYTEDRNSLERQIEILQTANRKLHDSNDGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFIGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCFDSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDVPDIRDEETFGLEDVASVLDWKPQGSVSEGSIVSSSRKPISALSPQTDLVDDNAKSFSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Bovine
91
Sheep
91
Dog
91
Pig
82
Horse
82
Rabbit
73
Chicken
64
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8IZ41 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S74 | Phosphorylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| Y239 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| Y277 | Phosphorylation | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| K366 | Ubiquitination | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| Y399 | Phosphorylation | Uniprot | |
| S414 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| S523 | Phosphorylation | Uniprot | |
| T526 | Phosphorylation | Uniprot | |
| S535 | Phosphorylation | Uniprot | |
| T573 | Phosphorylation | Uniprot | |
| T597 | Phosphorylation | Uniprot | |
| S717 | Phosphorylation | Uniprot | |
| S719 | Phosphorylation | Uniprot | |
| T721 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Binds predominantly GDP, and also GTP.
Subcellular Location:
Cytoplasm>Perinuclear region.
Tissue Specificity:
Down-regulated in cutaneous melanoma cells but not in breast cancer cells.
Subunit Structure:
Homodimer.
Family&Domains:
Belongs to the small GTPase superfamily. Rab family.
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