Product: XRCC5 Antibody
Catalog: BF0183
Description: Mouse monoclonal antibody to XRCC5
Reactivity: Human, Mouse, Monkey
Mol.Wt.: 86kDa; 83kD(Calculated).
Uniprot: P13010
RRID: AB_2833930

View similar products>>

   Size Price Inventory
 50ul $250 In stock
 100ul $350 In stock
 200ul $450 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000, IF/ICC 1:200-1:1000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Monoclonal [AFB1907]
XRCC5 antibody detects endogenous levels of total XRCC5.
Cite Format: Affinity Biosciences Cat# BF0183, RRID:AB_2833930.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


86 kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 2; ATP dependent DNA helicase II 80 kDa subunit; ATP dependent DNA helicase II 86 Kd subunit; ATP dependent DNA helicase II; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box binding factor 85 kDa; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; KARP 1; KARP1; Ku 80; Ku autoantigen 80kDa; Ku80; Ku86; Ku86 autoantigen related protein 1; KUB 2; KUB2; Lupus Ku autoantigen protein p86; NFIV; Nuclear factor IV; Thyroid lupus autoantigen; Thyroid-lupus autoantigen; TLAA; X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining); X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); X-ray repair cross-complementing protein 5; Xray repair complementing defective repair in Chinese hamster cells 5; XRCC 5; XRCC5; XRCC5_HUMAN;



Purified recombinant fragment of human XRCC5 expressed in E. Coli.

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

PTMs - P13010 As Substrate

Site PTM Type Enzyme
K36 Ubiquitination
T39 Phosphorylation
K51 Sumoylation
S102 Phosphorylation
K125 Ubiquitination
R141 Methylation
K144 Acetylation
K144 Ubiquitination
S145 Phosphorylation
K155 Acetylation
K155 Ubiquitination
K156 Ubiquitination
K171 Ubiquitination
S175 Phosphorylation
S191 Phosphorylation
K195 Acetylation
K195 Sumoylation
K195 Ubiquitination
T198 Phosphorylation
K202 Ubiquitination
Y225 Phosphorylation
K233 Ubiquitination
C235 S-Nitrosylation
K238 Ubiquitination
S244 Phosphorylation
C249 S-Nitrosylation
S255 Phosphorylation
S258 Phosphorylation
K265 Acetylation
K265 Ubiquitination
K274 Ubiquitination
T277 Phosphorylation
K282 Ubiquitination
K285 Sumoylation
K285 Ubiquitination
K291 Ubiquitination
Y295 Phosphorylation
C296 S-Nitrosylation
K307 Sumoylation
K307 Ubiquitination
S318 Phosphorylation
S324 Phosphorylation
K325 Sumoylation
K325 Ubiquitination
K332 Acetylation
K332 Methylation
K332 Sumoylation
K332 Ubiquitination
K334 Methylation
K334 Ubiquitination
S335 Phosphorylation
K338 Acetylation
K338 Sumoylation
K338 Ubiquitination
C339 S-Nitrosylation
S341 Phosphorylation
C346 S-Nitrosylation
K347 Ubiquitination
K363 Acetylation
K363 Ubiquitination
S378 Phosphorylation
S379 Phosphorylation
K399 Acetylation
K399 Ubiquitination
Y416 Phosphorylation
S436 Phosphorylation
S437 Phosphorylation
K439 Ubiquitination
K443 Acetylation
K443 Sumoylation
K443 Ubiquitination
K465 Ubiquitination
K466 Acetylation
K466 Ubiquitination
K469 Ubiquitination
T472 Phosphorylation
K481 Ubiquitination
C493 S-Nitrosylation
T523 Phosphorylation
T524 Phosphorylation
K525 Ubiquitination
S531 Phosphorylation
K532 Acetylation
K532 Sumoylation
K532 Ubiquitination
K534 Acetylation
K534 Sumoylation
K534 Ubiquitination
T535 Phosphorylation
K543 Acetylation
K543 Sumoylation
K543 Ubiquitination
K544 Ubiquitination
K545 Ubiquitination
K565 Acetylation
K565 Sumoylation
K565 Ubiquitination
K566 Acetylation
K566 Ubiquitination
K568 Sumoylation
T569 Phosphorylation
S577 Phosphorylation P78527 (PRKDC)
S579 Phosphorylation
S580 Phosphorylation P78527 (PRKDC)
S585 Phosphorylation
T629 Phosphorylation
R640 Methylation
K648 Acetylation
K648 Ubiquitination
K660 Acetylation
K660 Methylation
K660 Sumoylation
K660 Ubiquitination
K665 Acetylation
K665 Ubiquitination
S692 Phosphorylation
S695 Phosphorylation
K702 Acetylation
K702 Methylation
K702 Ubiquitination
K710 Ubiquitination
S712 Phosphorylation
T715 Phosphorylation P78527 (PRKDC)

Research Backgrounds


Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.


ADP-ribosylated by PARP3.

Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.


Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination following DNA damage, leading to its degradation and removal from DNA damage sites. Ubiquitinated by RNF138, leading to remove the Ku complex from DNA breaks.

Subcellular Location:

Nucleus. Nucleus>Nucleolus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. Additional components of the NHEJ complex include NHEJ1/XLF and PAXX. The dimer also associates with NAA15, and this complex displays DNA binding activity towards the osteocalcin FGF response element (OCFRE). In addition, XRCC5 binds to the osteoblast-specific transcription factors MSX2 and RUNX2. Interacts with ELF3. May interact with APLF. The XRCC5/XRCC6 dimer associates in a DNA-dependent manner with APEX1. Identified in a complex with DEAF1 and XRCC6. Interacts with NR4A3; the DNA-dependent protein kinase complex DNA-PK phosphorylates and activates NR4A3 and prevents NR4A3 ubiquitinylation and degradation. Interacts with RNF138. Interacts with CYREN isoform 1 (CYREN-1) and isoform 4 (CYREN-2). Interacts (via N-terminus) with HSF1 (via N-terminus); this interaction is direct and prevents XRCC5/XRCC6 heterodimeric binding and non-homologous end joining (NHEJ) repair activities induced by ionizing radiation (IR). Interacts with DHX9; this interaction occurs in a RNA-dependent manner. Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA. Interacts with ERCC6.

(Microbial infection) Interacts with human T-cell leukemia virus 1/HTLV-1 protein HBZ.


The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.

Belongs to the ku80 family.

Research Fields

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

Restrictive clause


Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.